The metabolism of cortisol in the human feto-placental unit
type: CreativeWork
description: A method for assaying cortisol and cortisone using chromatography on either Paper or Sephadex LH-20 columns, followed by competitive protein binding, has been applied to umbilical cord and maternal plasma samples. In mixed cord plasma the mean cortisol concentration was 6.0 ± 0.8 µg/100 ml (n = 9) and the mean cortisone concentration was 13.5 ± 2.9 µg/100 ml (n = 9). In cord arterial plasma the mean cortisol concentration was 6.3 ± 2.9 µg/100 ml (n = 6). and the mean cortisone level was 10.1 ± 2.5 µg/100 ml (n = 6). For cord venous plasma, the mean level of cortisol was 5.6 ± 1.5 µg/100 ml (n = 6) and of cortisone was 13.5 ± 2.4 µg/100 ml (n = 6). Maternal plasma gave a mean value of cortisol of 42.3 ± 4.5 µg/100 ml (n = 6) and of cortisone of 6.2 ± 0.9 µg/100 ml (n = 6). The mean value of cortisol in mixed cord plasma of live anencephalic fetuses was 3.3 ± 1.8 µg/100 ml (n = 3) and of cortisone was 13.6 ± 3.2 µg/100 ml (n = 3). Further assays employing a radioimmunoassay for cortisol, and competitive protein binding for cortisone have been applied to cord plasma samples of prematurely-delivered fetuses, after treatment of betamethasone to the mother (12 mg/day for one or two days). The mean value of cortisol in samples from fetuses delivered up to 24 hours after treatment was 2.6 ± 2.2 µg/100 ml (n = 4) and the mean value of cortisone was 2.2 ± 2.2 µg/100 ml (n = 14). For those fetuses delivered over 72 hours after betamethasone treatment, mean levels of cortisol and cortisone were respectively 10.4 ± 3.2 µg/100 ml and 9.4 ± 3.4 µg/100 ml (n = 3) compared to control values without betamethasone treatment of 8.2 ± 3.5 µg/1 0 ml and 9.6 ± 3.7 µg/100 ml (n = 3) respectively. Kinetic parameters of the human placental 11β-hydroxysteroid dehydrogenase system were assessed using a microsomal preparation in 50% glycerol buffer. The Michaelis constant for cortisol was 1.88 x 10-6M, in the presence of NADP, and 0.56 x 10-6M, in the presence of NAD. Vmax values varied with co…
abstract: A method for assaying cortisol and cortisone using chromatography on either Paper or Sephadex LH-20 columns, followed by competitive protein binding, has been applied to umbilical cord and maternal plasma samples. In mixed cord plasma the mean cortisol concentration was 6.0 ± 0.8 µg/100 ml (n = 9) and the mean cortisone concentration was 13.5 ± 2.9 µg/100 ml (n = 9). In cord arterial plasma the mean cortisol concentration was 6.3 ± 2.9 µg/100 ml (n = 6). and the mean cortisone level was 10.1 ± 2.5 µg/100 ml (n = 6). For cord venous plasma, the mean level of cortisol was 5.6 ± 1.5 µg/100 ml (n = 6) and of cortisone was 13.5 ± 2.4 µg/100 ml (n = 6). Maternal plasma gave a mean value of cortisol of 42.3 ± 4.5 µg/100 ml (n = 6) and of cortisone of 6.2 ± 0.9 µg/100 ml (n = 6). The mean value of cortisol in mixed cord plasma of live anencephalic fetuses was 3.3 ± 1.8 µg/100 ml (n = 3) and of cortisone was 13.6 ± 3.2 µg/100 ml (n = 3). Further assays employing a radioimmunoassay for cortisol, and competitive protein binding for cortisone have been applied to cord plasma samples of prematurely-delivered fetuses, after treatment of betamethasone to the mother (12 mg/day for one or two days). The mean value of cortisol in samples from fetuses delivered up to 24 hours after treatment was 2.6 ± 2.2 µg/100 ml (n = 4) and the mean value of cortisone was 2.2 ± 2.2 µg/100 ml (n = 14). For those fetuses delivered over 72 hours after betamethasone treatment, mean levels of cortisol and cortisone were respectively 10.4 ± 3.2 µg/100 ml and 9.4 ± 3.4 µg/100 ml (n = 3) compared to control values without betamethasone treatment of 8.2 ± 3.5 µg/1 0 ml and 9.6 ± 3.7 µg/100 ml (n = 3) respectively. Kinetic parameters of the human placental 11β-hydroxysteroid dehydrogenase system were assessed using a microsomal preparation in 50% glycerol buffer. The Michaelis constant for cortisol was 1.88 x 10-6M, in the presence of NADP, and 0.56 x 10-6M, in the presence of NAD. Vmax values varied with co…
author: Dormer, Robyn Alethea
language: en
license: https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
date published: 1976
date modified: 2026-07-24T01:04:52Z
provider: University of Auckland
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: ResearchSpace@Auckland

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property ID: Handle
value: https://hdl.handle.net/2292/3094