{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/1501"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/1501","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"Anti-oxidant Action and Anti-aging Activity of Phenylpropanoid Compounds","abstract":"Purpose: To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and anti-inflammatory activity and effect of caffeic acid on wound healing in skin-incised mice. Materials & methods: The cinnamic acid derivaties, C_(6)-C₃ compounds, of Penylpropanoids are used. To investigegate the effects of anti-oxidant activity in cell free system, we measured DPPH radical scavenging assay, xanthine oxidase activity, NBT/xanthine oxidase superoxide scavenging assay. In cell system superoxide production using DHE, H₂O₂ production using DCF-DA and hydroperoxide production using DHR were checked. In order to find out the effects of anti-inflammatory activity, histamine assay, arachidonic acid release and prostaglandin E₂ assay in RBL 2H3 mast cells were measured and NIH 3T3 cell was used for collagen synthesis assay. Balb-c mouse were used to measure collagen assay, MPO assay, PLA₂ activity and lipid peroxidation in vivo system. Result: In DPPH radical scavenging activity, caffeic acid analogues had anti-oxidant activity in a dose-dependent manner, whereas cinnamic acid and coumaric acid did not. Both caffeic acid and chlorogenic acid significantly showed anti-oxidant activity in NBT/XO superoxide anion scavenging assay but others did not. These results suggest that anti-oxidant activity of phenylpropanoid may be related to the hydroxyl residues in aromatic ring. Silica dose-dependently increased the intracellular ROS generation in RBL 2H3 cells. Although phenylpropanoids did not inhibit intracellular superoxide anion generation, both caffeic acid and chlorogenic acid significantly inhibited silica-induced intracellular H₂O₂ and generation hydroxyl radical in RBL 2H3 cells. On the other hand, melittin, an endogenous phospholipase A₂ activator, dose-dependently increased both histamine release and arachidonic acid release. Melittin-induced histamine release were dose-dependently inhibited by caffeic acid and chlorogenic acid, whereas melittin-induced arachidonic acid release was not affected by any of phenylpropanoids. However, melittin-induced prostaglandin E₂ production was significantly inhibited by caffeic, ferulic, sinapinic and chlorogenic acid. These data suggest that inhibitory action of phenylpropanoid on prostaglandin E₂ production may be due to the inhibition of cyclooxygenase rather than phospholipase A₂. In NIH 3T3 fibroblast cells, caffeic acid significantly increased collagen-like polymer synthesis, which suggest that caffeic acid appears to be effective on wound healing. The significant effect of caffeic acid on anti-inflammatory activity and wound healing, such as myeloperoxidase activity, lipid peroxidation, phospholipase A₂ activity and collagen-like polymer synthesis, showed in incised wound mice. Conclusion: From the above results, it is referred that both caffeic acid and chlorogenic acid appear to have potent anti-oxidant and anti-inflammatory activity. In particular, considering the inhibitory activity of caffeic acid among phenylpropanoids in in vitro/cell assay system and in vivo system, it is suggested that the hydroxyl residues of aromatic ring plays an important role in anti-oxidant, anti-inflammatory activity and wound healing effect.","abstract_html":"Purpose: To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and anti-inflammatory activity and effect of caffeic acid on wound healing in skin-incised mice. Materials & methods: The cinnamic acid derivaties, C_(6)-C₃ compounds, of Penylpropanoids are used. To investigegate the effects of anti-oxidant activity in cell free system, we measured DPPH radical scavenging assay, xanthine oxidase activity, NBT/xanthine oxidase superoxide scavenging assay. In cell system superoxide production using DHE, H₂O₂ production using DCF-DA and hydroperoxide production using DHR were checked. In order to find out the effects of anti-inflammatory activity, histamine assay, arachidonic acid release and prostaglandin E₂ assay in RBL 2H3 mast cells were measured and NIH 3T3 cell was used for collagen synthesis assay. Balb-c mouse were used to measure collagen assay, MPO assay, PLA₂ activity and lipid peroxidation in vivo system. Result: In DPPH radical scavenging activity, caffeic acid analogues had anti-oxidant activity in a dose-dependent manner, whereas cinnamic acid and coumaric acid did not. Both caffeic acid and chlorogenic acid significantly showed anti-oxidant activity in NBT/XO superoxide anion scavenging assay but others did not. These results suggest that anti-oxidant activity of phenylpropanoid may be related to the hydroxyl residues in aromatic ring. Silica dose-dependently increased the intracellular ROS generation in RBL 2H3 cells. Although phenylpropanoids did not inhibit intracellular superoxide anion generation, both caffeic acid and chlorogenic acid significantly inhibited