{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/1482"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/1482","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"The regulation of expression level in NeuroD/BETA2 by PKA","abstract":"Post-translational regulation of bHLH transcription factors could be effected by phosphorylation, then NeuroD/BETA2 could be effected by phosphorylation too. NeuroD/BETA2 was phosphorylated at Ser336 by CaMKII, then formation of dendirte could be effected by phosphorylation of NeuroD/BETA2. NeuroD/BETA2 might be regulated by phosphorylation at post-translation level. For searching effect of phosphorylation of NeuroD/BETA2, We found kinase and phosphoryation site of NeuroD/BETA2. Treatment of 293T cells with forskolin overexpressed NeuroD/BETA2, expression of NeuroD/BETA2 was increased. And NeuroD/BETA2 could be phosphorylate at N-terminous region by PKA. We predicted that phosphorylation site of NeuroD/BETA2 was at Thr91, using databases: Netphos, PROSITE and ScanProsite.","abstract_html":"Post-translational regulation of bHLH transcription factors could be effected by phosphorylation, then NeuroD/BETA2 could be effected by phosphorylation too. NeuroD/BETA2 was phosphorylated at Ser336 by CaMKII, then formation of dendirte could be effected by phosphorylation of NeuroD/BETA2. NeuroD/BETA2 might be regulated by phosphorylation at post-translation level. For searching effect of phosphorylation of NeuroD/BETA2, We found kinase and phosphoryation site of NeuroD/BETA2. Treatment of 293T cells with forskolin overexpressed NeuroD/BETA2, expression of NeuroD/BETA2 was increased. And NeuroD/BETA2 could be phosphorylate at N-terminous region by PKA. We predicted that phosphorylation site of NeuroD/BETA2 was at Thr91, using databases: Netphos, PROSITE and ScanProsite.","abstract_has_math":false,"creators":["신, 영국"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["이, 영돈","대학원 의학과","200324271"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-02-18T06:34:22Z","date_published":"2011-02-18T06:34:22Z","updated_at":"2026-07-24T00:51:22Z","subjects":["bHLH","NeuroD/BETA2","인산화","인산화 효소","PKA","phosphorylation","kinase","protein stability"],"languages":["ko"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000183","000000000183"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000183","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000183","code":true},{"text":"000000000183","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/1482","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["이, 영돈","대학원 의학과","200324271","신, 영국"]},{"key":"dc:creator","label":"Author","values":["신, 영국"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-02-18T06:34:22Z","2005"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["bHLH","NeuroD/BETA2","인산화","인산화 효소","PKA","phosphorylation","kinase","protein stability"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ko"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/1482","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000183","000000000183"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Post-translational regulation of bHLH transcription factors could be effected by phosphorylation, then NeuroD/BETA2 could be effected by phosphorylation too. NeuroD/BETA2 was phosphorylated at Ser336 by CaMKII, then formation of dendirte could be effected by phosphorylation of NeuroD/BETA2. NeuroD/BETA2 might be regulated by phosphorylation at post-translation level. For searching effect of phosphorylation of NeuroD/BETA2, We found kinase and phosphoryation site of NeuroD/BETA2. Treatment of 293T cells with forskolin overexpressed NeuroD/BETA2, expression of NeuroD/BETA2 was increased. And NeuroD/BETA2 could be phosphorylate at N-terminous region by PKA. We predicted that phosphorylation site of NeuroD/BETA2 was at Thr91, using databases: Netphos, PROSITE and ScanProsite.","bHLH 전사인자들에 대한 post-translational level에서의 조절은 인산화에 의해서 영향을 받을 수 있다. CaMKII에 의해 Ser336에서 인산화된 NeuroD/BETA2는 신경세포의 수상돌기 형성에 영향을 미친다. NeuroD/BETA2가 huntingtin(htt) 단백질과 결합하게 되면 MLK2에 의해 인산화되어 기능이 증가된다는 보고도 있다. 