{"id":{"repo_id":"brockport","oai_identifier":"oai:soar.suny.edu:20.500.12648/4558"},"canonical_url":"https://search.dev.ndltd.org/etd/brockport/oai:soar.suny.edu:20.500.12648/4558","repository":{"repo_id":"brockport","name":"College at Brockport","base_url":"https://soar.suny.edu/oai/request/"},"display":{"title":"Melanin-Concentrating Hormone Receptor-1 is Enriched in Lipid Rafts and the Effects of Lipid Raft Integrity on Receptor Signaling","abstract":"The melanin-concentrating hormone receptor-1 (MCHR-1) is a member of the G protein-coupled receptor (GPCR) superfamily, and functions in the regulation of food consumption and energy metabolism. MCHR-1 is expressed in neural, pancreatic, and fat tissue. Fat cells begin their journey as pre-adipocytes, culminating in the formation of mature adipocytes as differentiation occurs. Of interest to note, fat cells accumulate caveolae markers -caveolin-1 and cholesterol -during this process. Certain GPCRs and their associated downstream signaling molecules co-localize with caveolae membranes. This thesis seeks to demonstrate that MCHR-1 is enriched in lipid rafts and that a possible consequence of this may be altered signal transduction in obese individuals, such as downregulated ERK 1/2-mediated leptin transcription. Increased amounts of adipose tissue, and thus caveolae, may play a central role in the regulation of MCHR-1 signaling. The first aim of this project addressed the question of MCHR-1 localization to lipid rafts. To test this, caveolae membranes were isolated via sucrose density gradient ultracentrifugation. Subsequent fractionation and western blotting confirmed that MCHR-1 is enriched in lipid raft fractions containing caveolin-1. The second objective assessed ligand dependence on MCHR-1 localization. MCH exposure (1.0-µM) had no obvious effect on receptor localization to lipid raft fractions containing caveolin-1 over an expanded time course. The third aim examined the effect of lipid rafts on MCHR-1 signaling. Pharmacological disruption of caveolae by cholesterol depletion with methyl­ ?-cyclodextrin (M?CD) dampened MCH-mediated ERK 1/2 activation, suggesting that lipid rafts may significantly impact the regulation of MCHR-1 signaling in cells.","abstract_html":"The melanin-concentrating hormone receptor-1 (MCHR-1) is a member of the G protein-coupled receptor (GPCR) superfamily, and functions in the regulation of food consumption and energy metabolism. MCHR-1 is expressed in neural, pancreatic, and fat tissue. Fat cells begin their journey as pre-adipocytes, culminating in the formation of mature adipocytes as differentiation occurs. Of interest to note, fat cells accumulate caveolae markers -caveolin-1 and cholesterol -during this process. Certain GPCRs and their associated downstream signaling molecules co-localize with caveolae membranes. This thesis seeks to demonstrate that MCHR-1 is enriched in lipid rafts and that a possible consequence of this may be altered signal transduction in obese individuals, such as downregulated ERK 1/2-mediated leptin transcription. Increased amounts of adipose tissue, and thus caveolae, may play a central role in the regulation of MCHR-1 signaling. The first aim of this project addressed the question of MCHR-1 localization to lipid rafts. To test this, caveolae membranes were isolated via sucrose density gradient ultracentrifugation. Subsequent fractionation and western blotting confirmed that MCHR-1 is enriched in lipid raft fractions containing caveolin-1. The second objective assessed ligand dependence on MCHR-1 localization. MCH exposure (1.0-µM) had no obvious effect on receptor localization to lipid raft fractions containing caveolin-1 over an expanded time course. The third aim examined the effect of lipid rafts on MCHR-1 signaling. Pharmacological disruption of caveolae by cholesterol depletion with methyl­ ?