Histological Changes in Photorejuvenation Induced by 5-Aminolevulinic Acid Photodynamic Therapy in a Photoaged Mouse Model
type: CreativeWork
description: Background: Photoaging is defined as the premature skin aging induced by ultraviolet (UV) irradiation, which is histologivally characterized by degradation of collagen fiber and accumulation of abnormal elastotic material in the dermis. Photodynamic therapy (PDT) is a noninvasive technique used in the treatment of various skin disorders, and recently it has been shown to be effective for photoaged skins. However, the histological studies of PDT in animal model have rarely been reported. Objectives: I observed the histological changes in photoaged mouse skin specimens and in the specimens after photorejuvenation induced by 5-aminolevulinic acid (ALA)-PDT. Furthermore, I investigated transmission electron microscopic (TEM) studies to observe the differences among normal, photoaged, and post-ALA-PDT biopsy specimens. Materials and Methods: Total 32 of 6-week-old female albino hairless mice (Skh:hr-1) were divided into six groups: control group (group A), photoaging group (group B), ALA only group (group C), light-emitting diode (LED) only group (group D), 5-ALA PDT group with 20J/cm2 of light dose (group E), and 5-ALA PDT group with 40J/cm2 of light dose (group F). For group E, it was divided to 3 identical groups; which were performed from 1 session to 3 sessions of ALA-PDT to investigate the differences among the number of total sessions of PDT. Serial skin biopsies were performed from each mouse at day 2, day 7, day 14, and day 21 from each intervention. For group E, the additional skin biopsies were performed at day 28 from each session. Hematoxylin & Eosin staining, Masson-trichrome staining, and Verhoeff’s elastic staining were performed with additional immunohistochemical staining. Furthermore, TEM studies performed. Results: During photoaging, both epidermal thickness and dermal thickness were increased, the amount of dermal collagen fiber was decreased, and the elastotic materials were increased. After ALA-PDT application, both epidermal thickness and dermal t…
abstract: Background: Photoaging is defined as the premature skin aging induced by ultraviolet (UV) irradiation, which is histologivally characterized by degradation of collagen fiber and accumulation of abnormal elastotic material in the dermis. Photodynamic therapy (PDT) is a noninvasive technique used in the treatment of various skin disorders, and recently it has been shown to be effective for photoaged skins. However, the histological studies of PDT in animal model have rarely been reported. Objectives: I observed the histological changes in photoaged mouse skin specimens and in the specimens after photorejuvenation induced by 5-aminolevulinic acid (ALA)-PDT. Furthermore, I investigated transmission electron microscopic (TEM) studies to observe the differences among normal, photoaged, and post-ALA-PDT biopsy specimens. Materials and Methods: Total 32 of 6-week-old female albino hairless mice (Skh:hr-1) were divided into six groups: control group (group A), photoaging group (group B), ALA only group (group C), light-emitting diode (LED) only group (group D), 5-ALA PDT group with 20J/cm2 of light dose (group E), and 5-ALA PDT group with 40J/cm2 of light dose (group F). For group E, it was divided to 3 identical groups; which were performed from 1 session to 3 sessions of ALA-PDT to investigate the differences among the number of total sessions of PDT. Serial skin biopsies were performed from each mouse at day 2, day 7, day 14, and day 21 from each intervention. For group E, the additional skin biopsies were performed at day 28 from each session. Hematoxylin & Eosin staining, Masson-trichrome staining, and Verhoeff’s elastic staining were performed with additional immunohistochemical staining. Furthermore, TEM studies performed. Results: During photoaging, both epidermal thickness and dermal thickness were increased, the amount of dermal collagen fiber was decreased, and the elastotic materials were increased. After ALA-PDT application, both epidermal thickness and dermal t…
author: 박, 지윤
language: en
keywords: photodynamic therapy, photorejuvenation, 5-aminolevulinic acid, mouse model, histological change, transmission electron microscopy
date published: 2012-10-30T04:22:17Z
date modified: 2026-07-24T00:51:39Z
provider: Ajou University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ajou University