Uptake and utilization of alanine-1-14C by freshwater algae.

Experiments were conducted x^ith Chlorella pyrenoidosa, Cosmarium formosulum, and Gloeocapsa sp. to determine their relative heterotrophic capacity at various conditions. Cultures were incubated in nutrient media with 0.5 uc DL-alanine-l-^C added at various temperature (10C, 20C, 30C) and illumination (0 ft-c, 2137 ft-c, 8547 ft-c) conditions. A temperature increase resulted in an increase in alanine uptake in Ck pyrenoidosa and C. formosulum. Uptake decreased in Gloeocapsa at 20C but increased at 30C. High light intensity and darkness stimulated uptake in C. pyrenoidosa and C. formosulum and uptake was minimal at 2137 ft-c. Uptake occurred at all illumination conditions with Gloeocapsa, but was maximum at 2137 ft-c. Alanine was incorporated into cytoplasmic protein in C. pyrenoidosa and C.. formosulum but a significant amount was incorporated into protein in Gloeocapsa only at 2137 ft-c. These species were concluded to be facultative heterotrophs.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Masters

Grantor

Baylor University.

Year dc:date.issued

1969

Author dc:creator

• Campbell, David Bennett, 1945-

Advisor dc:contributor.advisor

• Lind, Owen T., 1934-

• Protein.
• Heterotrophs.
• Alanine.

Statement dc:rights

• Baylor University works are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/2104/11671

OAI identifier oai:identifier

oai:baylor-ir.tdl.org:2104/11671