-cyclodextrin (M?CD) dampened MCH-mediated ERK 1/2 activation, suggesting that lipid rafts may significantly impact the regulation of MCHR-1 signaling in cells.","abstract_has_math":false,"creators":["Delorme-Axford, Elizabeth"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008-07-01","date_published":"2008-07-01","updated_at":"2026-07-27T19:03:56Z","subjects":[],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/20.500.12648/4558","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Delorme-Axford, Elizabeth"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2021-09-07T21:02:54Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2021-09-07T21:02:54Z"]},{"key":"dc:date.issued","label":"Date","values":["2008-07-01"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/20.500.12648/4558"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The melanin-concentrating hormone receptor-1 (MCHR-1) is a member of the G protein-coupled receptor (GPCR) superfamily, and functions in the regulation of food consumption and energy metabolism. MCHR-1 is expressed in neural, pancreatic, and fat tissue. Fat cells begin their journey as pre-adipocytes, culminating in the formation of mature adipocytes as differentiation occurs. Of interest to note, fat cells accumulate caveolae markers -caveolin-1 and cholesterol -during this process. Certain GPCRs and their associated downstream signaling molecules co-localize with caveolae membranes. This thesis seeks to demonstrate that MCHR-1 is enriched in lipid rafts and that a possible consequence of this may be altered signal transduction in obese individuals, such as downregulated ERK 1/2-mediated leptin transcription. Increased amounts of adipose tissue, and thus caveolae, may play a central role in the regulation of MCHR-1 signaling. The first aim of this project addressed the question of MCHR-1 localization to lipid rafts. To test this, caveolae membranes were isolated via sucrose density gradient ultracentrifugation. Subsequent fractionation and western blotting confirmed that MCHR-1 is enriched in lipid raft fractions containing caveolin-1. The second objective assessed ligand dependence on MCHR-1 localization. MCH exposure (1.0-µM) had no obvious effect on receptor localization to lipid raft fractions containing caveolin-1 over an expanded time course. The third aim examined the effect of lipid rafts on MCHR-1 signaling. Pharmacological disruption of caveolae by cholesterol depletion with methyl­ ?-cyclodextrin (M?CD) dampened MCH-mediated ERK 1/2 activation, suggesting that lipid rafts may significantly impact the regulation of MCHR-1 signaling in cells."]},{"key":"dc:title","label":"Title","values":["Melanin-Concentrating Hormone Receptor-1 is Enriched in Lipid Rafts and the Effects of Lipid Raft Integrity on Receptor Signaling"]}]}],"canonical_facts":{"dc:creator":["Delorme-Axford, Elizabeth"],"dc:date.accessioned":["2021-09-07T21:02:54Z"],"dc:date.available":["2021-09-07T21:02:54Z"],"dc:date.issued":["2008-07-01"],"dc:description.abstract":["The melanin-concentrating hormone receptor-1 (MCHR-1) is a member of the G protein-coupled receptor (GPCR) superfamily, and functions in the regulation of food consumption and energy metabolism. MCHR-1 is expressed in neural, pancreatic, and fat tissue. Fat cells begin their journey as pre-adipocytes, culminating in the formation of mature adipocytes as differentiation occurs. Of interest to note, fat cells accumulate caveolae markers -caveolin-1 and cholesterol -during this process. Certain GPCRs and their associated downstream signaling molecules co-localize with caveolae membranes. This thesis seeks to demonstrate that MCHR-1 is enriched in lipid rafts and that a possible consequence of this may be altered signal transduction in obese individuals, such as downregulated ERK 1/2-mediated leptin transcription. Increased amounts of adipose tissue, and thus caveolae, may play a central role in the regulation of MCHR-1 signaling. The first aim of this project addressed the question of MCHR-1 localization to lipid rafts. To test this, caveolae membranes were isolated via sucrose density gradient ultracentrifugation. Subsequent fractionation and western blotting confirmed that MCHR-1 is enriched in lipid raft fractions containing caveolin-1. The second objective assessed ligand dependence on MCHR-1 localization. MCH exposure (1.0-µM) had no obvious effect on receptor localization to lipid raft fractions containing caveolin-1 over an expanded time course. The third aim examined the effect of lipid rafts on MCHR-1 signaling. Pharmacological disruption of caveolae by cholesterol depletion with methyl­ ?-cyclodextrin (M?CD) dampened MCH-mediated ERK 1/2 activation, suggesting that lipid rafts may significantly impact the regulation of MCHR-1 signaling in cells."],"dc:identifier.uri":["http://hdl.handle.net/20.500.12648/4558"],"dc:language.iso":["en_US"],"dc:title":["Melanin-Concentrating Hormone Receptor-1 is Enriched in Lipid Rafts and the Effects of Lipid Raft Integrity on Receptor Signaling"],"dc:type":["thesis"]},"updated_at":"2026-07-27T19:03:56Z"}