The modification of Anthocyanin biosynthesis in dicotyledonous plants
type: CreativeWork
description: The aim of this study was to modify the pattern of anthocyanin biosynthesis in dicotyledonous plants, in particular legumes, using four different anthocyanin regulatory genes and two promoters. The anthocyanin regulatory genes consisted of a matched pair from maize and a mismatched pair from two dicotyledonous species of plants. In order to accomplish this aim, a series of vectors were made that enabled expression of the genes following both transient and stable transformation experiments in plants. A novel method was devised to overcome the problems associated with the insertion of multiple chimeric genes into a binary transformation vector. A total of fourteen intermediate expression plasmids and sbrteen transformation plasmids were produced. Microparticle bombardment of monocotyledonous and dicotyledonous cell suspensions with constructs made for transient expression, confirmed that the coding regions of various constructs were functional. Microparticle bombardment with the maize anthocyanin regulatory genes stimulated anthocyanin accumulation when a member from each myc-type and myb-typc family was delivered with a the particle inflow gun (PIG) into monocotyledonous cell lines. Microparticle bombardment of dicotyledonous ceU suspensions with the maize anthocyanin regulatory genes did not stimulate anthocyanin accumulation. The efficiencies of microparticle bombardment with two types of microparticle delivery systems, the BioRad and PIG, were compared and it was determined that the BioRad helium gun was more proficient than the PIG for transient anthocyanin production in a monocotyledonous ceU suspension. Microparticle bombardment of maize suspension cultures with the maize anthocyanin regulatory genes using the BioRad helium gun, provided an understanding of the stoichiometry between the two families of regulatory genes. Delivering maize anthocyanin regulatory genes into a cell suspension of Trifolium subterraneum with the BioRad helium gun induced anthocyanin a…
abstract: The aim of this study was to modify the pattern of anthocyanin biosynthesis in dicotyledonous plants, in particular legumes, using four different anthocyanin regulatory genes and two promoters. The anthocyanin regulatory genes consisted of a matched pair from maize and a mismatched pair from two dicotyledonous species of plants. In order to accomplish this aim, a series of vectors were made that enabled expression of the genes following both transient and stable transformation experiments in plants. A novel method was devised to overcome the problems associated with the insertion of multiple chimeric genes into a binary transformation vector. A total of fourteen intermediate expression plasmids and sbrteen transformation plasmids were produced. Microparticle bombardment of monocotyledonous and dicotyledonous cell suspensions with constructs made for transient expression, confirmed that the coding regions of various constructs were functional. Microparticle bombardment with the maize anthocyanin regulatory genes stimulated anthocyanin accumulation when a member from each myc-type and myb-typc family was delivered with a the particle inflow gun (PIG) into monocotyledonous cell lines. Microparticle bombardment of dicotyledonous ceU suspensions with the maize anthocyanin regulatory genes did not stimulate anthocyanin accumulation. The efficiencies of microparticle bombardment with two types of microparticle delivery systems, the BioRad and PIG, were compared and it was determined that the BioRad helium gun was more proficient than the PIG for transient anthocyanin production in a monocotyledonous ceU suspension. Microparticle bombardment of maize suspension cultures with the maize anthocyanin regulatory genes using the BioRad helium gun, provided an understanding of the stoichiometry between the two families of regulatory genes. Delivering maize anthocyanin regulatory genes into a cell suspension of Trifolium subterraneum with the BioRad helium gun induced anthocyanin a…
author: De Majnik, John Paul
language: en
date published: 1997
date modified: 2026-07-24T01:07:43Z
provider: Australian Catholic University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Australian Catholic University

PropertyValue
property ID: Handle
value: http://hdl.handle.net/1885/109571