{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/7539"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/7539","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"Anti-inflammatory Role of IL-15Rα Up-regulated by Poly I:C in Herpes Simplex Virus-induced Behcet's Disease-like Mouse Model","abstract":"Behcet’s disease (BD) is a chronic, multi-systemic disorder that has arthritic, intestinal, mucocutaneous, ocular, vascular, and central nervous system affects. Interleukin-15 receptor alpha (IL-15Rα) forms stable complex with IL-15 on the cell surface of activated monocytes and mediates the proliferation of memory CD8+ T cells. Recent studies informed that Polyinosinic:polycytidylic acid (Poly I:C) is an immunostimulant which boosts the generation of memory T cells though induction of IL-15 and IL-15Rα. We found that the frequencies of IL-15Rα were significantly lower in HSV-induced BD mouse model than control mice. Therefore, this study was conducted to determine whether Poly I:C supplementation could induce memory cells through up-regulation of IL-15Rα, and reduce inflammation in HSV-induced BD symptoms. To evaluate whether the expression of IL-15Rα could be increased by poly I:C in vivo, Poly I:C was administered into normal and BD mice. Then, Poly I:C administered BD mice were subsequently observed the change of symptoms until 17 days after the last injection. The frequencies of IL-15Rα and memory CD8+ T cells were significantly up-regulated in Poly I:C injected mice. Poly I:C improved the symptoms and decreased severity score through down-regulation of pro-inflammatory cytokines and elevated regulatory and memory T cells. In conclusion, these results suggest that IL-15Rα is an important factor for the regulation of symptoms in HSV-induced BD mice.","abstract_html":"Behcet’s disease (BD) is a chronic, multi-systemic disorder that has arthritic, intestinal, mucocutaneous, ocular, vascular, and central nervous system affects. Interleukin-15 receptor alpha (IL-15Rα) forms stable complex with IL-15 on the cell surface of activated monocytes and mediates the proliferation of memory CD8+ T cells. Recent studies informed that Polyinosinic:polycytidylic acid (Poly I:C) is an immunostimulant which boosts the generation of memory T cells though induction of IL-15 and IL-15Rα. We found that the frequencies of IL-15Rα were significantly lower in HSV-induced BD mouse model than control mice. Therefore, this study was conducted to determine whether Poly I:C supplementation could induce memory cells through up-regulation of IL-15Rα, and reduce inflammation in HSV-induced BD symptoms. To evaluate whether the expression of IL-15Rα could be increased by poly I:C in vivo, Poly I:C was administered into normal and BD mice. Then, Poly I:C administered BD mice were subsequently observed the change of symptoms until 17 days after the last injection. The frequencies of IL-15Rα and memory CD8+ T cells were significantly up-regulated in Poly I:C injected mice. Poly I:C improved the symptoms and decreased severity score through down-regulation of pro-inflammatory cytokines and elevated regulatory and memory T cells. In conclusion, these results suggest that IL-15Rα is an important factor for the regulation of symptoms in HSV-induced BD mice.","abstract_has_math":false,"creators":["최, 주영"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["손, 성향","대학원 의생명과학과","201024353"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-10-26T03:54:39Z","date_published":"2012-10-26T03:54:39Z","updated_at":"2026-07-24T00:51:39Z","subjects":["Behcet's disease","IL-15Rα","Poly I:C","memory CD8+ T cells","Herpes Simplex virus","mouse model"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000012284","000000012284"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000012284","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000012284","code":true},{"text":"000000012284","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/7539","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["손, 성향","대학원 의생명과학과","201024353","최, 주영"]},{"key":"dc:creator","label":"Author","values":["최, 주영"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2012-10-26T03:54:39Z","2012"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Behcet's disease","IL-15Rα","Poly I:C","memory CD8+ T cells","Herpes Simplex virus","mouse model"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/7539","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000012284","000000012284"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Behcet’s disease (BD) is a chronic, multi-systemic disorder that has arthritic, intestinal, mucocutaneous, ocular, vascular, and central nervous system affects. Interleukin-15 receptor alpha (IL-15Rα) forms stable complex with IL-15 on the cell surface of activated monocytes and mediates the proliferation of memory CD8+ T cells. Recent studies informed that Polyinosinic:polycytidylic acid (Poly I:C) is an immunostimulant which boosts the generation of memory T cells though induction of IL-15 and IL-15Rα. We found that the frequencies of IL-15Rα were significantly lower in HSV-induced BD mouse model than control