Bridging the lesioned spinal cord using hydrogel
I. INTRODUCTION 1 A. Spinal Cord Injury 1 B. Cavity formation and the importance of bridging lesion after spinal cord injury. 2 C. Bridging cavity using biomaterials 3 D. Injectable hydrogel for contusive injury model 4 E. Aims of Study 5 II. MATERIALS AND METHODS 6 1. Animal and surgical procedures 6 2. Poly (phosphazene) hydrogel injection 6 3. Tissue processing 6 4. Immunohistochemistry 7 5. Three dimensional reconstruction of lesion cavity 7 6. Zymography 7 7. Behavioral assessment 8 III. RESULTS 10 1. Hydrogel injection prevent cavity formation after contusive spinal cord injury 10 2. Hydrogel injection suppress microglial activation 4 week after injection 14 3. Remodelling of extracellular matrix by I-5 injection 16 4. Matrixmetallo-proteinase-9 ( MMP-9) involved in ECM remodelling 20 5. Perivascular fibroblasts are a major source of the newly formed ECM at 1 and 4 week 23 6. I-5 injection promotes functional recovery 25 IV. DISCUSSION 28 V. CONCLUSION 31
Spinal cord injury, hydrogel, extracullular matrix
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