Field Amplified Sample Stacking on Amyloid Beta (1-42) Oligomers using Capillary Electrophoresis

<p>According to recent literature, it is believed that the oligomeric form of amyloid beta (Aβ) is the leading cause of Alzheimer’s disease (1; 8; 10; 12-18). Additionally, recent studies have eluded to the impact of Alzheimer’s disease (AD) both economically and Socially in today’s society where an increase of about 71% of AD related deaths were recorded between 2000 and 2013 (7). Since the oligomeric forms of Aβ vary in size, shape and some believe conformation, it is vital to utilize a separation technique, such as capilllary electrophoresis (CE) to further understand Aβ aggregation. By understanding Aβ aggregation, treatment of AD or preventive care measurements could be additionally developed.</p> <p>Therefore in this study, field amplified sample stacking (FASS) technique on the CE was utilized to provide higher resolution in oligomeric Aβ1-42 detection without causing significant changes to the aggregation. It was observed that the FASS technique provided smaller peak widths and increased peak heights on the CE compared to the non-FASS conditions. Furthermore by conducting thioflavin-t (ThT) assays, it was observed changing the buffer concentrations in accordance to the FASS technique conditions did not effect the overall aggregation. Thioflavin-t (ThT) assays were also conducted in order to determine an agitation rate where the oligomers of Aβ1-42 were observed on the CE.</p> <p>The oligomeric species observed were believed to be less than or equal to 100 kDa. Additionally, Congo red and Orange G inhibition were conducted to confirm oligomeric Aβ1-42 species were observed on the CE. Both inhibition studies alongside TEM imaging proved the aggregates observed on the CE in the 27 hour aggregation were smaller than proto-fibrils. Future work on natural compound inhibition studies using CE are recommended to see how those inhibitors target Aβ1-42 species that are less than or equal to 100 kDa.</p>

Name thesis:degree_name

Master of Science in Chemical Engineering (MSChE)

Level thesis:degree_level

Thesis

Year dc:date.available

2015

Author dc:creator

• Paracha, Sadia Ali

Advisor dc:contributor.advisor

• Hestekin, Christa N.

Contributors dc:contributor

• Beitle, Robert R. Jr.
• Sakon, Joshua

• Applied sciences
• Alzheimer's disease
• Amyloid beta
• Capillary electrophoresis
• Fass technique
• Oligomers
• Other Chemical Engineering

Repository record dc:identifier

https://scholarworks.uark.edu/etd/1388

OAI identifier oai:identifier

oai:scholarworks.uark.edu:etd-2387