{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/1622"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/1622","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"The Effect of Tim-3-Ig on Tumor Growth","abstract":"Tim-3, a member of the novel TIM (T cell immunoglobulin- and mucin-containing-domain) family of molecules, is the first molecule identified to be specifically expressed on CD4+ Th1 and not on Th2 cells. Tim-3 negatively regulates Th1 immunity and induces tolerance, but it's function has not been clarified in tumor immunity. To explore the effect of Tim pathway blockade on tumor growth using Tim-3-Ig fusion protein, two approaches were adopted. First, it was investigated whether Tim-3-Ig expression in lung carcinoma cells (3LL) affects tumor growth in mice. Second, it was investigated whether administration of purified Tim-3-Ig into mice influences tumor growth. Tumor growth of 3LL cells expressing Tim-3-Ig was suppressed as compared with control mouse groups. Further tumor growth in mice injected with purified Tim-3-Ig was inhibited as compared with control mouse groups injected with human Ig and PBS, respectively. Additionally, Mammal I revealed the importance of Tim-3 glycosylation for its binding activity to Tim-3 ligand using Tim-3-Ig expressed by mammalian cells and by Sf 9 cells. My results showed suppressive effect of Tim-3-Ig on tumor growth, implying significance of Tim-3-Tim-3 ligand pathway in tumor immunology.","abstract_html":"Tim-3, a member of the novel TIM (T cell immunoglobulin- and mucin-containing-domain) family of molecules, is the first molecule identified to be specifically expressed on CD4+ Th1 and not on Th2 cells. Tim-3 negatively regulates Th1 immunity and induces tolerance, but it's function has not been clarified in tumor immunity. To explore the effect of Tim pathway blockade on tumor growth using Tim-3-Ig fusion protein, two approaches were adopted. First, it was investigated whether Tim-3-Ig expression in lung carcinoma cells (3LL) affects tumor growth in mice. Second, it was investigated whether administration of purified Tim-3-Ig into mice influences tumor growth. Tumor growth of 3LL cells expressing Tim-3-Ig was suppressed as compared with control mouse groups. Further tumor growth in mice injected with purified Tim-3-Ig was inhibited as compared with control mouse groups injected with human Ig and PBS, respectively. Additionally, Mammal I revealed the importance of Tim-3 glycosylation for its binding activity to Tim-3 ligand using Tim-3-Ig expressed by mammalian cells and by Sf 9 cells. My results showed suppressive effect of Tim-3-Ig on tumor growth, implying significance of Tim-3-Tim-3 ligand pathway in tumor immunology.","abstract_has_math":false,"creators":["허, 유미"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["박, 선","대학원 의학과","200424344"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-03-10","date_published":"2011-03-10","updated_at":"2026-07-24T00:51:24Z","subjects":["T cell immunoglobulin- and mucin domain-3 (Tim-3)","종양 면역","당화","3LL","역전사"],"languages":["ko"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001872","000000001872"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001872","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001872","code":true},{"text":"000000001872","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/1622","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["박, 