{"id":{"repo_id":"aston","oai_identifier":"oai:publications.aston.ac.uk:11550"},"canonical_url":"https://search.dev.ndltd.org/etd/aston/oai:publications.aston.ac.uk:11550","repository":{"repo_id":"aston","name":"Aston University","base_url":"https://publications.aston.ac.uk/cgi/oai2"},"display":{"title":"The Fate of Folic Acid and Related Compounds in the Rat","abstract":"The physiological disposition and metabolism of : folates was studied by purification, characterisation and specific radioactivity determinations of compounds derived from oral doses of labelled folates. At doses of 22 and 320ug of pteroyl-L-glutamic acid/ kg, 50 and 25% respectively was absorbed, With doses of 320ug of [214-C]pteroyl-L-glutamic acid/kg, radioactivity was detected in the peripheral circulation 10 minutes after administration. Pteroyl-L-glutemic acid was absorbed without metabolism and was present in systemic blood for up to 4 hours after administration. With doses of pteroyl-L-glutamic acid from 3.1 to 56ug/kg approximately 6% of the dose was excreted in 24 hours; whilst at 320ug/kg 30% was excreted mainly in the 2 to 4 hour period. Three urinary folates were isolated. One sf the major metabolites was 5-methyltetrahydro-pteroylglutamic acid. The others were unidentified but were not pteroylglutamic acid, 7,8-dihydro-, 5,6,7,8-tetrahydro-, 5- or 10-formyltetrahydro-, 5,10~methylidyne-tetrahydro-, 5,formimidoyltetrahydro-, 5,10-methylene-tetrahydro-, 5-methyltetrahydro-pteroylglutamic acid, nor any decomposition products of these compounds formed during isolation. Labelled unconjugated pteridines were absent. Six hours after administration of pteroyl-L-glutamic acid, more than seven labelled compounds were isolated from liver homogenates. Extractions performed up to 10 days after administration or after prior starvation only conjugates, 5-methyltetrahydro~pteroylglutamate was the major monoglutamate and represented 10% of the total hepatic folates. The physiological disposition of the natural diastereoisomer of 5-methyltetrahydro-pteroylglutamate was similar to that of pteroylglutamate. At a dose of 84u¢/keg, 80% of the urinary folates was 5-methyltetrahydropteroylglutamate, but at lower doses a greater proportion was present as metabolites. The major unidentified metabolite excreted after pteroylglutamic acid administration showed a similar absorption and excretion pattern to 5-methyltetrahydropteroylglutamic acid and was metabolised to 5-methyltetrahydro-pteroylgiutamate. The results are discussed with reference to the nutritional, metabolic and clinical importance of folates in previous work.","abstract_html":"The physiological disposition and metabolism of : folates was studied by purification, characterisation and specific radioactivity determinations of compounds derived from oral doses of labelled folates. At doses of 22 and 320ug of pteroyl-L-glutamic acid/ kg, 50 and 25% respectively was absorbed, With doses of 320ug of [214-C]pteroyl-L-glutamic acid/kg, radioactivity was detected in the peripheral circulation 10 minutes after administration. Pteroyl-L-glutemic acid was absorbed without metabolism and was present in systemic blood for up to 4 hours after administration. With doses of pteroyl-L-glutamic acid from 3.1 to 56ug/kg approximately 6% of the dose was excreted in 24 hours; whilst at 320ug/kg 30% was excreted mainly in the 2 to 4 hour period. Three urinary folates were isolated. One sf the major metabolites was 5-methyltetrahydro-pteroylglutamic acid. The others were unidentified but were not pteroylglutamic acid, 7,8-dihydro-, 5,6,7,8-tetrahydro-, 5- or 10-formyltetrahydro-, 5,10~methylidyne-tetrahydro-, 5,formimidoyltetrahydro-, 5,10-methylene-tetrahydro-, 5-methyltetrahydro-pteroylglutamic acid, nor any decomposition products of these compounds formed during isolation. Labelled unconjugated pteridines were absent. Six hours after administration of pteroyl-L-glutamic acid, more than seven labelled compounds were isolated from liver homogenates. Extractions performed up to 10 days after administration or after prior starvation only conjugates, 5-methyltetrahydro~pteroylglutamate was the major monoglutamate and represented 10% of the total hepatic folates. The physiological disposition of the natural diastereoisomer of 5-methyltetrahydro-pteroylglutamate was similar to that of pteroylglutamate. At a dose of 84u¢/keg, 80% of the urinary folates was 5-methyltetrahydropteroylglutamate, but at lower doses a greater proportion was present as metabolites. The major unidentified metabolite excreted after pteroylglutamic acid administration showed a similar absorption and excretion pattern to 5-methyltetrahydropteroylglutamic acid and was metabolised to 5-methyltetrahydro-pteroylgiutamate. The results are discussed with reference to the nutritional, metabolic and clinical importance of folates in previous work.","abstract_has_math":false,"creators":["Dransfield, Eric"],"institution":"Aston University","degree_name":"Ph.D.","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1972,"date_issued":"1972","date_published":"1972","updated_at":"2026-07-24T01:01:30Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Dransfield, Eric"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1972"]},{"key":"dc:date.issued","label":"Date","values":["1972"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Chemical Engineering & Applied Chemistry"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Aston University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://publications.aston.ac.uk/id/eprint/11550/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Ph.D."