Characterisation and detection of viruses (Cucumovirus, Potyvirus) infecting vanilla in Réunion Island and Polynesian Islands
type: CreativeWork
description: Natural vanilla (Vanilla planifolia, V. tahitensis) is economically important for a number of producing countries, but viral diseases are prejudicial to its successful cultivation. Techniques are required to detect viruses in vanilla plants in order to establish quarantine procedures and sources of virus-free planting material. This study contributed to the progress in managing viral diseases of vanilla by firstly identifying Cucumber mosaic virus (CMV, cucumovirus) as causing severe distortion, stunting, sterility and sometimes death of vanilla plants. Vanilla CMV isolates from French Polynesia (Pacific Ocean) and Réunion Island (Indian Ocean) were classified in CMV subgroup IB, adding to the short list of subgroup IB isolates detected outside of Asia. Two isolates were putatively classified in subgroup IA, suggesting that CMV in vanilla is still evolving and/or that vanilla is infected from different sources. Subgroup II CMV from New Zealand was also experimentally infectious to V. planifolia, showing that vanilla crops should be protected from all potential sources of CMV inoculum. Existing serological and molecular detection rests were performant for the detection of CMV directly from vanilla tissue. Secondly, this study provided the first coat protein sequence information for Vanilla mosaic virus (VanMV, Potyvirus). A Cook Islands isolate (VanMV-CI) and a French Polynesia isolate (VanMV-FP) had distinctly different coat proteins. The VanMV-FP CP N-terminus contained a stretch of amino-acid repeats (GTN) typical of natively unfolded proteins. This GTN stretch was located downstream of a DVG motif (which replaced the more common aphid transmission DAG motif), suggesting a role in improving aphid transmission, or regulating formation of the HC-virus complex. CP core nucleotide sequence identities indicated VanMV-CI and VanMV-FP were strains of Dasheen mosaic virus (DsMV). In contrast, CP amino-acid sequence homologies between VanMV- CI and DsMV were intermediate b…
abstract: Natural vanilla (Vanilla planifolia, V. tahitensis) is economically important for a number of producing countries, but viral diseases are prejudicial to its successful cultivation. Techniques are required to detect viruses in vanilla plants in order to establish quarantine procedures and sources of virus-free planting material. This study contributed to the progress in managing viral diseases of vanilla by firstly identifying Cucumber mosaic virus (CMV, cucumovirus) as causing severe distortion, stunting, sterility and sometimes death of vanilla plants. Vanilla CMV isolates from French Polynesia (Pacific Ocean) and Réunion Island (Indian Ocean) were classified in CMV subgroup IB, adding to the short list of subgroup IB isolates detected outside of Asia. Two isolates were putatively classified in subgroup IA, suggesting that CMV in vanilla is still evolving and/or that vanilla is infected from different sources. Subgroup II CMV from New Zealand was also experimentally infectious to V. planifolia, showing that vanilla crops should be protected from all potential sources of CMV inoculum. Existing serological and molecular detection rests were performant for the detection of CMV directly from vanilla tissue. Secondly, this study provided the first coat protein sequence information for Vanilla mosaic virus (VanMV, Potyvirus). A Cook Islands isolate (VanMV-CI) and a French Polynesia isolate (VanMV-FP) had distinctly different coat proteins. The VanMV-FP CP N-terminus contained a stretch of amino-acid repeats (GTN) typical of natively unfolded proteins. This GTN stretch was located downstream of a DVG motif (which replaced the more common aphid transmission DAG motif), suggesting a role in improving aphid transmission, or regulating formation of the HC-virus complex. CP core nucleotide sequence identities indicated VanMV-CI and VanMV-FP were strains of Dasheen mosaic virus (DsMV). In contrast, CP amino-acid sequence homologies between VanMV- CI and DsMV were intermediate b…
author: Le Roux, Karin, 1974-
language: en
license: https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm
identifier: THESIS 05-226
date published: 2005
date modified: 2026-07-24T01:04:34Z
provider: University of Auckland
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: ResearchSpace@Auckland

PropertyValue
property ID: Handle
value: https://hdl.handle.net/2292/1289