{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/13044"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/13044","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"Regulation of Astrocyte Functions by a Parkinson's Disease Gene, DJ-1","abstract":"INTRODUCTION . 1 A. Parkinson's disease (PD) 1 B. Genes associated with PD . 2 1. DJ-1 (PARK7) 2 2. Other genes . 3 C. Function of astrocytes in the normal brain . 6 D. Function of astrocytes in the injured brain . 7 1. Reactive astrocytes : intermediate filaments (Ifs) and morphological features . 7 2. Reactive astrocytes : function of astrogliosis in neural protection . 8 3. Reactive astrocytes : function of astrogliosis for regeneration and repair . 9 4. Reactive astorycytes : functin of astrogliosis for regulation of inflammation . 11 E. Astrocyte dysfunction in neurodegenerative disease 12 F. Aim of this study 13 II. Materials and methods 15 1. Ethics statement . 15 2. DJ-1 deficient mice . 15 3. Animal MRI 15 4. Stereotaxic surgery and drug injection . 16 5. Tissue preparation . 16 6. Immunohistochemistry 17 7. Histological quantification 18 8. Organotypic cortical slice cultures 18 9. Cell culture 19 10. Western blot analysis . 19 11. Immunoprecipitation 21 12. Quantitative real-time PCR. 21 13. Reverse transcriptase PCR 22 14. DNA constructs . 23 15. Transfection 23 16. Proximity ligation assay (PLA) 23 17. Small interfering RNAs (siRNA) 24 18. Prostaglandin D2 (PGD2) ELISA . 25 19. Inhibition of protein synthesis by cycloheximide 25 20. Inhibition of Ubiquitination by MG132 25 21. Measurement of nitric oxide 25 22. Statistical analysis . 26 III. RESULTS 27 Part A. DJ-1, (PARK7), plays critical roles in astrogliosis by regulating of the pSTAT3 signaling pathway for tissue repair after brain injury 27 1. Repair of brain injury was attenuated in DJ-1 KO mice 27 2. DJ-1 deficiency causes a defect of astrogliosis . 30 3. Reduction of astrocyte derived GDNF expression and attenuated restoration of dopaminergic processes in the DJ-1 KO brain 41 4. DJ-1 rescued insufficient astrogliosis in DJ-1 KO brain slice cultures. 47 5. STAT3 activation (tyrosine phosphorylation of STAT3, pSTAT3) was attenuated in DJ- 1 KO slices 50 Part B. DJ-1 exerts the anti-inflammatory effects of astrocytes through stabilization of Sox9, a transcription factor that regulates lipocalin-type prostaglandin D2 synthase (L-PGDS) expression. 52 1. DJ-1 deficiency caused a defect of anti-inflammatory function of astrocytes. 52 2. DJ-1 deficiency caused a defect of PGD2 expression by attenuation of L-PGDS in astrocytes. 57 3. DJ-1 deficiency caused a defect of Sox9 protein expression by regulation of ubiquitination in astrocytes. 65 IV. DISCUSSION 73 V. SUMMARY AND CONCLUSION. 85 REFERENCES. 86 국문요약 . 109","abstract_html":"INTRODUCTION . 1 A. Parkinson's disease (PD) 1 B. Genes associated with PD . 2 1. DJ-1 (PARK7) 2 2. Other genes . 3 C. Function of astrocytes in the normal brain . 6 D. Function of astrocytes in the injured brain . 7 1. Reactive astrocytes : intermediate filaments (Ifs) and morphological features . 7 2. Reactive astrocytes : function of astrogliosis in neural protection . 8 3. Reactive astrocytes : function of astrogliosis for regeneration and repair . 9 4. Reactive astorycytes : functin of astrogliosis for regulation of inflammation . 11 E. Astrocyte dysfunction in neurodegenerative disease 12 F. Aim of this study 13 II. Materials and methods 15 1. Ethics statement . 15 2. DJ-1 deficient mice . 15 3. Animal MRI 15 4. Stereotaxic surgery and drug injection . 16 5. Tissue preparation . 16 6. Immunohistochemistry 17 7. Histological quantification 18 8. Organotypic cortical slice cultures 18 9. Cell culture 19 10. Western blot analysis . 19 11. Immunoprecipitation 21 12. Quantitative real-time PCR. 21 13. Reverse transcriptase PCR 22 14. DNA constructs . 23 15. Transfection 23 16. Proximity ligation assay (PLA) 23 17. Small interfering RNAs (siRNA) 24 18. Prostaglandin D2 (PGD2) ELISA . 25 19. Inhibition of protein synthesis by cycloheximide 25 20. Inhibition of Ubiquitination by MG132 25 21. Measurement of nitric oxide 25 22. Statistical analysis . 