mice. Therefore, this study was conducted to determine whether Poly I:C supplementation could induce memory cells through up-regulation of IL-15Rα, and reduce inflammation in HSV-induced BD symptoms. To evaluate whether the expression of IL-15Rα could be increased by poly I:C in vivo, Poly I:C was administered into normal and BD mice. Then, Poly I:C administered BD mice were subsequently observed the change of symptoms until 17 days after the last injection. The frequencies of IL-15Rα and memory CD8+ T cells were significantly up-regulated in Poly I:C injected mice. Poly I:C improved the symptoms and decreased severity score through down-regulation of pro-inflammatory cytokines and elevated regulatory and memory T cells. In conclusion, these results suggest that IL-15Rα is an important factor for the regulation of symptoms in HSV-induced BD mice.","TABLE OF CONTENTS ABSTRACT ⅰ TABLE OF CONTENTS Ⅲ LIST OF FIGURE ⅴ LIST OF TABLES ⅶ Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 3 A. Animal experiments 3 B. BD symptoms in a mouse model 3 C. In vivo administration of Poly I:C 4 D. Flow cytometric analysis of cell surface staining 4 E. ELISA 5 F. Reverse transcription PCR (RT-PCR) 5 G. Statistical analysis 6 Ⅲ. RESULTS 7 A. The frequencies of IL-15Rα expressing cells in BD mice compared with BDN mice 9 B. Poly I:C up-regulates expression of IL-15Rα in PBMC of normal mice 12 C. Poly I:C ameliorates HSV-induced BD symptoms 16 D. Poly I:C up-regulates the frequencies of IL-15Rα but not IL-7Rα (CD127) in BD mice 18 E. Poly I:C induces CD8+CD44+ memory T cells in BD mice 20 F. Poly I:C up-regulate CD122(IL-15/2Rβ) and CD8+CD122+ T cells in BD mice 24 G. Poly I:C down-regulates IL-23 receptor and IL-17A in BD mice 28 Ⅳ. DISCUSSION 30 Ⅴ. CONCLUSION 34 REFERENCES 36 국문요약 45","Master"]},{"key":"dc:title","label":"Title","values":["Anti-inflammatory Role of IL-15Rα Up-regulated by Poly I:C in Herpes Simplex Virus-induced Behcet's Disease-like Mouse Model","단순포진 바이러스로 유도한 베체트병 마우스모델에서 Poly I:C 에 의해 유도된 IL-15Rα의 항 염증 효과에 관한 연구"]}]}],"canonical_facts":{"dc:contributor":["손, 성향","대학원 의생명과학과","201024353","최, 주영"],"dc:creator":["최, 주영"],"dc:date":["2012-10-26T03:54:39Z","2012"],"dc:description":["Behcet’s disease (BD) is a chronic, multi-systemic disorder that has arthritic, intestinal, mucocutaneous, ocular, vascular, and central nervous system affects. Interleukin-15 receptor alpha (IL-15Rα) forms stable complex with IL-15 on the cell surface of activated monocytes and mediates the proliferation of memory CD8+ T cells. Recent studies informed that Polyinosinic:polycytidylic acid (Poly I:C) is an immunostimulant which boosts the generation of memory T cells though induction of IL-15 and IL-15Rα. We found that the frequencies of IL-15Rα were significantly lower in HSV-induced BD mouse model than control mice. Therefore, this study was conducted to determine whether Poly I:C supplementation could induce memory cells through up-regulation of IL-15Rα, and reduce inflammation in HSV-induced BD symptoms. To evaluate whether the expression of IL-15Rα could be increased by poly I:C in vivo, Poly I:C was administered into normal and BD mice. Then, Poly I:C administered BD mice were subsequently observed the change of symptoms until 17 days after the last injection. The frequencies of IL-15Rα and memory CD8+ T cells were significantly up-regulated in Poly I:C injected mice. Poly I:C improved the symptoms and decreased severity score through down-regulation of pro-inflammatory cytokines and elevated regulatory and memory T cells. In conclusion, these results suggest that IL-15Rα is an important factor for the regulation of symptoms in HSV-induced BD mice.","TABLE OF CONTENTS ABSTRACT ⅰ TABLE OF CONTENTS Ⅲ LIST OF FIGURE ⅴ LIST OF TABLES ⅶ Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 3 A. Animal experiments 3 B. BD symptoms in a mouse model 3 C. In vivo administration of Poly I:C 4 D. Flow cytometric analysis of cell surface staining 4 E. ELISA 5 F. Reverse transcription PCR (RT-PCR) 5 G. Statistical analysis 6 Ⅲ. RESULTS 7 A. The frequencies of IL-15Rα expressing cells in BD mice compared with BDN mice 9 B. Poly I:C up-regulates expression of IL-15Rα in PBMC of normal mice 12 C. Poly I:C ameliorates HSV-induced BD symptoms 16 D. Poly I:C up-regulates the frequencies of IL-15Rα but not IL-7Rα (CD127) in BD mice 18 E. Poly I:C induces CD8+CD44+ memory T cells in BD mice 20 F. Poly I:C up-regulate CD122(IL-15/2Rβ) and CD8+CD122+ T cells in BD mice 24 G. Poly I:C down-regulates IL-23 receptor and IL-17A in BD mice 28 Ⅳ. DISCUSSION 30 Ⅴ. CONCLUSION 34 REFERENCES 36 국문요약 45","Master"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/7539","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000012284","000000012284"],"dc:language":["en"],"dc:subject":["Behcet's disease","IL-15Rα","Poly I:C","memory CD8+ T cells","Herpes Simplex virus","mouse model"],"dc:title":["Anti-inflammatory Role of IL-15Rα Up-regulated by Poly I:C in Herpes Simplex Virus-induced Behcet's Disease-like Mouse Model","단순포진 바이러스로 유도한 베체트병 마우스모델에서 Poly I:C 에 의해 유도된 IL-15Rα의 항 염증 효과에 관한 연구"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:39Z"}