{"id":{"repo_id":"bradford","oai_identifier":"oai:bradscholars.brad.ac.uk:10454/20543"},"canonical_url":"https://search.dev.ndltd.org/etd/bradford/oai:bradscholars.brad.ac.uk:10454/20543","repository":{"repo_id":"bradford","name":"University of Bradford","base_url":"https://bradscholars.brad.ac.uk/oai/request"},"display":{"title":"Modulation of human adipose-derived stem cells and dermal fibroblasts in co-cultures by activation of the IL-6 trans-signalling pathway via the soluble IL-6Rα receptor","abstract":"Interleukin-6 (IL-6), a major wound healing cytokine, has rare, versatile agonistic effects, due to dual pro and anti-inflammatory roles depending on the intracellular signalling pathway. The aim was to evaluate the relationship between human dermal fibroblasts (DF), human adipose-derived stem cells (ADSC), IL-6 and activation of the IL-6 trans-signalling pathway under four different cell culture conditions. The two cell types were co-cultured to assess cell migration when pre-treated with the soluble human IL-6Rα receptor (rhIL-6Rα). In addition, secreted cytokine levels (TGFβ1, TGFβ3 and VEGF) and biomarker expression (TGF β1RII, SMAD2/3, SMAD 1/5/9 and SMA) were quantitated. Although both cell types secreted IL-6, ADSC basal levels were 100-fold that of DF. Both cell types expressed CD130 (gp130) signalling transductor but were negative for the membrane-bound CD126 receptor required for the classical IL-6 signalling pathway. Following IL-6 trans-signalling pathway activation with rhIL-6Rα, ADSC migrated predominantly, while DF acted more as attractants. Moreover, the overall secretion of TGFβ3 and VEGF, and the ratio of TGFβ3/TGFβ1 showed similar variations which suggested they increased, although not statistically significant and were maintained at 72h, but only in co-cultures where the ADSC were pre-treated. When cells were pre-treated with rhIL-6Rα, the overall expression of TGFβRII and SMAD2/3 increased suggesting crosstalk between TGFβ1 and IL-6. In summary, these results suggest that activation of the IL-6 trans-signalling pathway modulates ADSC plasticity impacting on migration. Thus, further research is required to assess the use of ADSC in concurrence with manipulation of the IL-6 signalling pathway in wound management.","abstract_html":"Interleukin-6 (IL-6), a major wound healing cytokine, has rare, versatile agonistic effects, due to dual pro and anti-inflammatory roles depending on the intracellular signalling pathway. The aim was to evaluate the relationship between human dermal fibroblasts (DF), human adipose-derived stem cells (ADSC), IL-6 and activation of the IL-6 trans-signalling pathway under four different cell culture conditions. The two cell types were co-cultured to assess cell migration when pre-treated with the soluble human IL-6Rα receptor (rhIL-6Rα). In addition, secreted cytokine levels (TGFβ1, TGFβ3 and VEGF) and biomarker expression (TGF β1RII, SMAD2/3, SMAD 1/5/9 and SMA) were quantitated. Although both cell types secreted IL-6, ADSC basal levels were 100-fold that of DF. Both cell types expressed CD130 (gp130) signalling transductor but were negative for the membrane-bound CD126 receptor required for the classical IL-6 signalling pathway. Following IL-6 trans-signalling pathway activation with rhIL-6Rα, ADSC migrated predominantly, while DF acted more as attractants. Moreover, the overall secretion of TGFβ3 and VEGF, and the ratio of TGFβ3/TGFβ1 showed similar variations which suggested they increased, although not statistically significant and were maintained at 72h, but only in co-cultures where the ADSC were pre-treated. When cells were pre-treated with rhIL-6Rα, the overall expression of TGFβRII and SMAD2/3 increased suggesting crosstalk between TGFβ1 and IL-6. In summary, these results suggest that activation of the IL-6 trans-signalling pathway modulates ADSC plasticity impacting on migration. Thus, further research is required to assess the use of ADSC in concurrence with manipulation of the IL-6 signalling pathway in wound management.","abstract_has_math":false,"creators":["Chelmuș, Alina"],"institution":"University of Bradford","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Thornton, M. Julie","Riches-Suman, Kirsten"],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T01:14:55Z","subjects":["Wound healing","Soluble IL-6Rα receptor","Adipose-derived stem cells","Dermal fibroblasts","Migration","IL-6 trans-signalling pathway","Interleukin-6 (IL-6)"],"languages":["en"],"rights":["\"Creative
The University of Bradford theses are licenced under a Creative Commons Licence."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bradscholars.brad.ac.uk/handle/10454/20543","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Thornton, M. Julie","Riches-Suman, Kirsten"]},{"key":"dc:creator","label":"Author","values":["Chelmuș, Alina"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-30T15:46:49Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-30T15:46:49Z"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Chemistry and Biosciences. Faculty of Life Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Bradford"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["research masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MPhil"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Wound healing","Soluble IL-6Rα receptor","Adipose-derived stem cells","Dermal fibroblasts","Migration","IL-6 trans-signalling pathway","Interleukin-6 (IL-6)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["\"Creative
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The University of Bradford theses are licenced under a Creative Commons Licence."],"dc:subject":["Wound healing","Soluble IL-6Rα receptor","Adipose-derived stem cells","Dermal fibroblasts","Migration","IL-6 trans-signalling pathway","Interleukin-6 (IL-6)"],"dc:title":["Modulation of human adipose-derived stem cells and dermal fibroblasts in co-cultures by activation of the IL-6 trans-signalling pathway via the soluble IL-6Rα receptor"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["research masters"],"dc:type.qualificationname":["MPhil"]},"updated_at":"2026-07-24T01:14:55Z"}