26 III. RESULTS 27 Part A. DJ-1, (PARK7), plays critical roles in astrogliosis by regulating of the pSTAT3 signaling pathway for tissue repair after brain injury 27 1. Repair of brain injury was attenuated in DJ-1 KO mice 27 2. DJ-1 deficiency causes a defect of astrogliosis . 30 3. Reduction of astrocyte derived GDNF expression and attenuated restoration of dopaminergic processes in the DJ-1 KO brain 41 4. DJ-1 rescued insufficient astrogliosis in DJ-1 KO brain slice cultures. 47 5. STAT3 activation (tyrosine phosphorylation of STAT3, pSTAT3) was attenuated in DJ- 1 KO slices 50 Part B. DJ-1 exerts the anti-inflammatory effects of astrocytes through stabilization of Sox9, a transcription factor that regulates lipocalin-type prostaglandin D2 synthase (L-PGDS) expression. 52 1. DJ-1 deficiency caused a defect of anti-inflammatory function of astrocytes. 52 2. DJ-1 deficiency caused a defect of PGD2 expression by attenuation of L-PGDS in astrocytes. 57 3. DJ-1 deficiency caused a defect of Sox9 protein expression by regulation of ubiquitination in astrocytes. 65 IV. DISCUSSION 73 V. SUMMARY AND CONCLUSION. 85 REFERENCES. 86 국문요약 . 109","abstract_has_math":false,"creators":["최, 동주"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["조, 은혜","대학원 의생명과학과","110942"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-11-29T06:08:37Z","date_published":"2016-11-29T06:08:37Z","updated_at":"2026-07-24T00:51:53Z","subjects":["Parkinsons's disease","DJ-1","Astrocyte","Astrogliosis","Anti-inflammatory function"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000022796","000000022796"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000022796","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000022796","code":true},{"text":"000000022796","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/13044","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["조, 은혜","대학원 의생명과학과","110942","최, 동주"]},{"key":"dc:creator","label":"Author","values":["최, 동주"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-11-29T06:08:37Z","2016"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Parkinsons's disease","DJ-1","Astrocyte","Astrogliosis","Anti-inflammatory function"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/13044","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000022796","000000022796"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["INTRODUCTION . 1 A. Parkinson's disease (PD) 1 B. Genes associated with PD . 2 1. DJ-1 (PARK7) 2 2. Other genes . 3 C. Function of astrocytes in the normal brain . 6 D. Function of astrocytes in the injured brain . 7 1. Reactive astrocytes : intermediate filaments (Ifs) and morphological features . 7 2. Reactive astrocytes : function of astrogliosis in neural protection . 8 3. Reactive astrocytes : function of astrogliosis for regeneration and repair . 9 4. Reactive astorycytes : functin of astrogliosis for regulation of inflammation . 11 E. Astrocyte dysfunction in neurodegenerative disease 12 F. Aim of this study 13 II. Materials and methods 15 1. Ethics statement . 15 2. DJ-1 deficient mice . 15 3. Animal MRI 15 4. Stereotaxic surgery and drug injection . 16 5. Tissue preparation . 16 6. Immunohistochemistry 17 7. Histological quantification 18 8. Organotypic cortical slice cultures 18 9. Cell culture 19 10. Western blot analysis . 19 11. Immunoprecipitation 21 12. Quantitative real-time PCR. 21 13. Reverse transcriptase PCR 22 14. DNA constructs . 23 15. Transfection 23 16. Proximity ligation assay (PLA) 23 17. Small interfering RNAs (siRNA) 24 18. Prostaglandin D2 (PGD2) ELISA . 25 19. Inhibition of protein synthesis by cycloheximide 25 20. Inhibition of Ubiquitination by MG132 25 21. Measurement of nitric oxide 25 22. Statistical analysis . 26 III. RESULTS 27 Part A. DJ-1, (PARK7), plays critical roles in astrogliosis by regulating of the pSTAT3 signaling pathway for tissue repair after brain injury 27 1. Repair of brain injury was attenuated in DJ-1 KO mice 27 2. DJ-1 deficiency causes a defect of astrogliosis . 