1,25- dihydroxycholecalciferol-glycoside supplementation for LPS - challenged broiler chickens

This study evaluates the effects of dietary supplementation of 1,25-dihydroxycholecalciferol (1,25(OH) 2 D 3 ) glycoside on performance, tibia mineral concentration, and inflammatory responses of broilers challenged with lipopolysaccharides (LPS). At 20 days old, 144 male broilers were weighed and distributed in a completely randomized design with three treatments, eight replicates, and six birds per experimental unit. The treatments consisted in a basal diet without LPS administration (CON), a basal diet with LPS administration (CON+LPS), and a basal diet supplemented with 5 μg of 1,25(OH) 2 D 3 -glycoside/kg of diet with LPS administration (SUP+LPS). The LPS challenge was administered as intraperitoneal injections at 21 and 23 days of age (1 and 1.12 mg/kg of body weight, respectively). Four hours after the first LPS injection, one bird per experimental unit was euthanized to collect blood, spleen, duodenum, and jejunum samples. Broilers performance was evaluated at 25 days of age, when all birds were slaughtered to analyze calcium and phosphorus cocentrations in the tibia. Data were analyzed using a one-way ANOVA and adjusted means were compared using the Tukey test at 0.05 significance. The CON+LPS birds had less weight gain (WG) than CON and SUP+LPS groups (P = 0.019). Treatments had no effect on feed intake, feed conversion, serum levels of bone alkaline phosphatase, calcium, and phosphorus, and calcium and phosphorus concentration in the tibia (P > 0.05). The IL-10 level in the jejunum was higher (P = 0.011) in SUP+LPS birds than in the CON and CON+LPS groups. Among birds challenged with LPS, those supplemented with 1,25(OH) 2 D 3 -glycoside showed greater mRNA expression of IL-10 (P = 0.014) and IL-1β (P = 0.045) in the spleen; however, no effect was observed on the expression of these cytokines in the duodenum and jejunum of the birds (P > 0.05). In conclusion, dietary supplementation with 1,25(OH) 2 D 3 -glycoside preserves WG and modulates the inflammatory response by increasing the production of IL-10 in the jejunum and the mRNA expression of IL-10 and IL-1β in the spleens of broilers challenged with LPS, although bone mineralization is unaffected. Keywords: Inflammation. Performance. Poultry. Vitamin D.

Grantor dc:publisher

Universidade Federal de Viçosa

Year dc:date.issued

2020

Author dc:creator

• Nunes, Rayanne Andrade

Advisor dc:contributor.advisor

• Calderano, Arele Arlindo

Contributors dc:contributor

• Duarte, Marcio de Souza
• Campos, Paulo Henrique Reis Furtado

• Frango de corte - Alimentação e rações
• Desempenho - Avaliação
• Inflamação
• Vitamina D

Statement dc:rights

• Acesso Aberto

Language dc:language.iso

eng

Repository record dc:identifier.uri

https://locus.ufv.br//handle/123456789/29408

OAI identifier oai:identifier

oai:locus.ufv.br:123456789/29408