{"id":{"repo_id":"brockport","oai_identifier":"oai:soar.suny.edu:20.500.12648/4526"},"canonical_url":"https://search.dev.ndltd.org/etd/brockport/oai:soar.suny.edu:20.500.12648/4526","repository":{"repo_id":"brockport","name":"College at Brockport","base_url":"https://soar.suny.edu/oai/request/"},"display":{"title":"W-reactivation (Inducible \"SOS\" DNA Repair) of double stranded DNA bacteriophage ? and single stranded DNA bacteriophage fd on isogenic rec and uvrB mutants of Escherichia coli K-12","abstract":"The recA mutant has been shown to be completely recombination deficient and highly UV-sensitive. Also, this mutant is remarkably deficient in inducible \"SOS\" DNA repair and, consequently it is not UV-mutable and it cannot perform W-reactivation, an inducible non-excision repair dependent enhancement of phage recovery. The recB-recF- double mutant like the recA mutant, is recombination deficient and UV-sensitive. As observed, each of these mutations appear to block an independent pathway of genetic recombination. We are interested in determining how closely the recB-recF- double mutant resembles the recA mutant. In this perspective we looked at the W-reactivation of double stranded bacteriophage ? and single stranded bacteriophage fd. On examining the W-reactivation for phage ?, it is seen that the recB- and recF- mutants separately lead to a reduction of UV-reactivation efficiency but when spliced, the recB-recF- double mutant further leads to a reduction of W-reactivation even though this is still significant in magnitude when compared to the results obtained for the recA mutant. In the recA mutant W-reactivation capability is totally absent. Contrary to our results with bacteriophage ? , the recB- and recF mutants individually show enhanced W-reactivation of fd phage. The double mutant recB-recF- however, shows virtually no UV-reactivation potential. This is the same case for recA- mutant. Host-cell reactivation - an excision repair dependent potential was examined in the fd phage, ? vir and Pl vira. Our results demonstrate that host-cell recovery is non-existent in fd phage. On the other hand, we noticed high levels of phage recovery in ? vir and Pl vira. We have demonstrated that although recB-recF- double mutant closely resembles the recA single mutant in their UV-sensitivity and recombination profile, this resemblance is seen to be parallel when their UV-inducible capability is examined in double stranded bacteriophages. We therefore conclude that there are significant levels of inducible \"SOS\" DNA repair occurring in the recB- and recF- mutants and not in the double mutant, recB-recF-. This is due to the fact that there are genetic differences in the inducible DNA repair capability of single stranded and double stranded bacteriophages.","abstract_html":"The recA mutant has been shown to be completely recombination deficient and highly UV-sensitive. Also, this mutant is remarkably deficient in inducible "SOS" DNA repair and, consequently it is not UV-mutable and it cannot perform W-reactivation, an inducible non-excision repair dependent enhancement of phage recovery. The recB-recF- double mutant like the recA mutant, is recombination deficient and UV-sensitive. As observed, each of these mutations appear to block an independent pathway of genetic recombination. We are interested in determining how closely the recB-recF- double mutant resembles the recA mutant. In this perspective we looked at the W-reactivation of double stranded bacteriophage ? and single stranded bacteriophage fd. On examining the W-reactivation for phage ?, it is seen that the recB- and recF- mutants separately lead to a reduction of UV-reactivation efficiency but when spliced, the recB-recF- double mutant further leads to a reduction of W-reactivation even though this is still significant in magnitude when compared to the results obtained for the recA mutant. In the recA mutant W-reactivation capability is totally absent. Contrary to our results with bacteriophage ? , the recB- and recF mutants individually show enhanced W-reactivation of fd phage. The double mutant recB-recF- however, shows virtually no UV-reactivation potential. This is the same case for recA- mutant. Host-cell reactivation - an excision repair dependent potential was examined in the fd phage, ? vir and Pl vira. Our results demonstrate that host-cell recovery is non-existent in fd phage. On the other hand, we noticed high levels of phage recovery in ? vir and Pl vira. We have demonstrated that although recB-recF- double mutant closely resembles the recA single mutant in their UV-sensitivity and recombination profile, this resemblance is seen to be parallel when their UV-inducible capability is examined in double stranded bacteriophages. We therefore conclude that there are significant levels of inducible "SOS" DNA repair occurring in the recB- and recF- mutants and not in the double mutant, recB-recF-. This is due to the fact that there are genetic differences in the inducible DNA repair capability of single stranded and double stranded bacteriophages.","abstract_has_math":false,"creators":["Ndive, Sammy F.W."],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Rothman, Robert H."],"committee_chairs":[],"committee_members":[],"year":1983,"date_issued":"1983-05-01","date_published":"1983-05-01","updated_at":"2026-07-27T19:02:26Z","subjects":[],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/20.500.12648/4526","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Rothman, Robert H."]},{"key":"dc:creator","label":"Author","values":["Ndive, Sammy F.W."