silica-induced intracellular H₂O₂ and generation hydroxyl radical in RBL 2H3 cells. On the other hand, melittin, an endogenous phospholipase A₂ activator, dose-dependently increased both histamine release and arachidonic acid release. Melittin-induced histamine release were dose-dependently inhibited by caffeic acid and chlorogenic acid, whereas melittin-induced arachidonic acid release was not affected by any of phenylpropanoids. However, melittin-induced prostaglandin E₂ production was significantly inhibited by caffeic, ferulic, sinapinic and chlorogenic acid. These data suggest that inhibitory action of phenylpropanoid on prostaglandin E₂ production may be due to the inhibition of cyclooxygenase rather than phospholipase A₂. In NIH 3T3 fibroblast cells, caffeic acid significantly increased collagen-like polymer synthesis, which suggest that caffeic acid appears to be effective on wound healing. The significant effect of caffeic acid on anti-inflammatory activity and wound healing, such as myeloperoxidase activity, lipid peroxidation, phospholipase A₂ activity and collagen-like polymer synthesis, showed in incised wound mice. Conclusion: From the above results, it is referred that both caffeic acid and chlorogenic acid appear to have potent anti-oxidant and anti-inflammatory activity. In particular, considering the inhibitory activity of caffeic acid among phenylpropanoids in in vitro/cell assay system and in vivo system, it is suggested that the hydroxyl residues of aromatic ring plays an important role in anti-oxidant, anti-inflammatory activity and wound healing effect.","abstract_has_math":false,"creators":["임, 혜원"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["정, 윤석","대학원 의학과","200224485"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-03-02T01:15:31Z","date_published":"2011-03-02T01:15:31Z","updated_at":"2026-07-24T00:51:22Z","subjects":["phenylpropanoids","inflammation","ROS","histamine","arachidonic acid","collagen"],"languages":["ko"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000681","000000000681"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000681","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000681","code":true},{"text":"000000000681","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/1501","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["정, 윤석","대학원 의학과","200224485","임, 혜원"]},{"key":"dc:creator","label":"Author","values":["임, 혜원"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-03-02T01:15:31Z","2005"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["phenylpropanoids","inflammation","ROS","histamine","arachidonic acid","collagen"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ko"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/1501","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000681","000000000681"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Purpose: To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and anti-inflammatory activity and effect of caffeic acid on wound healing in skin-incised mice. Materials & methods: The cinnamic acid derivaties, C_(6)-C₃ compounds, of Penylpropanoids are used. To investigegate the effects of anti-oxidant activity in cell free system, we measured DPPH radical scavenging assay, xanthine oxidase activity, NBT/xanthine oxidase superoxide scavenging assay. In cell system superoxide production using DHE, H₂O₂ production using DCF-DA and hydroperoxide production using DHR were checked. In order to find out the effects of anti-inflammatory activity, histamine assay, arachidonic acid release and prostaglandin E₂ assay in RBL 2H3 mast cells were measured and NIH 3T3 cell was used for collagen synthesis assay. Balb-c mouse were used to measure collagen assay, MPO assay, PLA₂ activity and lipid peroxidation in vivo system. Result: In DPPH radical scavenging activity, caffeic acid analogues had anti-oxidant activity in a dose-dependent manner, whereas cinnamic acid and coumaric acid did not. Both caffeic acid and chlorogenic acid significantly showed anti-oxidant activity in NBT/XO superoxide anion scavenging assay but others did not. These results suggest that anti-oxidant activity of phenylpropanoid may be related to the hydroxyl residues in aromatic ring. Silica dose-dependently increased the intracellular ROS generation in RBL 2H3 cells. Although phenylpropanoids did not inhibit intracellular superoxide anion generation, both caffeic acid and chlorogenic acid significantly inhibited silica-induced intracellular H₂O₂ and generation hydroxyl radical in RBL 2H3 cells. On the other hand, melittin, an endogenous phospholipase A₂ activator, dose-dependently increased both histamine release and arachidonic acid release. Melittin-induced histamine release were dose-dependently inhibited by caffeic acid and chlorogenic acid, whereas melittin-induced arachidonic acid release was not affected by any of phenylpropanoids. However, melittin-induced prostaglandin E₂ production was significantly inhibited by caffeic, ferulic, sinapinic and chlorogenic acid. These data suggest that