A technique for the measurement of biopterin using HPLC has been developed and compared against the established Crithidia fasciculata assay. The two assays give a similar reading when samples are unoxidised but after acid/iodine oxidation Crithidia reads higher, probably due to the presence of other pterins. Using HPLC as a measuring technique an oral dose of phenylalanine has been found to elevate the level of biopterin derivatives in the blood whilst tyrosine had no effect...
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