30 3. Reduction of astrocyte derived GDNF expression and attenuated restoration of dopaminergic processes in the DJ-1 KO brain 41 4. DJ-1 rescued insufficient astrogliosis in DJ-1 KO brain slice cultures. 47 5. STAT3 activation (tyrosine phosphorylation of STAT3, pSTAT3) was attenuated in DJ- 1 KO slices 50 Part B. DJ-1 exerts the anti-inflammatory effects of astrocytes through stabilization of Sox9, a transcription factor that regulates lipocalin-type prostaglandin D2 synthase (L-PGDS) expression. 52 1. DJ-1 deficiency caused a defect of anti-inflammatory function of astrocytes. 52 2. DJ-1 deficiency caused a defect of PGD2 expression by attenuation of L-PGDS in astrocytes. 57 3. DJ-1 deficiency caused a defect of Sox9 protein expression by regulation of ubiquitination in astrocytes. 65 IV. DISCUSSION 73 V. SUMMARY AND CONCLUSION. 85 REFERENCES. 86 국문요약 . 109","Doctor"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Regulation of Astrocyte Functions by a Parkinson's Disease Gene, DJ-1","파킨슨병 유전자 DJ-1에 의한 성상세포 기능 조절 연구"]}]}],"canonical_facts":{"dc:contributor":["조, 은혜","대학원 의생명과학과","110942","최, 동주"],"dc:creator":["최, 동주"],"dc:date":["2016-11-29T06:08:37Z","2016"],"dc:description":["INTRODUCTION . 1 A. Parkinson's disease (PD) 1 B. Genes associated with PD . 2 1. DJ-1 (PARK7) 2 2. Other genes . 3 C. Function of astrocytes in the normal brain . 6 D. Function of astrocytes in the injured brain . 7 1. Reactive astrocytes : intermediate filaments (Ifs) and morphological features . 7 2. Reactive astrocytes : function of astrogliosis in neural protection . 8 3. Reactive astrocytes : function of astrogliosis for regeneration and repair . 9 4. Reactive astorycytes : functin of astrogliosis for regulation of inflammation . 11 E. Astrocyte dysfunction in neurodegenerative disease 12 F. Aim of this study 13 II. Materials and methods 15 1. Ethics statement . 15 2. DJ-1 deficient mice . 15 3. Animal MRI 15 4. Stereotaxic surgery and drug injection . 16 5. Tissue preparation . 16 6. Immunohistochemistry 17 7. Histological quantification 18 8. Organotypic cortical slice cultures 18 9. Cell culture 19 10. Western blot analysis . 19 11. Immunoprecipitation 21 12. Quantitative real-time PCR. 21 13. Reverse transcriptase PCR 22 14. DNA constructs . 23 15. Transfection 23 16. Proximity ligation assay (PLA) 23 17. Small interfering RNAs (siRNA) 24 18. Prostaglandin D2 (PGD2) ELISA . 25 19. Inhibition of protein synthesis by cycloheximide 25 20. Inhibition of Ubiquitination by MG132 25 21. Measurement of nitric oxide 25 22. Statistical analysis . 26 III. RESULTS 27 Part A. DJ-1, (PARK7), plays critical roles in astrogliosis by regulating of the pSTAT3 signaling pathway for tissue repair after brain injury 27 1. Repair of brain injury was attenuated in DJ-1 KO mice 27 2. DJ-1 deficiency causes a defect of astrogliosis . 30 3. Reduction of astrocyte derived GDNF expression and attenuated restoration of dopaminergic processes in the DJ-1 KO brain 41 4. DJ-1 rescued insufficient astrogliosis in DJ-1 KO brain slice cultures. 47 5. STAT3 activation (tyrosine phosphorylation of STAT3, pSTAT3) was attenuated in DJ- 1 KO slices 50 Part B. DJ-1 exerts the anti-inflammatory effects of astrocytes through stabilization of Sox9, a transcription factor that regulates lipocalin-type prostaglandin D2 synthase (L-PGDS) expression. 52 1. DJ-1 deficiency caused a defect of anti-inflammatory function of astrocytes. 52 2. DJ-1 deficiency caused a defect of PGD2 expression by attenuation of L-PGDS in astrocytes. 57 3. DJ-1 deficiency caused a defect of Sox9 protein expression by regulation of ubiquitination in astrocytes. 65 IV. DISCUSSION 73 V. SUMMARY AND CONCLUSION. 85 REFERENCES. 86 국문요약 . 109","Doctor"],"dc:format":["application/pdf"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/13044","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000022796","000000022796"],"dc:language":["en"],"dc:subject":["Parkinsons's disease","DJ-1","Astrocyte","Astrogliosis","Anti-inflammatory function"],"dc:title":["Regulation of Astrocyte Functions by a Parkinson's Disease Gene, DJ-1","파킨슨병 유전자 DJ-1에 의한 성상세포 기능 조절 연구"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:53Z"}