{"id":{"repo_id":"brazil-ufba","oai_identifier":"oai:repositorio.ufba.br:ri/39954"},"canonical_url":"https://search.dev.ndltd.org/etd/brazil-ufba/oai:repositorio.ufba.br:ri/39954","repository":{"repo_id":"brazil-ufba","name":"Brazil UFBA","base_url":"https://repositorio.ufba.br/oai/request"},"display":{"title":"CONCENTRAÇÕES ELEVADAS DAS CITOCINAS ASSOCIADAS À RESPOSTA Th17 E DESFECHOS DESFAVORÁVEIS EM PACIENTES COM COVID-19 ADMITIDOS À UNIDADE DE TERAPIA INTENSIVA","abstract":"COVID-19 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is now understood that the immune response plays a crucial role in modulating the severity and outcome of this disease. In this context, the Th17 response has been receiving increasing attention due to its ability to modulate inflammation. A prospective inclusion of 56 COVID-19 patients admitted to the Intensive Care Unit (ICU) of the General Hospital of Vitória da Conquista (HGVC) in Bahia, Brazil, was conducted. All included patients had SARS-CoV-2 infection confirmed through real-time polymerase chain reaction (RT-PCR) of nasopharyngeal swab samples. Serum samples were collected from all participants to determine the concentration of Th17-associated cytokines (IL-1β, TGF-β, IL-6, IL-17, and IL-23) using enzyme-linked immunosorbent assay (ELISA). The Shapiro-Wilk test assessed the normality of data distribution, and Levene's test verified variance homogeneity. Student's t-test was employed for mean comparisons, and chi-square independence tests (2x2) examined associations between categorical variables. Lastly, Spearman correlation evaluated associations between cytokines and the mortality outcome variable. It was initially demonstrated that male patients had a higher likelihood of mortality compared to female patients. Deceased patients exhibited higher levels of IL-1β and IL-6 compared to those discharged, while TGF-β levels in deceased patients were substantially elevated. Although statistically significant differences were not observed, there was a trend of higher levels of IL-17 and IL-23 in patients who succumbed to mortality. IL-1β and TGF-β showed a significant strong correlation with the mortality outcome, while IL-6 presented a moderate correlation. Strong significant correlations were found between IL-1β and TGF-β, as well as between IL-6 and IL-17, and IL-6 and IL-23, with a moderate correlation observed between IL-6 and TGF-β. Finally, IL-17 and IL-23 exhibited a significant correlation with each other. Based on our analysis, we conclude that elevated levels of cytokines IL-1β, TGF-β, and IL-6 are significantly associated with an unfavorable outcome in SARS-CoV-2 patients, irrespective of comorbidities. These cytokines may play a crucial role in the pathogenesis of the disease and serve as useful indicators for assessing the severity and prognosis of SARS-CoV-2-infected patients.","abstract_html":"COVID-19 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is now understood that the immune response plays a crucial role in modulating the severity and outcome of this disease. In this context, the Th17 response has been receiving increasing attention due to its ability to modulate inflammation. A prospective inclusion of 56 COVID-19 patients admitted to the Intensive Care Unit (ICU) of the General Hospital of Vitória da Conquista (HGVC) in Bahia, Brazil, was conducted. All included patients had SARS-CoV-2 infection confirmed through real-time polymerase chain reaction (RT-PCR) of nasopharyngeal swab samples. Serum samples were collected from all participants to determine the concentration of Th17-associated cytokines (IL-1β, TGF-β, IL-6, IL-17, and IL-23) using enzyme-linked immunosorbent assay (ELISA). The Shapiro-Wilk test assessed the normality of data distribution, and Levene's test verified variance homogeneity. Student's t-test was employed for mean comparisons, and chi-square independence tests (2x2) examined associations between categorical variables. Lastly, Spearman correlation evaluated associations between cytokines and the mortality outcome variable. It was initially demonstrated that male