선","대학원 의학과","200424344","허, 유미"]},{"key":"dc:creator","label":"Author","values":["허, 유미"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-03-10","2007"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["T cell immunoglobulin- and mucin domain-3 (Tim-3)","종양 면역","당화","3LL","역전사"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ko"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/1622","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001872","000000001872"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Tim-3, a member of the novel TIM (T cell immunoglobulin- and mucin-containing-domain) family of molecules, is the first molecule identified to be specifically expressed on CD4+ Th1 and not on Th2 cells. Tim-3 negatively regulates Th1 immunity and induces tolerance, but it's function has not been clarified in tumor immunity. To explore the effect of Tim pathway blockade on tumor growth using Tim-3-Ig fusion protein, two approaches were adopted. First, it was investigated whether Tim-3-Ig expression in lung carcinoma cells (3LL) affects tumor growth in mice. Second, it was investigated whether administration of purified Tim-3-Ig into mice influences tumor growth. Tumor growth of 3LL cells expressing Tim-3-Ig was suppressed as compared with control mouse groups. Further tumor growth in mice injected with purified Tim-3-Ig was inhibited as compared with control mouse groups injected with human Ig and PBS, respectively. Additionally, Mammal I revealed the importance of Tim-3 glycosylation for its binding activity to Tim-3 ligand using Tim-3-Ig expressed by mammalian cells and by Sf 9 cells. My results showed suppressive effect of Tim-3-Ig on tumor growth, implying significance of Tim-3-Tim-3 ligand pathway in tumor immunology.","Tim-3는 T 세포 immunoglobulin- and mucin-domain-containing molecules(TIMs)의 한 family로서 보조 T 세포 1형에서 특이적으로 발현하는 분자로 발견되었다. 이 분자는 그 ligand와 결합하여 Th1 세포의 면역반응을 억제하고 면역관용 유도에 관여한다고 알려져 있으나 종양면역에서 Tim-3의 역할에 대해서는 많이 연구된 바 없다. 본 연구에서는 Tim-3와 Tim-3 ligand 결합을 방해할 수 있는 Tim-3-Ig(Tim-3와 면역글로불린의 융합 단백질)이 종양 성장에 미치는 영향을 알아보고자 하였다. 본 연구에서 두 가지 연구 방법을 취하였다. 첫째, Tim-3-Ig을 발현하도록 만든 폐 종양 세포주 3LL의 종양 성장을 대조군 세포의 종양 성장과 비교하였다. 둘째, Tim-3-Ig을 생산 정제하여 3LL 종양 모델에 투여한 후 3LL의 종양 성장을 관찰하였다. 그 결과 3LL 세포의 Tim-3-Ig 발현 혹은 정제된 Tim-3-Ig의 투여에 의해 마우스 내 3LL 종양 성장이 억제되었다. 부수적으로 포유동물 세포와 곤충세포를 이용한 Tim-3-Ig의 생산법을 비교하여 Tim-3-Ig의 glycosylation이 Tim-3―Tim-3L 결합에 중요함을 관찰하였다. 본 연구 결과는 Tim-3-Ig이 종양성장에 억제작용을 할 수 있음을 밝힘으로써 종양면역에서 Tim-3―Tim-3L 경로의 중요성을 시사한다.","\"Ⅰ. 서론 = 1 A. Tim-3 = 1 B. Baculovirus를 이용한 단백질 발현 시스템 = 4 C. 종양과 Tim-3 = 4 D. 연구 목적 = 5 Ⅱ. 재료 및 방법 = 6 A. Tim-3-Ig 발현 벡터의 제조 = 6 B. Tim-3-Ig 발현 벡터의 이입 = 9 C. Western Blot Analysis = 10 D. 친화성 크로마토그래피와 고분자 액체 크로마토그래피 = 10 E. 마우스 CD4+ T 세포의 분리 = 11 F. 유세포 분석 = 12 G. 역전사 중합 효소 연쇄 반응 = 12 H. 재조합 baculovirus 생산과 곤충 세포 배양 = 13 I. 마우스에서 종양 성장 측정 = 14 Ⅲ. 결과 = 15 A. Tim-3 아미노산 서열 분석 = 15 B. Tim-3-Ig 발현 벡터 생산 = 16 C. 포유동물 세포에서 Tim-3-Ig의 생산·정제 및 리간드 결합능 분석 = 18 D. Tim-3-Ig을 발현하는 3LL 세포주 확립 = 21 E. 3LL 세포의 Tim-3-Ig 발현이 종양 성장에 미치는 영향 = 23 F. 곤충 세포에서 Tim-3-Ig의 생산과 정제 및 이의 리간드 결합능 분석 = 26 G. Tim-3-Ig의 투여가 종양의 성장에 미치는 영향 = 28 Ⅳ. 고찰 = 30 Ⅴ. 