{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/11835"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/11835","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"Bridging the lesioned spinal cord using hydrogel","abstract":"I. INTRODUCTION 1 A. Spinal Cord Injury 1 B. Cavity formation and the importance of bridging lesion after spinal cord injury. 2 C. Bridging cavity using biomaterials 3 D. Injectable hydrogel for contusive injury model 4 E. Aims of Study 5 II. MATERIALS AND METHODS 6 1. Animal and surgical procedures 6 2. Poly (phosphazene) hydrogel injection 6 3. Tissue processing 6 4. Immunohistochemistry 7 5. Three dimensional reconstruction of lesion cavity 7 6. Zymography 7 7. Behavioral assessment 8 III. RESULTS 10 1. Hydrogel injection prevent cavity formation after contusive spinal cord injury 10 2. Hydrogel injection suppress microglial activation 4 week after injection 14 3. Remodelling of extracellular matrix by I-5 injection 16 4. Matrixmetallo-proteinase-9 ( MMP-9) involved in ECM remodelling 20 5. Perivascular fibroblasts are a major source of the newly formed ECM at 1 and 4 week 23 6. I-5 injection promotes functional recovery 25 IV. DISCUSSION 28 V. CONCLUSION 31","abstract_html":"I. INTRODUCTION 1 A. Spinal Cord Injury 1 B. Cavity formation and the importance of bridging lesion after spinal cord injury. 2 C. Bridging cavity using biomaterials 3 D. Injectable hydrogel for contusive injury model 4 E. Aims of Study 5 II. MATERIALS AND METHODS 6 1. Animal and surgical procedures 6 2. Poly (phosphazene) hydrogel injection 6 3. Tissue processing 6 4. Immunohistochemistry 7 5. Three dimensional reconstruction of lesion cavity 7 6. Zymography 7 7. Behavioral assessment 8 III. RESULTS 10 1. Hydrogel injection prevent cavity formation after contusive spinal cord injury 10 2. Hydrogel injection suppress microglial activation 4 week after injection 14 3. Remodelling of extracellular matrix by I-5 injection 16 4. Matrixmetallo-proteinase-9 ( MMP-9) involved in ECM remodelling 20 5. Perivascular fibroblasts are a major source of the newly formed ECM at 1 and 4 week 23 6. I-5 injection promotes functional recovery 25 IV. DISCUSSION 28 V. CONCLUSION 31","abstract_has_math":false,"creators":["Hong, Le Thi Anh"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["김, 병곤","대학원 의생명과학과","201225142"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-10-28T02:56:30Z","date_published":"2015-10-28T02:56:30Z","updated_at":"2026-07-24T00:51:50Z","subjects":["Spinal cord injury","hydrogel","extracullular matrix"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020728","000000020728"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020728","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020728","code":true},{"text":"000000020728","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/11835","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["김, 병곤","대학원 의생명과학과","201225142","Hong, Le Thi Anh"]},{"key":"dc:creator","label":"Author","values":["Hong, Le Thi Anh"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-10-28T02:56:30Z","2015"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Spinal cord injury","hydrogel","extracullular matrix"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/11835","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020728","000000020728"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["I. INTRODUCTION 1 A. Spinal Cord Injury 1 B. Cavity formation and the importance of bridging lesion after spinal cord injury. 2 C. Bridging cavity using biomaterials 3 D. Injectable hydrogel for contusive injury model 4 E. Aims of Study 5 II. MATERIALS AND METHODS 6 1. Animal and surgical procedures 6 2. Poly (phosphazene) hydrogel injection 6 3. Tissue processing 6 4. Immunohistochemistry 7 5. Three dimensional reconstruction of lesion cavity 7 6. Zymography 7 7. Behavioral assessment 8 III. RESULTS 10 1. Hydrogel injection prevent cavity formation after contusive spinal cord injury 10 2. Hydrogel injection suppress microglial activation 4 week after injection 14 3. Remodelling of extracellular matrix by I-5 injection 16 4. Matrixmetallo-proteinase-9 ( MMP-9) involved in ECM remodelling 20 5. Perivascular fibroblasts are a major source of the newly formed ECM at 1 and 4 week 23 6. I-5 injection promotes functional recovery 25 IV. DISCUSSION 28 V. CONCLUSION 31","Master"]},{"key":"dc:format","label":"Dc Format","values":["text/plain"]},{"key":"dc:title","label":"Title","values":["Bridging the lesioned spinal cord using hydrogel"]}]}],"canonical_facts":{"dc:contributor":["김, 병곤","대학원 의생명과학과","201225142","Hong, Le Thi Anh"],"dc:creator":["Hong, Le Thi Anh"],"dc:date":["2015-10-28T02:56:30Z","2015"],"dc:description":["I. INTRODUCTION 1 A. Spinal Cord Injury 1 B. Cavity formation and the importance of bridging lesion after spinal cord injury. 2 C. Bridging cavity using biomaterials 3 D. Injectable hydrogel for contusive injury model 4 E. Aims of Study 5 II. MATERIALS AND METHODS 6 1. Animal and surgical procedures 6 2. Poly (phosphazene) hydrogel injection 6 3. Tissue processing 6 4. Immunohistochemistry 7 5. Three dimensional reconstruction of lesion cavity 7 6. Zymography 7 7. Behavioral assessment 8 III. RESULTS 10 1. Hydrogel injection prevent cavity formation after contusive spinal cord injury 10 2. Hydrogel injection suppress microglial activation 4 week after injection 14 3. Remodelling of extracellular matrix by I-5 injection 16 4. Matrixmetallo-proteinase-9 ( MMP-9) involved in ECM remodelling 20 5. Perivascular fibroblasts are a major source of the newly formed ECM at 1 and 4 week 23 6. I-5 injection promotes functional recovery 25 IV. DISCUSSION 28 V. CONCLUSION 31","Master"],"dc:format":["text/plain"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/11835","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020728","000000020728"],"dc:language":["en"],"dc:subject":["Spinal cord injury","hydrogel","extracullular matrix"],"dc:title":["Bridging the lesioned spinal cord using hydrogel"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:50Z"}