Association between Chronic Hepatitis B Virus Infection and Interleukin-10, Tumor Necrosis Factor-α Gene Promoter Polymorphisms

The reasons for the viral persistence of hepatitis B virus (HBV) infection are unknown, but are probably related to host immune factors. Cytokines play significant roles in inflammatory and immune defense. This study was undertaken to investigate the association between HBV infection and polymorphisms of tumor necrosis factor-a (TNF-α) and interleukin-10 (IL-10) gene promoter. We studied 412 Korean patients with HBV infection (72 inactive carriers, 261 chronic hepatitis, 79 liver cirrhosis) and 204 healthy individuals who recovered from HBV infection. We assessed polymorphisms in IL-10 gene promoter (-1082, -819, -592), and TNF-α gene promoter (-308, -238) by single base primer extension assay. The frequency of C/C genotype at position -592 of IL-10 gene promoter was higher in the HBV clearance group than that in the persistence group in univariate analysis (12.7% vs. 7.5%, p=0.036). IL-10 gene promoter -592 C/C genotype was related to clearance of HBV infection in logistic regression analysis after adjusting age and sex (p=0.003). Genotype frequencies of TNF-α gene promoter at positions -308 and -238 were not different between the clearance and the persistence group in univariate analysis, but in multivariate analysis after adjusting age and sex, -308G / -238G homozygotes were associated with HBV persistence (p=0.005). Genotype distributions of both gene promoters in inactive carriers were similar to those in patients with chronic progressive liver disease. In conclusion, both the carriers of -592 A allele in the IL-10 promoter and -308G / -238 G haplotype homozygotes in the TNF-α promoter region have higher risk of persistent HBV infection.

Author dc:creator

• 정, 재연

Contributors dc:contributor

• 조, 성원
• 대학원 의학과
• 104614

• chronic hepatitis B
• interleukin-10 (IL-10)
• tumor necrosis factor-α (TNF-α)
• single nucleotide polymorphism (SNP)
• 만성 B 형 간염

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000411
000000000411

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/2284