patients had a higher likelihood of mortality compared to female patients. Deceased patients exhibited higher levels of IL-1β and IL-6 compared to those discharged, while TGF-β levels in deceased patients were substantially elevated. Although statistically significant differences were not observed, there was a trend of higher levels of IL-17 and IL-23 in patients who succumbed to mortality. IL-1β and TGF-β showed a significant strong correlation with the mortality outcome, while IL-6 presented a moderate correlation. Strong significant correlations were found between IL-1β and TGF-β, as well as between IL-6 and IL-17, and IL-6 and IL-23, with a moderate correlation observed between IL-6 and TGF-β. Finally, IL-17 and IL-23 exhibited a significant correlation with each other. Based on our analysis, we conclude that elevated levels of cytokines IL-1β, TGF-β, and IL-6 are significantly associated with an unfavorable outcome in SARS-CoV-2 patients, irrespective of comorbidities. These cytokines may play a crucial role in the pathogenesis of the disease and serve as useful indicators for assessing the severity and prognosis of SARS-CoV-2-infected patients.","abstract_has_math":false,"creators":["Lopes, Luana Weber"],"institution":"Universidade Federal da Bahia","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-03-15","date_published":"2024-03-15","updated_at":"2026-07-27T22:07:41Z","subjects":["SARS-CoV-2","Resposta Th 17","Citocinas"],"languages":["por"],"rights":["Acesso Aberto"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://repositorio.ufba.br/handle/ri/39954","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Lopes, Luana Weber"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2024-08-22T13:34:50Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2024-08-22T13:34:50Z"]},{"key":"dc:date.issued","label":"Date","values":["2024-03-15"]},{"key":"dc:publisher","label":"Institution","values":["Universidade Federal da Bahia"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Instituto Multidisciplinar em Saúde (IMS)"]},{"key":"dc:type","label":"Dc Type","values":["Dissertação"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["SARS-CoV-2","Resposta Th 17","Citocinas"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["por"]},{"key":"dc:rights","label":"Dc Rights","values":["Acesso Aberto"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://repositorio.ufba.br/handle/ri/39954"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["COVID-19 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is now understood that the immune response plays a crucial role in modulating the severity and outcome of this disease. In this context, the Th17 response has been receiving increasing attention due to its ability to modulate inflammation. A prospective inclusion of 56 COVID-19 patients admitted to the Intensive Care Unit (ICU) of the General Hospital of Vitória da Conquista (HGVC) in Bahia, Brazil, was conducted. All included patients had SARS-CoV-2 infection confirmed through real-time polymerase chain reaction (RT-PCR) of nasopharyngeal swab samples. Serum samples were collected from all participants to determine the concentration of Th17-associated cytokines (IL-1β, TGF-β, IL-6, IL-17, and IL-23) using enzyme-linked immunosorbent assay (ELISA). The Shapiro-Wilk test assessed the normality of data distribution, and Levene's test verified variance homogeneity. Student's t-test was employed for mean comparisons, and chi-square independence tests (2x2) examined associations between categorical variables. Lastly, Spearman correlation evaluated associations between cytokines and the mortality outcome variable. It was initially demonstrated that male patients had a higher likelihood of mortality compared to female patients. Deceased patients exhibited higher levels of IL-1β and IL-6 compared to those discharged, while TGF-β levels in deceased patients were substantially elevated. Although statistically significant differences were not observed, there was a trend of higher levels of IL-17 and IL-23 in patients who succumbed to mortality. IL-1β and TGF-β