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2021-09-07T21:02:48Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2021-09-07T21:02:48Z"]},{"key":"dc:date.issued","label":"Date","values":["1983-05-01"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/20.500.12648/4526"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The recA mutant has been shown to be completely recombination deficient and highly UV-sensitive. Also, this mutant is remarkably deficient in inducible \"SOS\" DNA repair and, consequently it is not UV-mutable and it cannot perform W-reactivation, an inducible non-excision repair dependent enhancement of phage recovery. The recB-recF- double mutant like the recA mutant, is recombination deficient and UV-sensitive. As observed, each of these mutations appear to block an independent pathway of genetic recombination. We are interested in determining how closely the recB-recF- double mutant resembles the recA mutant. In this perspective we looked at the W-reactivation of double stranded bacteriophage ? and single stranded bacteriophage fd. On examining the W-reactivation for phage ?, it is seen that the recB- and recF- mutants separately lead to a reduction of UV-reactivation efficiency but when spliced, the recB-recF- double mutant further leads to a reduction of W-reactivation even though this is still significant in magnitude when compared to the results obtained for the recA mutant. In the recA mutant W-reactivation capability is totally absent. Contrary to our results with bacteriophage ? , the recB- and recF mutants individually show enhanced W-reactivation of fd phage. The double mutant recB-recF- however, shows virtually no UV-reactivation potential. This is the same case for recA- mutant. Host-cell reactivation - an excision repair dependent potential was examined in the fd phage, ? vir and Pl vira. Our results demonstrate that host-cell recovery is non-existent in fd phage. On the other hand, we noticed high levels of phage recovery in ? vir and Pl vira. We have demonstrated that although recB-recF- double mutant closely resembles the recA single mutant in their UV-sensitivity and recombination profile, this resemblance is seen to be parallel when their UV-inducible capability is examined in double stranded bacteriophages. We therefore conclude that there are significant levels of inducible \"SOS\" DNA repair occurring in the recB- and recF- mutants and not in the double mutant, recB-recF-. This is due to the fact that there are genetic differences in the inducible DNA repair capability of single stranded and double stranded bacteriophages."]},{"key":"dc:title","label":"Title","values":["W-reactivation (Inducible \"SOS\" DNA Repair) of double stranded DNA bacteriophage ? and single stranded DNA bacteriophage fd on isogenic rec and uvrB mutants of Escherichia coli K-12"]}]}],"canonical_facts":{"dc:contributor.advisor":["Rothman, Robert H."],"dc:creator":["Ndive, Sammy F.W."],"dc:date.accessioned":["2021-09-07T21:02:48Z"],"dc:date.available":["2021-09-07T21:02:48Z"],"dc:date.issued":["1983-05-01"],"dc:description.abstract":["The recA mutant has been shown to be completely recombination deficient and highly UV-sensitive. Also, this mutant is remarkably deficient in inducible \"SOS\" DNA repair and, consequently it is not UV-mutable and it cannot perform W-reactivation, an inducible non-excision repair dependent enhancement of phage recovery. The recB-recF- double mutant like the recA mutant, is recombination deficient and UV-sensitive. As observed, each of these mutations appear to block an independent pathway of genetic recombination. We are interested in determining how closely the recB-recF- double mutant resembles the recA mutant. In this perspective we looked at the W-reactivation of double stranded bacteriophage ? and single stranded bacteriophage fd. On examining the W-reactivation for phage ?, it is seen that the recB- and recF- mutants separately lead to a reduction of UV-reactivation efficiency but when spliced, the recB-recF- double mutant further leads to a reduction of W-reactivation even though this is still significant in magnitude when compared to the results obtained for the recA mutant. In the recA mutant W-reactivation capability is totally absent. Contrary to our results with bacteriophage ? , the recB- and recF mutants individually show enhanced W-reactivation of fd phage. The double mutant recB-recF- however, shows virtually no UV-reactivation potential. This is the same case for recA- mutant. Host-cell reactivation - an excision repair dependent potential was examined in the fd phage, ? vir and Pl vira. Our results demonstrate that host-cell recovery is non-existent in fd phage. On the other hand, we noticed high levels of phage recovery in ? vir and Pl vira. We have demonstrated that although recB-recF- double mutant closely resembles the recA single mutant in their UV-sensitivity and recombination profile, this resemblance is seen to be parallel when their UV-inducible capability is examined in double stranded bacteriophages. We therefore conclude that there are significant levels of inducible \"SOS\" DNA repair occurring in the recB- and recF- mutants and not in the double mutant, recB-recF-. This is due to the fact that there are genetic differences in the inducible DNA repair capability of single stranded and double stranded bacteriophages."],"dc:identifier.uri":["http://hdl.handle.net/20.500.12648/4526"],"dc:language.iso":["en_US"],"dc:title":["W-reactivation (Inducible \"SOS\" DNA Repair) of double stranded DNA bacteriophage ? and single stranded DNA bacteriophage fd on isogenic rec and uvrB mutants of Escherichia coli K-12"],"dc:type":["thesis"]},"updated_at":"2026-07-27T19:02:26Z"}