Studies on the Cytoplasmic Inclusions Detected in Parkinson's disease
type: CreativeWork
description: α-Synuclein-positive cytoplasmic inclusions are a pathological hallmark of several neurodegenerative disorders, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). Synphilin-1, interaction partner of α-Synuclein is a major component of inclusion bodies, but it is unknown how synphiln-1 contributes to the cellular and biochemical mechanisms of PD, and its normal functions and biochemical properties are poorly understood. To determine the protein interaction partners of synphilin-1, we performed a yeast two-hybrid screen. We identified a new interacting protein LIM domain only 7 protein, LMO7. This protein localized in the nucleus, cytoplasm and cell surface, particularly adhesion junctions and contains a PDZ and LIM domain, both of which mediate protein-protein interactions. In this study, LMO7 interacts with α-Synuclein interacting protein, synphilin-1 and revealed that the co-expression with synphilin-1 results in the formation of cytoplasmic inclusions in cultured HEK293 and SY5Y cells. Synphilin-1 interacts preferentially with the C-terminal LIM domain of LMO7 and LMO7 interacts with the ankyrin domain of synphilin-1. These findings have important implications for understanding the molecular mechanism by which Lewy-body-associated proteins interact through synphilin-1. We immunostained sections of brains from patients with Parkinson's disease and demonstrated that LMO7, as well as synphilin-1, accumulates in the inclusion bodies. To define the role of LMO7 in the formation of these inclusion bodies, we performed a co-transfection with synphilin-1 and LMO7 using cultured HEK293 cells. This assay showed that LMO7 in the formation of these inclusion bodies promotes the formation of cytoplasmic inclusions. α-Synuclein, synphilin-1 and its interacting partner LMO7 are among constituent proteins in these aggregates. The presence of ubiquitin and proteasome subunits in these inclusions supports a role for this protein …
abstract: α-Synuclein-positive cytoplasmic inclusions are a pathological hallmark of several neurodegenerative disorders, including Parkinson's disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA). Synphilin-1, interaction partner of α-Synuclein is a major component of inclusion bodies, but it is unknown how synphiln-1 contributes to the cellular and biochemical mechanisms of PD, and its normal functions and biochemical properties are poorly understood. To determine the protein interaction partners of synphilin-1, we performed a yeast two-hybrid screen. We identified a new interacting protein LIM domain only 7 protein, LMO7. This protein localized in the nucleus, cytoplasm and cell surface, particularly adhesion junctions and contains a PDZ and LIM domain, both of which mediate protein-protein interactions. In this study, LMO7 interacts with α-Synuclein interacting protein, synphilin-1 and revealed that the co-expression with synphilin-1 results in the formation of cytoplasmic inclusions in cultured HEK293 and SY5Y cells. Synphilin-1 interacts preferentially with the C-terminal LIM domain of LMO7 and LMO7 interacts with the ankyrin domain of synphilin-1. These findings have important implications for understanding the molecular mechanism by which Lewy-body-associated proteins interact through synphilin-1. We immunostained sections of brains from patients with Parkinson's disease and demonstrated that LMO7, as well as synphilin-1, accumulates in the inclusion bodies. To define the role of LMO7 in the formation of these inclusion bodies, we performed a co-transfection with synphilin-1 and LMO7 using cultured HEK293 cells. This assay showed that LMO7 in the formation of these inclusion bodies promotes the formation of cytoplasmic inclusions. α-Synuclein, synphilin-1 and its interacting partner LMO7 are among constituent proteins in these aggregates. The presence of ubiquitin and proteasome subunits in these inclusions supports a role for this protein …
author: 류, 명이
language: en
keywords: Parkinson's disease, Lewy body, Cytoplasmic Inclusions, PDZ, LIM
date published: 2011-01-31T08:21:45Z
date modified: 2026-07-24T00:51:22Z
provider: Ajou University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ajou University