Initial Stages of Hepatitis B Virus Replication
type: CreativeWork
description: PART 1 Intracellular Trafficking of HBV Core Particle in Various Stage of DNA Replication Inside of hepatitis B virus (HBV) core particle, pgRNA is reverse transcribed by HBV DNA polymerase into minus-strand DNA and pregenomic RNA (pgRNA) is degraded concomitantly by RNase H activity of DNA polymerase. Then, plus-strand DNA is synthesized to double-stranded relaxed circular DNA. Through this HBV DNA replication, immature core particle becomes mature core particle. This mature core particle can either be transported back into nucleus to amplify the intracellular viral genome pool, or be enveloped at intracellular membrane to be secreted as a mature virion. In this study, intracellular trafficking of HBV core particles was investigated according to the of HBV DNA replication stages. To obtain the core particles, which represent the various stages of HBV DNA replication, several HBV mutants were constructed. RT YMHA, reverse transcription deficient, mutant can encapsidate pgRNA but DNA replication is completely blocked by mutation of YMDD reverse transcriptase motif to YMHA. Priming deficient TP Y65F mutant core particles, in which tyrosine 65 in TP domain of HBV DNA polymerase was changed to phenylalanine, can synthesize oligomer, the nascent minus-strand DNA. RH D750V mutant makes core particles containing pgRNA-minus strand DNA hybrid because RNase H activity is blocked by aspartic acid 750. HBV wild type and various mutants were transiently expressed in HuH7 cells and analyzed the intracellular distribution of core particles by immunofluorescence assay and confocal microscopy. From series of replicative stages of core particles, only core particle in late stages of HBV DNA replication with completed minus-strand DNA or relaxed circular DNA genome, can co-localize with microtubule, vimentin and nuclear pore complex of host cells. PART 2 Replication Competent Core Particles of Hepatitis B Virus Were Formed by Chimeric Core Protein Assembly of replication competent HB…
abstract: PART 1 Intracellular Trafficking of HBV Core Particle in Various Stage of DNA Replication Inside of hepatitis B virus (HBV) core particle, pgRNA is reverse transcribed by HBV DNA polymerase into minus-strand DNA and pregenomic RNA (pgRNA) is degraded concomitantly by RNase H activity of DNA polymerase. Then, plus-strand DNA is synthesized to double-stranded relaxed circular DNA. Through this HBV DNA replication, immature core particle becomes mature core particle. This mature core particle can either be transported back into nucleus to amplify the intracellular viral genome pool, or be enveloped at intracellular membrane to be secreted as a mature virion. In this study, intracellular trafficking of HBV core particles was investigated according to the of HBV DNA replication stages. To obtain the core particles, which represent the various stages of HBV DNA replication, several HBV mutants were constructed. RT YMHA, reverse transcription deficient, mutant can encapsidate pgRNA but DNA replication is completely blocked by mutation of YMDD reverse transcriptase motif to YMHA. Priming deficient TP Y65F mutant core particles, in which tyrosine 65 in TP domain of HBV DNA polymerase was changed to phenylalanine, can synthesize oligomer, the nascent minus-strand DNA. RH D750V mutant makes core particles containing pgRNA-minus strand DNA hybrid because RNase H activity is blocked by aspartic acid 750. HBV wild type and various mutants were transiently expressed in HuH7 cells and analyzed the intracellular distribution of core particles by immunofluorescence assay and confocal microscopy. From series of replicative stages of core particles, only core particle in late stages of HBV DNA replication with completed minus-strand DNA or relaxed circular DNA genome, can co-localize with microtubule, vimentin and nuclear pore complex of host cells. PART 2 Replication Competent Core Particles of Hepatitis B Virus Were Formed by Chimeric Core Protein Assembly of replication competent HB…
author: 김, 희영
language: en
keywords: chimeric core protein, dNTP binding cleft, HBV polymerase, hepatitis B virus core particle, hepatitis B virus replication, intracellular trafficking, NTP incorporation, replication stages, B형 간염 바이러스의 복제, B형 간염 바이러스의 캡시드 입자, dNTP 결합부위, HBV DNA 중합효소, NTP 결합, 복제단계, 세포 내 분포, 키메라 캡시드 단백질
date published: 2011-01-27T05:44:06Z
date modified: 2026-07-24T00:51:19Z
provider: Ajou University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ajou University