{"id":{"repo_id":"birmingham","oai_identifier":"oai:etheses.bham.ac.uk:594"},"canonical_url":"https://search.dev.ndltd.org/etd/birmingham/oai:etheses.bham.ac.uk:594","repository":{"repo_id":"birmingham","name":"University of Birmingham","base_url":"https://etheses.bham.ac.uk/cgi/oai2"},"display":{"title":"Multifunctional chromatography supports","abstract":"The aim of this study was to create a bi-layered packed bed chromatography support for the purification of nano-sized bioproducts. The effect of three different chemistry approaches, different solvent conditions, and microwave heating were investigated on construction of a bi-layered support. Sepharose CL-6B was activated with allyl glycidyl ether (routes 1&2). The inert outer layer was created in route 1 by reacting bromine with the allyl groups at the surface, followed by the addition of sodium hydroxide. Creation of the outer layer in route 2 was achieved by oxidation of surface groups with potassium permanganate. In both synthesises the allyl groups remaining were reacted with bromine and a charged amine ligand was coupled to the inner core. The activation step of route 3 resulted in the introduction of three membered epoxide groups throughout the support. Surface groups were reacted with sodium hydroxide or hydrochloric acid. Finally a charged amine ligand was coupled to the support by reacting trimethylamine hydrochloride with the remaining epoxide groups. Supports created by route 1 eliminated 91% of plasmid DNA binding whilst maintaining a high protein binding capacity. This was achieved by using DMS0 as the solvent in the bromination step and employing microwave heating. Route 2 proved to be the least successful in creating a bi-layered support. The beads created under hydrochloric acid-methanol conditions (route 3) reduced 91% of the plasmid DNA binding whilst maintaining a high protein binding capacity. This study revealed that microwave heating was a useful tool in the synthesis of chromatography supports. Subsequently, a comprehensive study was untaken investigating the effects of microwaves on numerous chromatography matrices.","abstract_html":"The aim of this study was to create a bi-layered packed bed chromatography support for the purification of nano-sized bioproducts. The effect of three different chemistry approaches, different solvent conditions, and microwave heating were investigated on construction of a bi-layered support. Sepharose CL-6B was activated with allyl glycidyl ether (routes 1&2). The inert outer layer was created in route 1 by reacting bromine with the allyl groups at the surface, followed by the addition of sodium hydroxide. Creation of the outer layer in route 2 was achieved by oxidation of surface groups with potassium permanganate. In both synthesises the allyl groups remaining were reacted with bromine and a charged amine ligand was coupled to the inner core. The activation step of route 3 resulted in the introduction of three membered epoxide groups throughout the support. Surface groups were reacted with sodium hydroxide or hydrochloric acid. Finally a charged amine ligand was coupled to the support by reacting trimethylamine hydrochloride with the remaining epoxide groups. Supports created by route 1 eliminated 91% of plasmid DNA binding whilst maintaining a high protein binding capacity. This was achieved by using DMS0 as the solvent in the bromination step and employing microwave heating. Route 2 proved to be the least successful in creating a bi-layered support. The beads created under hydrochloric acid-methanol conditions (route 3) reduced 91% of the plasmid DNA binding whilst maintaining a high protein binding capacity. This study revealed that microwave heating was a useful tool in the synthesis of chromatography supports. Subsequently, a comprehensive study was untaken investigating the effects of microwaves on numerous chromatography matrices.","abstract_has_math":false,"creators":["Liddy, Alison Mary"],"institution":"University of Birmingham","degree_name":"d_ph","degree_level":"d_ph","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-07","date_published":"2010-07","updated_at":"2026-07-24T01:11:02Z","subjects":["TP Chemical technology","QD Chemistry"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.sponsor","label":"Sponsor","values":["na"]},{"key":"dc:creator","label":"Author","values":["Liddy, Alison Mary"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-07"]},{"key":"dc:date.issued","label":"Date","values":["2010-07"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["College of Engineering & Physical Sciences","School of Chemical Engineering"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Birmingham"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["http://etheses.bham.ac.uk//id/eprint/594/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["d_ph"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["d_ph"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["TP Chemical technology","QD Chemistry"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://etheses.bham.ac.uk//id/eprint/594/1/Liddy10PhD.pdf","http://etheses.bham.ac.uk//id/eprint/594/2/Decl_IS_Liddy10PhD.