inhibitory action of phenylpropanoid on prostaglandin E₂ production may be due to the inhibition of cyclooxygenase rather than phospholipase A₂. In NIH 3T3 fibroblast cells, caffeic acid significantly increased collagen-like polymer synthesis, which suggest that caffeic acid appears to be effective on wound healing. The significant effect of caffeic acid on anti-inflammatory activity and wound healing, such as myeloperoxidase activity, lipid peroxidation, phospholipase A₂ activity and collagen-like polymer synthesis, showed in incised wound mice. Conclusion: From the above results, it is referred that both caffeic acid and chlorogenic acid appear to have potent anti-oxidant and anti-inflammatory activity. In particular, considering the inhibitory activity of caffeic acid among phenylpropanoids in in vitro/cell assay system and in vivo system, it is suggested that the hydroxyl residues of aromatic ring plays an important role in anti-oxidant, anti-inflammatory activity and wound healing effect.","Doctor"]},{"key":"dc:format","label":"Dc Format","values":["text/plain"]},{"key":"dc:title","label":"Title","values":["Anti-oxidant Action and Anti-aging Activity of Phenylpropanoid Compounds","Phenylpropanoid 화합물의 항산화 및 항노화 작용"]}]}],"canonical_facts":{"dc:contributor":["정, 윤석","대학원 의학과","200224485","임, 혜원"],"dc:creator":["임, 혜원"],"dc:date":["2011-03-02T01:15:31Z","2005"],"dc:description":["Purpose: To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and anti-inflammatory activity and effect of caffeic acid on wound healing in skin-incised mice. Materials & methods: The cinnamic acid derivaties, C_(6)-C₃ compounds, of Penylpropanoids are used. To investigegate the effects of anti-oxidant activity in cell free system, we measured DPPH radical scavenging assay, xanthine oxidase activity, NBT/xanthine oxidase superoxide scavenging assay. In cell system superoxide production using DHE, H₂O₂ production using DCF-DA and hydroperoxide production using DHR were checked. In order to find out the effects of anti-inflammatory activity, histamine assay, arachidonic acid release and prostaglandin E₂ assay in RBL 2H3 mast cells were measured and NIH 3T3 cell was used for collagen synthesis assay. Balb-c mouse were used to measure collagen assay, MPO assay, PLA₂ activity and lipid peroxidation in vivo system. Result: In DPPH radical scavenging activity, caffeic acid analogues had anti-oxidant activity in a dose-dependent manner, whereas cinnamic acid and coumaric acid did not. Both caffeic acid and chlorogenic acid significantly showed anti-oxidant activity in NBT/XO superoxide anion scavenging assay but others did not. These results suggest that anti-oxidant activity of phenylpropanoid may be related to the hydroxyl residues in aromatic ring. Silica dose-dependently increased the intracellular ROS generation in RBL 2H3 cells. Although phenylpropanoids did not inhibit intracellular superoxide anion generation, both caffeic acid and chlorogenic acid significantly inhibited silica-induced intracellular H₂O₂ and generation hydroxyl radical in RBL 2H3 cells. On the other hand, melittin, an endogenous phospholipase A₂ activator, dose-dependently increased both histamine release and arachidonic acid release. Melittin-induced histamine release were dose-dependently inhibited by caffeic acid and chlorogenic acid, whereas melittin-induced arachidonic acid release was not affected by any of phenylpropanoids. However, melittin-induced prostaglandin E₂ production was significantly inhibited by caffeic, ferulic, sinapinic and chlorogenic acid. These data suggest that inhibitory action of phenylpropanoid on prostaglandin E₂ production may be due to the inhibition of cyclooxygenase rather than phospholipase A₂. In NIH 3T3 fibroblast cells, caffeic acid significantly increased collagen-like polymer synthesis, which suggest that caffeic acid appears to be effective on wound healing. The significant effect of caffeic acid on anti-inflammatory activity and wound healing, such as myeloperoxidase activity, lipid peroxidation, phospholipase A₂ activity and collagen-like polymer synthesis, showed in incised wound mice. Conclusion: From the above results, it is referred that both caffeic acid and chlorogenic acid appear to have potent anti-oxidant and anti-inflammatory activity. In particular, considering the inhibitory activity of caffeic acid among phenylpropanoids in in vitro/cell assay system and in vivo system, it is suggested that the hydroxyl residues of aromatic ring plays an important role in anti-oxidant, anti-inflammatory activity and wound healing effect.","Doctor"],"dc:format":["text/plain"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/1501","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000681","000000000681"],"dc:language":["ko"],"dc:subject":["phenylpropanoids","inflammation","ROS","histamine","arachidonic acid","collagen"],"dc:title":["Anti-oxidant Action and Anti-aging Activity of Phenylpropanoid Compounds","Phenylpropanoid 화합물의 항산화 및 항노화 작용"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:22Z"}