Identification of Undifferentiated Human Embryonic Stem Cell-Specific Genes

Embryonic stem cells are derived from mammalian blastocysts and self-renewal and maintain pluripotency, an ability to differentiate into all types of somatic and germ cells. Recent reports showed that several genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc(c-Myc) maintained a pluripotent undifferentiated state. As we know, both stem cells and cancer cells have self-renewal ability. To investigate for the mechanism that maintain the self-renewal and proliferation ability, we identified 11 genes that are specifically and commonly expressed in human ES cells and various cancer cells by digital differential display of expressed sequence tag databases. Then, we confirmed differential expression of these genes by RT-PCR in human embryonic stem cell, neural stem cells and normal tissues, and identified that 3 genes, KIAA1922, SELV, and OR56B1. These genes are strongly expressed human embryonic stem cells in their undifferentiated state and represent the core key mammalian stemness factors. Understanding the molecular mechanism by which self-renewal is maintained in human embryonic stem cells is important for the development of improved methods to derive, culture and differentiate these into cells of potential therapeutics.

Author dc:creator

• 김, 동철

Contributors dc:contributor

• 이, 명애
• 대학원 의생명과학과
• 200724508

• 배아줄기세포
• 다능성

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010356
000000010356

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/2258