Development of Rapid Lateral-flow Immunochromatographic Test for the Diagnosis of Chikungunya Virus Infection

BACKGROUND/AIMS: Chikungunya (CHIK) virus is a mosquito-transmitted Alphavirus belonging to family Togaviridae that causes a number of outbreaks in Africa, India, and Southeast Asia with high morbidity. The major clinical symptoms resulting from CHIK virus infection are high fever, arthralgia, myalgia, headache and rash, which are similarly manifested in dengue virus infection. Both CHIK virus and dengue virus are transmitted from human to human mainly by Aedes aegypti. Hence, the differentiation of CHIK virus infection from dengue virus infection is required. Presently, the diagnosis of CHIK virus infection is based on laboratory methods such as hemagglutination inhibition (HI) test, IgM capture ELISA, RT-PCR, and on rare occasions on virus isolation. These tests are all time-consuming, labor intensive, and costly, and require trained staffs and equipments regardless of their accuracy. The purpose of this study was to establish a lateral-flow immunochromatographic test (LF-ICT) ideal for applications as rapid point-of-care test for the diagnosis of acute CHIK virus infection. METHODS: Three CHIK virus structural recombinant proteins, capsid protein and, E1 and E2 envelope glycoproteins, were produced in the baculovirus expression system. Using these recombinant proteins, indirect ELISA and LF-ICT were established for detection of IgM against CHIK virus capsid protein, E1 and E2, respectively. The performance of LF-ICT was then compared with that of indirect ELISA using CHIK virus IgM positive and negative serum samples as well as using dengue virus IgM positive serum samples. RESULTS: The E2 ELISA showed 90.0% sensitivity, 100% specificity, 100% PPV (positive predictive value) and 93.8% NPV (negative predictive value). The E1 ELISA showed 77.5% sensitivity, 100% specificity, 100% PPV and 87.0% NPV and the capsid ELISA showed 87.5% sensitivity, 100% specificity, 100% PPV and 92.3% NPV. The E2 LF-ICT showed 92.5% sensitivity, 100% specificity, 100% PPV and 95.2% NPV. The E1 LF-ICT showed 82.5% sensitivity, 100% specificity, 100% PPV and 89.6% NPV, and the capsid LF-ICT showed 87.5% sensitivity, 100% specificity, 100% PPV and 92.3% NPV. Both LF-ICT and indirect ELISA showed no cross-reactivity with DENV IgM positive sera samples. CONCLUSIONS: Both LF-ICT and indirect ELISA showed 100% specificities and 100% PPV regardless of the applied antigens. Although the performance of capsid LF-ICT was same to that of capsid ELISA, the sensitivity as well as NPV of LF-ICT for detection of IgM against CHIK virus E1 or E2 was superior to that of indirect ELISA. Among the three proteins, E2 showed better performance of immuno-reactivity than E1 or capsid. Thus, the LF-ICT using E2 could be a useful tool for rapid, and differential diagnosis of acute CHIK virus infection from a clinically similar disease like dengue virus.

Author dc:creator

• 조, 병기

Contributors dc:contributor

• 박, 선
• 대학원 의학과
• 200624074

• 치쿤구냐 바이러스
• 배큘로바이러스
• 바이러스 구조적 단백질
• 알파바이러스
• 면역학적 진단

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009135
000000009135

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1304