{"id":{"repo_id":"birmingham","oai_identifier":"oai:etheses.bham.ac.uk:194"},"canonical_url":"https://search.dev.ndltd.org/etd/birmingham/oai:etheses.bham.ac.uk:194","repository":{"repo_id":"birmingham","name":"University of Birmingham","base_url":"https://etheses.bham.ac.uk/cgi/oai2"},"display":{"title":"Cell biology of settlement and adhesion processes of biofouling algae","abstract":"The aim of the research presented in this thesis was to investigate the cell biology behind the settlement and adhesion processes of biofouling algae. Using the fluorescent dye FM 1-43 in Ulva zoospores to follow membrane recycling, rapid mass membrane retrieval of FM 1-43-labelled plasma membrane was found to occur to an endosomal compartment during settlement. Biolistic delivery of dextran Oregon Green BAPTA-1 and Texas Red enabled ratiometric imaging with a 5-fold greater response to Ca\\(^{2+}\\)-ionophores than AM-ester Ca\\(^{2+}\\) indicators. During settlement, zoospores exhibited both localised and diffuse increases in cytosolic calcium implying a role in secretion of the adhesive. Secretion of redox-active substrates was detected using amperometry when settled spores were mechano-stimulated. Secretory events were similar to those seen in bovine chromaffin cells with the presence of foot signals in the recordings implying a role for a ‘fusion pore’ in exocytosis. Using DAF-FM DA nitric oxide (NO) production in Seminavis robusta was found to be 4-fold greater on a surface to which the cells adhered weakly than on a surface to which they attached more strongly. Increased NO reduced attachment strength and it is thought that NO may play a signalling and/or regulatory role in diatom adhesion.","abstract_html":"The aim of the research presented in this thesis was to investigate the cell biology behind the settlement and adhesion processes of biofouling algae. Using the fluorescent dye FM 1-43 in Ulva zoospores to follow membrane recycling, rapid mass membrane retrieval of FM 1-43-labelled plasma membrane was found to occur to an endosomal compartment during settlement. Biolistic delivery of dextran Oregon Green BAPTA-1 and Texas Red enabled ratiometric imaging with a 5-fold greater response to Ca<span class=\"etd-inline-math\"><sup>2+</sup></span>-ionophores than AM-ester Ca<span class=\"etd-inline-math\"><sup>2+</sup></span> indicators. During settlement, zoospores exhibited both localised and diffuse increases in cytosolic calcium implying a role in secretion of the adhesive. Secretion of redox-active substrates was detected using amperometry when settled spores were mechano-stimulated. Secretory events were similar to those seen in bovine chromaffin cells with the presence of foot signals in the recordings implying a role for a ‘fusion pore’ in exocytosis. Using DAF-FM DA nitric oxide (NO) production in Seminavis robusta was found to be 4-fold greater on a surface to which the cells adhered weakly than on a surface to which they attached more strongly. Increased NO reduced attachment strength and it is thought that NO may play a signalling and/or regulatory role in diatom adhesion.","abstract_has_math":true,"creators":["Thompson, Stephanie Eleanor Mary"],"institution":"University of Birmingham","degree_name":"d_ph","degree_level":"d_ph","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008-07","date_published":"2008-07","updated_at":"2026-07-24T01:10:46Z","subjects":["QH301 Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://etheses.bham.ac.uk//id/eprint/194/2/Decl_IS_Thompson08PhD.jpg","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.sponsor","label":"Sponsor","values":["bbsrc"]},{"key":"dc:creator","label":"Author","values":["Thompson, Stephanie Eleanor Mary"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2008-07"]},{"key":"dc:date.issued","label":"Date","values":["2008-07"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["School of Biosciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Birmingham"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["http://etheses.bham.ac.uk//id/eprint/194/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["d_ph"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["d_ph"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["QH301 Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://etheses.bham.ac.uk//id/eprint/194/1/Thompson08PhD.pdf","http://etheses.bham.ac.uk//id/eprint/194/2/Decl_IS_Thompson08PhD.jpg"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The aim of the research presented in this thesis was to investigate the cell biology behind the settlement and adhesion processes of biofouling algae. Using the fluorescent dye FM 1-43 in Ulva zoospores to follow membrane recycling, rapid mass membrane retrieval of FM 1-43-labelled plasma membrane was found to occur to an endosomal compartment during settlement. Biolistic delivery of dextran Oregon Green BAPTA-1 and Texas Red enabled ratiometric imaging with a 5-fold greater response to Ca\\(^{2+}\\)-ionophores than AM-ester Ca\\(^{2+}\\) indicators. During settlement, zoospores exhibited both localised and diffuse increases in cytosolic calcium implying a role in secretion of the adhesive. Secretion of redox-active substrates was detected using amperometry when settled spores were mechano-stimulated. Secretory events were similar to those seen in bovine chromaffin cells with the presence of foot signals in the recordings implying a role for a ‘fusion pore’ in exocytosis. Using DAF-FM DA nitric oxide (NO) production in Seminavis robusta was found to be 4-fold greater on a surface to which the cells adhered weakly than on a surface to which they attached more strongly. Increased NO reduced attachment strength and it is thought that NO may play a signalling and/or regulatory role in diatom adhesion."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","image/jpeg"]},{"key":"dc:title","label":"Title","values":["Cell biology of settlement and adhesion processes of biofouling algae"]}]}],"canonical_facts":{"dc:contributor.sponsor":["bbsrc"],"dc:creator":["Thompson, Stephanie Eleanor Mary"],"dc:date":["2008-07"],"dc:date.issued":["2008-07"],"dc:description.abstract":["The aim of the research presented in this thesis was to investigate the cell biology behind the settlement and adhesion processes of biofouling algae. Using the fluorescent dye FM 1-43 in Ulva zoospores to follow membrane recycling, rapid mass membrane retrieval of FM 1-43-labelled plasma membrane was found to occur to an endosomal compartment during settlement. Biolistic delivery of dextran Oregon Green BAPTA-1 and Texas Red enabled ratiometric imaging with a 5-fold greater response to Ca\\(^{2+}\\)-ionophores than AM-ester Ca\\(^{2+}\\) indicators. During settlement, zoospores exhibited both localised and diffuse increases in cytosolic calcium implying a role in secretion of the adhesive. Secretion of redox-active substrates was detected using amperometry when settled spores were mechano-stimulated. Secretory events were similar to those seen in bovine chromaffin cells with the presence of foot signals in the recordings implying a role for a ‘fusion pore’ in exocytosis. Using DAF-FM DA nitric oxide (NO) production in Seminavis robusta was found to be 4-fold greater on a surface to which the cells adhered weakly than on a surface to which they attached more strongly. Increased NO reduced attachment strength and it is thought that NO may play a signalling and/or regulatory role in diatom adhesion."],"dc:format":["application/pdf","image/jpeg"],"dc:identifier.uri":["http://etheses.bham.ac.uk//id/eprint/194/1/Thompson08PhD.pdf","http://etheses.bham.ac.uk//id/eprint/194/2/Decl_IS_Thompson08PhD.jpg"],"dc:publisher.department":["School of Biosciences"],"dc:publisher.institution":["University of Birmingham"],"dc:relation.isreferencedby":["http://etheses.bham.ac.uk//id/eprint/194/"],"dc:subject":["QH301 Biology"],"dc:title":["Cell biology of settlement and adhesion processes of biofouling algae"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["d_ph"],"dc:type.qualificationname":["d_ph"]},"updated_at":"2026-07-24T01:10:46Z"}