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://publications.aston.ac.uk/id/eprint/11550/1/Dransfield_1972_reduced.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The physiological disposition and metabolism of : folates was studied by purification, characterisation and specific radioactivity determinations of compounds derived from oral doses of labelled folates. At doses of 22 and 320ug of pteroyl-L-glutamic acid/ kg, 50 and 25% respectively was absorbed, With doses of 320ug of [214-C]pteroyl-L-glutamic acid/kg, radioactivity was detected in the peripheral circulation 10 minutes after administration. Pteroyl-L-glutemic acid was absorbed without metabolism and was present in systemic blood for up to 4 hours after administration. With doses of pteroyl-L-glutamic acid from 3.1 to 56ug/kg approximately 6% of the dose was excreted in 24 hours; whilst at 320ug/kg 30% was excreted mainly in the 2 to 4 hour period. Three urinary folates were isolated. One sf the major metabolites was 5-methyltetrahydro-pteroylglutamic acid. The others were unidentified but were not pteroylglutamic acid, 7,8-dihydro-, 5,6,7,8-tetrahydro-, 5- or 10-formyltetrahydro-, 5,10~methylidyne-tetrahydro-, 5,formimidoyltetrahydro-, 5,10-methylene-tetrahydro-, 5-methyltetrahydro-pteroylglutamic acid, nor any decomposition products of these compounds formed during isolation. Labelled unconjugated pteridines were absent. Six hours after administration of pteroyl-L-glutamic acid, more than seven labelled compounds were isolated from liver homogenates. Extractions performed up to 10 days after administration or after prior starvation only conjugates, 5-methyltetrahydro~pteroylglutamate was the major monoglutamate and represented 10% of the total hepatic folates. The physiological disposition of the natural diastereoisomer of 5-methyltetrahydro-pteroylglutamate was similar to that of pteroylglutamate. At a dose of 84u¢/keg, 80% of the urinary folates was 5-methyltetrahydropteroylglutamate, but at lower doses a greater proportion was present as metabolites. The major unidentified metabolite excreted after pteroylglutamic acid administration showed a similar absorption and excretion pattern to 5-methyltetrahydropteroylglutamic acid and was metabolised to 5-methyltetrahydro-pteroylgiutamate. The results are discussed with reference to the nutritional, metabolic and clinical importance of folates in previous work."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["The Fate of Folic Acid and Related Compounds in the Rat"]}]}],"canonical_facts":{"dc:creator":["Dransfield, Eric"],"dc:date":["1972"],"dc:date.issued":["1972"],"dc:description.abstract":["The physiological disposition and metabolism of : folates was studied by purification, characterisation and specific radioactivity determinations of compounds derived from oral doses of labelled folates. At doses of 22 and 320ug of pteroyl-L-glutamic acid/ kg, 50 and 25% respectively was absorbed, With doses of 320ug of [214-C]pteroyl-L-glutamic acid/kg, radioactivity was detected in the peripheral circulation 10 minutes after administration. Pteroyl-L-glutemic acid was absorbed without metabolism and was present in systemic blood for up to 4 hours after administration. With doses of pteroyl-L-glutamic acid from 3.1 to 56ug/kg approximately 6% of the dose was excreted in 24 hours; whilst at 320ug/kg 30% was excreted mainly in the 2 to 4 hour period. Three urinary folates were isolated. One sf the major metabolites was 5-methyltetrahydro-pteroylglutamic acid. The others were unidentified but were not pteroylglutamic acid, 7,8-dihydro-, 5,6,7,8-tetrahydro-, 5- or 10-formyltetrahydro-, 5,10~methylidyne-tetrahydro-, 5,formimidoyltetrahydro-, 5,10-methylene-tetrahydro-, 5-methyltetrahydro-pteroylglutamic acid, nor any decomposition products of these compounds formed during isolation. Labelled unconjugated pteridines were absent. Six hours after administration of pteroyl-L-glutamic acid, more than seven labelled compounds were isolated from liver homogenates. Extractions performed up to 10 days after administration or after prior starvation only conjugates, 5-methyltetrahydro~pteroylglutamate was the major monoglutamate and represented 10% of the total hepatic folates. The physiological disposition of the natural diastereoisomer of 5-methyltetrahydro-pteroylglutamate was similar to that of pteroylglutamate. At a dose of 84u¢/keg, 80% of the urinary folates was 5-methyltetrahydropteroylglutamate, but at lower doses a greater proportion was present as metabolites. The major unidentified metabolite excreted after pteroylglutamic acid administration showed a similar absorption and excretion pattern to 5-methyltetrahydropteroylglutamic acid and was metabolised to 5-methyltetrahydro-pteroylgiutamate. The results are discussed with reference to the nutritional, metabolic and clinical importance of folates in previous work."],"dc:format":["text"],"dc:identifier.uri":["https://publications.aston.ac.uk/id/eprint/11550/1/Dransfield_1972_reduced.pdf"],"dc:publisher.department":["Chemical Engineering & Applied Chemistry"],"dc:publisher.institution":["Aston University"],"dc:relation.isreferencedby":["https://publications.aston.ac.uk/id/eprint/11550/"],"dc:title":["The Fate of Folic Acid and Related Compounds in the Rat"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["Ph.D."]},"updated_at":"2026-07-24T01:01:30Z"}