showed a significant strong correlation with the mortality outcome, while IL-6 presented a moderate correlation. Strong significant correlations were found between IL-1β and TGF-β, as well as between IL-6 and IL-17, and IL-6 and IL-23, with a moderate correlation observed between IL-6 and TGF-β. Finally, IL-17 and IL-23 exhibited a significant correlation with each other. Based on our analysis, we conclude that elevated levels of cytokines IL-1β, TGF-β, and IL-6 are significantly associated with an unfavorable outcome in SARS-CoV-2 patients, irrespective of comorbidities. These cytokines may play a crucial role in the pathogenesis of the disease and serve as useful indicators for assessing the severity and prognosis of SARS-CoV-2-infected patients."]},{"key":"dc:title","label":"Title","values":["CONCENTRAÇÕES ELEVADAS DAS CITOCINAS ASSOCIADAS À RESPOSTA Th17 E DESFECHOS DESFAVORÁVEIS EM PACIENTES COM COVID-19 ADMITIDOS À UNIDADE DE TERAPIA INTENSIVA"]}]}],"canonical_facts":{"dc:creator":["Lopes, Luana Weber"],"dc:date.accessioned":["2024-08-22T13:34:50Z"],"dc:date.available":["2024-08-22T13:34:50Z"],"dc:date.issued":["2024-03-15"],"dc:description.abstract":["COVID-19 is an infectious disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It is now understood that the immune response plays a crucial role in modulating the severity and outcome of this disease. In this context, the Th17 response has been receiving increasing attention due to its ability to modulate inflammation. A prospective inclusion of 56 COVID-19 patients admitted to the Intensive Care Unit (ICU) of the General Hospital of Vitória da Conquista (HGVC) in Bahia, Brazil, was conducted. All included patients had SARS-CoV-2 infection confirmed through real-time polymerase chain reaction (RT-PCR) of nasopharyngeal swab samples. Serum samples were collected from all participants to determine the concentration of Th17-associated cytokines (IL-1β, TGF-β, IL-6, IL-17, and IL-23) using enzyme-linked immunosorbent assay (ELISA). The Shapiro-Wilk test assessed the normality of data distribution, and Levene's test verified variance homogeneity. Student's t-test was employed for mean comparisons, and chi-square independence tests (2x2) examined associations between categorical variables. Lastly, Spearman correlation evaluated associations between cytokines and the mortality outcome variable. It was initially demonstrated that male patients had a higher likelihood of mortality compared to female patients. Deceased patients exhibited higher levels of IL-1β and IL-6 compared to those discharged, while TGF-β levels in deceased patients were substantially elevated. Although statistically significant differences were not observed, there was a trend of higher levels of IL-17 and IL-23 in patients who succumbed to mortality. IL-1β and TGF-β showed a significant strong correlation with the mortality outcome, while IL-6 presented a moderate correlation. Strong significant correlations were found between IL-1β and TGF-β, as well as between IL-6 and IL-17, and IL-6 and IL-23, with a moderate correlation observed between IL-6 and TGF-β. Finally, IL-17 and IL-23 exhibited a significant correlation with each other. Based on our analysis, we conclude that elevated levels of cytokines IL-1β, TGF-β, and IL-6 are significantly associated with an unfavorable outcome in SARS-CoV-2 patients, irrespective of comorbidities. These cytokines may play a crucial role in the pathogenesis of the disease and serve as useful indicators for assessing the severity and prognosis of SARS-CoV-2-infected patients."],"dc:identifier.uri":["https://repositorio.ufba.br/handle/ri/39954"],"dc:language":["por"],"dc:publisher":["Universidade Federal da Bahia"],"dc:publisher.department":["Instituto Multidisciplinar em Saúde (IMS)"],"dc:rights":["Acesso Aberto"],"dc:subject":["SARS-CoV-2","Resposta Th 17","Citocinas"],"dc:title":["CONCENTRAÇÕES ELEVADAS DAS CITOCINAS ASSOCIADAS À RESPOSTA Th17 E DESFECHOS DESFAVORÁVEIS EM PACIENTES COM COVID-19 ADMITIDOS À UNIDADE DE TERAPIA INTENSIVA"],"dc:type":["Dissertação"]},"updated_at":"2026-07-27T22:07:41Z"}