{"id":{"repo_id":"baylor","oai_identifier":"oai:baylor-ir.tdl.org:2104/8738"},"canonical_url":"https://search.dev.ndltd.org/etd/baylor/oai:baylor-ir.tdl.org:2104/8738","repository":{"repo_id":"baylor","name":"Baylor University","base_url":"https://baylor-ir.tdl.org/server/oai/request"},"display":{"title":"Copper ionophores induce autophagy in melanoma cells.","abstract":"Malignant melanoma is a particularly aggressive form of skin cancer, for which the only current treatment option is excision. As melanoma is an inherently antiapoptotic model of cancer, other avenues of cell death were pursued, namely autophagy, a mechanism in which a cell devours itself in times of stress. In this study, two copper ionophores, disulfiram and thiomaltol, were investigated against melanoma. By using immunocytochemistry, Western Blotting and supravital staining, it was confirmed that both ionophores induce autophagy, and furthermore, drive the process to completion. In addition, when tested for viability with the autophagy inhibitor hydroxychloroquine, both ionophores showed evidence of synergism. To further study the migration of copper, STEM-EDX was used on thiomaltol treated melanoma cells.","abstract_html":"Malignant melanoma is a particularly aggressive form of skin cancer, for which the only current treatment option is excision. As melanoma is an inherently antiapoptotic model of cancer, other avenues of cell death were pursued, namely autophagy, a mechanism in which a cell devours itself in times of stress. In this study, two copper ionophores, disulfiram and thiomaltol, were investigated against melanoma. By using immunocytochemistry, Western Blotting and supravital staining, it was confirmed that both ionophores induce autophagy, and furthermore, drive the process to completion. In addition, when tested for viability with the autophagy inhibitor hydroxychloroquine, both ionophores showed evidence of synergism. To further study the migration of copper, STEM-EDX was used on thiomaltol treated melanoma cells.","abstract_has_math":false,"creators":["Dao, Long, 1988-"],"institution":"Baylor University.","degree_name":"M.S.","degree_level":"Masters","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Farmer, Patrick Joseph, 1957-"],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-09","date_published":"2013-09","updated_at":"2026-07-24T01:08:13Z","subjects":["Copper ionophores.","Autophagy.","Melanoma.","Disulfiram.","Thiomaltol."],"languages":["en"],"rights":["Baylor University works are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 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By using immunocytochemistry, Western Blotting and supravital staining, it was confirmed that both ionophores induce autophagy, and furthermore, drive the process to completion. In addition, when tested for viability with the autophagy inhibitor hydroxychloroquine, both ionophores showed evidence of synergism. To further study the migration of copper, STEM-EDX was used on thiomaltol treated melanoma cells."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Copper ionophores induce autophagy in melanoma cells."]}]}],"canonical_facts":{"dc:contributor.advisor":["Farmer, Patrick Joseph, 1957-"],"dc:creator":["Dao, Long, 1988-"],"dc:date.accessioned":["2013-09-16T12:59:17Z"],"dc:date.available":["2013-09-16T12:59:17Z"],"dc:date.issued":["2013-09"],"dc:description.abstract":["Malignant melanoma is a particularly aggressive form of skin cancer, for which the only current treatment option is excision. As melanoma is an inherently antiapoptotic model of cancer, other avenues of cell death were pursued, namely autophagy, a mechanism in which a cell devours itself in times of stress. In this study, two copper ionophores, disulfiram and thiomaltol, were investigated against melanoma. By using immunocytochemistry, Western Blotting and supravital staining, it was confirmed that both ionophores induce autophagy, and furthermore, drive the process to completion. In addition, when tested for viability with the autophagy inhibitor hydroxychloroquine, both ionophores showed evidence of synergism. To further study the migration of copper, STEM-EDX was used on thiomaltol treated melanoma cells."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/2104/8738"],"dc:language.iso":["en"],"dc:rights":["Baylor University works are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission."],"dc:subject":["Copper ionophores.","Autophagy.","Melanoma.","Disulfiram.","Thiomaltol."],"dc:title":["Copper ionophores induce autophagy in melanoma cells."],"dc:type":["Thesis"],"thesis:degree_level":["Masters"],"thesis:degree_name":["M.S."],"thesis:institution_name":["Baylor University."]},"updated_at":"2026-07-24T01:08:13Z"}