따라서 bHLH 전사인자 중 class B family에 속하는 NeuroD/BETA2의 조절 역시 인산화에 의해 영향을 받을 것이다. 이러한 NeuroD/BETA2 인산화의 영향을 연구하기 위하여 본 연구에서는 NeuroD/BETA2에 관여하는 인산화 효소와 인산화 위치를 찾고자 하였다. 293T 세포에서 NeuroD/BETA2를 발현시킨 후 PKA를 활성화 시켰을때, NeuroD/BETA2의 발현양은 증가하였으며 PKA와 NeuroD/BETA2의 in vitro kinase assay 결과 PKA가 NeuroD/BETA2를 N-말단 쪽에서 인산화 시키는 것이 확인 되었다. 생물정보 데이터베이스들을 이용하여 NeuroD/BETA2의 인산화 위치를 예측하였을 때, N-말단으로부터 아미노산 서열 91번째의 threonine에서 PKA에 의한 인산화가 일어날 것으로 예상된다.","\"차례 국문요약 = ⅰ 차례 = ⅱ 그림차례 = ⅲ 표차례 = ⅳ Ⅰ. 서론 = 1 Ⅱ. 재료 및 방법 = 5 A. 실험재료 = 5 B. 실험방법 = 6 1. 벡터 제작 = 6 2. 세포 배양 및 형질전환 = 8 3. 단백질 획득 = 9 4. Western 분석 = 10 5. EMSA (Electrophoretic Mobility Shift Assay) = 10 6. GST-NeuroD 융합단백질을 이용한 in vitro kinase assay = 11 7. NeuroD/BETA2의 인산화 위치 예측 = 12 Ⅲ. 결과 = 13 A. NeuroD/BETA2의 발현 확인 = 13 B. PKA 활성화에 의한 NeuroD/BETA2의 발현 조절 = 13 C. NeuroD/BETA2의 인산화 확인 = 25 1. PKA에 의한 NeuroD/BETA2의 인산화 = 25 2. NeuroD/BETA2의 인산화 위치 예측 = 26 Ⅳ. 고찰 = 28 Ⅴ. 결론 = 32 참고문헌 = 33 ABSTRACT = 38\"","Master"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The regulation of expression level in NeuroD/BETA2 by PKA","PKA 인산화에 의한 NeuroD/BETA2의 발현 조절"]}]}],"canonical_facts":{"dc:contributor":["이, 영돈","대학원 의학과","200324271","신, 영국"],"dc:creator":["신, 영국"],"dc:date":["2011-02-18T06:34:22Z","2005"],"dc:description":["Post-translational regulation of bHLH transcription factors could be effected by phosphorylation, then NeuroD/BETA2 could be effected by phosphorylation too. NeuroD/BETA2 was phosphorylated at Ser336 by CaMKII, then formation of dendirte could be effected by phosphorylation of NeuroD/BETA2. NeuroD/BETA2 might be regulated by phosphorylation at post-translation level. For searching effect of phosphorylation of NeuroD/BETA2, We found kinase and phosphoryation site of NeuroD/BETA2. Treatment of 293T cells with forskolin overexpressed NeuroD/BETA2, expression of NeuroD/BETA2 was increased. And NeuroD/BETA2 could be phosphorylate at N-terminous region by PKA. We predicted that phosphorylation site of NeuroD/BETA2 was at Thr91, using databases: Netphos, PROSITE and ScanProsite.","bHLH 전사인자들에 대한 post-translational level에서의 조절은 인산화에 의해서 영향을 받을 수 있다. CaMKII에 의해 Ser336에서 인산화된 NeuroD/BETA2는 신경세포의 수상돌기 형성에 영향을 미친다. NeuroD/BETA2가 huntingtin(htt) 단백질과 결합하게 되면 MLK2에 의해 인산화되어 기능이 증가된다는 보고도 있다. 따라서 bHLH 전사인자 중 class B family에 속하는 NeuroD/BETA2의 조절 역시 인산화에 의해 영향을 받을 것이다. 이러한 NeuroD/BETA2 인산화의 영향을 연구하기 위하여 본 연구에서는 NeuroD/BETA2에 관여하는 인산화 효소와 인산화 위치를 찾고자 하였다. 293T 세포에서 NeuroD/BETA2를 발현시킨 후 PKA를 활성화 시켰을때, NeuroD/BETA2의 발현양은 증가하였으며 PKA와 NeuroD/BETA2의 in vitro kinase assay 결과 PKA가 NeuroD/BETA2를 N-말단 쪽에서 인산화 시키는 것이 확인 되었다. 생물정보 데이터베이스들을 이용하여 NeuroD/BETA2의 인산화 위치를 예측하였을 때, N-말단으로부터 아미노산 서열 91번째의 threonine에서 PKA에 의한 인산화가 일어날 것으로 예상된다.","\"차례 국문요약 = ⅰ 차례 = ⅱ 그림차례 = ⅲ 표차례 = ⅳ Ⅰ. 서론 = 1 Ⅱ. 재료 및 방법 = 5 A. 실험재료 = 5 B. 실험방법 = 6 1. 벡터 제작 = 6 2. 세포 배양 및 형질전환 = 8 3. 단백질 획득 = 9 4. Western 분석 = 10 5. EMSA (Electrophoretic Mobility Shift Assay) = 10 6. GST-NeuroD 융합단백질을 이용한 in vitro kinase assay = 11 7. NeuroD/BETA2의 인산화 위치 예측 = 12 Ⅲ. 결과 = 13 A. NeuroD/BETA2의 발현 확인 = 13 B. PKA 활성화에 의한 NeuroD/BETA2의 발현 조절 = 13 C. NeuroD/BETA2의 인산화 확인 = 25 1. PKA에 의한 NeuroD/BETA2의 인산화 = 25 2. NeuroD/BETA2의 인산화 위치 예측 = 26 Ⅳ. 고찰 = 28 Ⅴ. 결론 = 32 참고문헌 = 33 ABSTRACT = 38\"","Master"],"dc:format":["application/pdf"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/1482","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000183","000000000183"],"dc:language":["ko"],"dc:subject":["bHLH","NeuroD/BETA2","인산화","인산화 효소","PKA","phosphorylation","kinase","protein stability"],"dc:title":["The regulation of expression level in NeuroD/BETA2 by PKA","PKA 인산화에 의한 NeuroD/BETA2의 발현 조절"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:22Z"}