Characterization of Transfer RNA Associated with Plasma Membranes
RNA extracted from purified rat liver plasma membranes was found to contain transfer RNA. Amino acid acceptor activity was detected for arginine and lysine, a confirmation of previous reports. In addition, acceptor activity was also detected for tryptophan and proline. Isoaccepting tRNA species for lysine and arginine were chromatographed using Benzoylated DEAE-Cellulose column chromatography. No major difference was found between cytoplasmic and membrane isoaccepting species for lysine and arginine transfer RNAs.
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