결론 = 33 참고문헌 = 34 ABSTRACT = 43\"","Master"]},{"key":"dc:title","label":"Title","values":["The Effect of Tim-3-Ig on Tumor Growth","Tim-3-Ig이 종양 성장에 미치는 영향"]}]}],"canonical_facts":{"dc:contributor":["박, 선","대학원 의학과","200424344","허, 유미"],"dc:creator":["허, 유미"],"dc:date":["2011-03-10","2007"],"dc:description":["Tim-3, a member of the novel TIM (T cell immunoglobulin- and mucin-containing-domain) family of molecules, is the first molecule identified to be specifically expressed on CD4+ Th1 and not on Th2 cells. Tim-3 negatively regulates Th1 immunity and induces tolerance, but it's function has not been clarified in tumor immunity. To explore the effect of Tim pathway blockade on tumor growth using Tim-3-Ig fusion protein, two approaches were adopted. First, it was investigated whether Tim-3-Ig expression in lung carcinoma cells (3LL) affects tumor growth in mice. Second, it was investigated whether administration of purified Tim-3-Ig into mice influences tumor growth. Tumor growth of 3LL cells expressing Tim-3-Ig was suppressed as compared with control mouse groups. Further tumor growth in mice injected with purified Tim-3-Ig was inhibited as compared with control mouse groups injected with human Ig and PBS, respectively. Additionally, Mammal I revealed the importance of Tim-3 glycosylation for its binding activity to Tim-3 ligand using Tim-3-Ig expressed by mammalian cells and by Sf 9 cells. My results showed suppressive effect of Tim-3-Ig on tumor growth, implying significance of Tim-3-Tim-3 ligand pathway in tumor immunology.","Tim-3는 T 세포 immunoglobulin- and mucin-domain-containing molecules(TIMs)의 한 family로서 보조 T 세포 1형에서 특이적으로 발현하는 분자로 발견되었다. 이 분자는 그 ligand와 결합하여 Th1 세포의 면역반응을 억제하고 면역관용 유도에 관여한다고 알려져 있으나 종양면역에서 Tim-3의 역할에 대해서는 많이 연구된 바 없다. 본 연구에서는 Tim-3와 Tim-3 ligand 결합을 방해할 수 있는 Tim-3-Ig(Tim-3와 면역글로불린의 융합 단백질)이 종양 성장에 미치는 영향을 알아보고자 하였다. 본 연구에서 두 가지 연구 방법을 취하였다. 첫째, Tim-3-Ig을 발현하도록 만든 폐 종양 세포주 3LL의 종양 성장을 대조군 세포의 종양 성장과 비교하였다. 둘째, Tim-3-Ig을 생산 정제하여 3LL 종양 모델에 투여한 후 3LL의 종양 성장을 관찰하였다. 그 결과 3LL 세포의 Tim-3-Ig 발현 혹은 정제된 Tim-3-Ig의 투여에 의해 마우스 내 3LL 종양 성장이 억제되었다. 부수적으로 포유동물 세포와 곤충세포를 이용한 Tim-3-Ig의 생산법을 비교하여 Tim-3-Ig의 glycosylation이 Tim-3―Tim-3L 결합에 중요함을 관찰하였다. 본 연구 결과는 Tim-3-Ig이 종양성장에 억제작용을 할 수 있음을 밝힘으로써 종양면역에서 Tim-3―Tim-3L 경로의 중요성을 시사한다.","\"Ⅰ. 서론 = 1 A. Tim-3 = 1 B. Baculovirus를 이용한 단백질 발현 시스템 = 4 C. 종양과 Tim-3 = 4 D. 연구 목적 = 5 Ⅱ. 재료 및 방법 = 6 A. Tim-3-Ig 발현 벡터의 제조 = 6 B. Tim-3-Ig 발현 벡터의 이입 = 9 C. Western Blot Analysis = 10 D. 친화성 크로마토그래피와 고분자 액체 크로마토그래피 = 10 E. 마우스 CD4+ T 세포의 분리 = 11 F. 유세포 분석 = 12 G. 역전사 중합 효소 연쇄 반응 = 12 H. 재조합 baculovirus 생산과 곤충 세포 배양 = 13 I. 마우스에서 종양 성장 측정 = 14 Ⅲ. 결과 = 15 A. Tim-3 아미노산 서열 분석 = 15 B. Tim-3-Ig 발현 벡터 생산 = 16 C. 포유동물 세포에서 Tim-3-Ig의 생산·정제 및 리간드 결합능 분석 = 18 D. Tim-3-Ig을 발현하는 3LL 세포주 확립 = 21 E. 3LL 세포의 Tim-3-Ig 발현이 종양 성장에 미치는 영향 = 23 F. 곤충 세포에서 Tim-3-Ig의 생산과 정제 및 이의 리간드 결합능 분석 = 26 G. Tim-3-Ig의 투여가 종양의 성장에 미치는 영향 = 28 Ⅳ. 고찰 = 30 Ⅴ. 결론 = 33 참고문헌 = 34 ABSTRACT = 43\"","Master"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/1622","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001872","000000001872"],"dc:language":["ko"],"dc:subject":["T cell immunoglobulin- and mucin domain-3 (Tim-3)","종양 면역","당화","3LL","역전사"],"dc:title":["The Effect of Tim-3-Ig on Tumor Growth","Tim-3-Ig이 종양 성장에 미치는 영향"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:24Z"}