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The aim of this study was to create a bi-layered packed bed chromatography support for the purification of nano-sized bioproducts. The effect of three different chemistry approaches, different solvent conditions, and microwave heating were investigated on construction of a bi-layered support. Sepharose CL-6B was activated with allyl glycidyl ether (routes 1&2). The inert outer layer was created in route 1 by reacting bromine with the allyl groups at the surface, followed by the addition of sodium hydroxide. Creation of the outer layer in route 2 was achieved by oxidation of surface groups with potassium permanganate. In both synthesises the allyl groups remaining were reacted with bromine and a charged amine ligand was coupled to the inner core. The activation step of route 3 resulted in the introduction of three membered epoxide groups throughout the support. Surface groups were reacted with sodium hydroxide or hydrochloric acid. Finally a charged amine ligand was coupled to the support by reacting trimethylamine hydrochloride with the remaining epoxide groups. Supports created by route 1 eliminated 91% of plasmid DNA binding whilst maintaining a high protein binding capacity. This was achieved by using DMS0 as the solvent in the bromination step and employing microwave heating. Route 2 proved to be the least successful in creating a bi-layered support. The beads created under hydrochloric acid-methanol conditions (route 3) reduced 91% of the plasmid DNA binding whilst maintaining a high protein binding capacity. This study revealed that microwave heating was a useful tool in the synthesis of chromatography supports. Subsequently, a comprehensive study was untaken investigating the effects of microwaves on numerous chromatography matrices."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Multifunctional chromatography supports"]}]}],"canonical_facts":{"dc:contributor.sponsor":["na"],"dc:creator":["Liddy, Alison Mary"],"dc:date":["2010-07"],"dc:date.issued":["2010-07"],"dc:description.abstract":["The aim of this study was to create a bi-layered packed bed chromatography support for the purification of nano-sized bioproducts. The effect of three different chemistry approaches, different solvent conditions, and microwave heating were investigated on construction of a bi-layered support. Sepharose CL-6B was activated with allyl glycidyl ether (routes 1&2). The inert outer layer was created in route 1 by reacting bromine with the allyl groups at the surface, followed by the addition of sodium hydroxide. Creation of the outer layer in route 2 was achieved by oxidation of surface groups with potassium permanganate. In both synthesises the allyl groups remaining were reacted with bromine and a charged amine ligand was coupled to the inner core. The activation step of route 3 resulted in the introduction of three membered epoxide groups throughout the support. Surface groups were reacted with sodium hydroxide or hydrochloric acid. Finally a charged amine ligand was coupled to the support by reacting trimethylamine hydrochloride with the remaining epoxide groups. Supports created by route 1 eliminated 91% of plasmid DNA binding whilst maintaining a high protein binding capacity. This was achieved by using DMS0 as the solvent in the bromination step and employing microwave heating. Route 2 proved to be the least successful in creating a bi-layered support. The beads created under hydrochloric acid-methanol conditions (route 3) reduced 91% of the plasmid DNA binding whilst maintaining a high protein binding capacity. This study revealed that microwave heating was a useful tool in the synthesis of chromatography supports. Subsequently, a comprehensive study was untaken investigating the effects of microwaves on numerous chromatography matrices."],"dc:format":["application/pdf"],"dc:identifier.uri":["http://etheses.bham.ac.uk//id/eprint/594/1/Liddy10PhD.pdf","http://etheses.bham.ac.uk//id/eprint/594/2/Decl_IS_Liddy10PhD.pdf"],"dc:publisher.department":["College of Engineering & Physical Sciences","School of Chemical Engineering"],"dc:publisher.institution":["University of Birmingham"],"dc:relation.isreferencedby":["http://etheses.bham.ac.uk//id/eprint/594/"],"dc:subject":["TP Chemical technology","QD Chemistry"],"dc:title":["Multifunctional chromatography supports"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["d_ph"],"dc:type.qualificationname":["d_ph"]},"updated_at":"2026-07-24T01:11:02Z"}