{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/11827"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/11827","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"CDDO-ME induces apoptosis in breast cancer cells via Ca2+ influx-mediated dilation of endoplasmic reticulum and c-FLIP downregulation","abstract":"I.INTRODUCTION 1 II.MATERIALS AND METHODS 6 A.Chemicals and antibodies 6 B.Cell culture of various cancer cell lines 7 C.Measurement of cell viability 7 D.Western blotting 7 E.Immunocytochemistry 8 F.Establishment of the stable cell lines in the fluorescence specifically mitochondria or endoplasmic reticulum 9 G.Measurement of ROS and mitochondrial superoxide anion 9 H.Measurement of cytosolic and mitochondrial Ca² levels 9 I.shRNA-mediated knockdown of proteins 10 J.Transmission electron microscopy 10 K.Reverse transcriptionPCR analysis 11 L.Statistical analysis 12 III. RESULTS 13 1.CDDO-ME demonstrates a potent anti-cancer effect on breast cancer cells 13 2.CDDO-ME induces paraptosis-like cellular vacuolation prior to morphologies features of apoptosis in breast cancer cells 17 3.Ca2+ influx is crucial for CDDO-ME-induced vacuolation and subsequent apoptotic cell death 40 4.Cross-modulation between Ca2+ influx and ROS generation critically contributes to CDDO-ME-induced vacuolation and subsequent apoptosis 51 5.c-FLIPL downregulation plays a critical role in CDDO-ME-induced apoptotic cell death, but not in vacuolation 58 6.Higher increase in intracellular Ca2+ and ROS levels as well as c-FLIP downregulation may contribute to a more potent anti-cancer effect of CDDO-ME, compared to CDDO 69 IV.DISCUSSION 73 V.REFERENCES 79 -국문요약- 90","abstract_html":"I.INTRODUCTION 1 II.MATERIALS AND METHODS 6 A.Chemicals and antibodies 6 B.Cell culture of various cancer cell lines 7 C.Measurement of cell viability 7 D.Western blotting 7 E.Immunocytochemistry 8 F.Establishment of the stable cell lines in the fluorescence specifically mitochondria or endoplasmic reticulum 9 G.Measurement of ROS and mitochondrial superoxide anion 9 H.Measurement of cytosolic and mitochondrial Ca² levels 9 I.shRNA-mediated knockdown of proteins 10 J.Transmission electron microscopy 10 K.Reverse transcriptionPCR analysis 11 L.Statistical analysis 12 III. RESULTS 13 1.CDDO-ME demonstrates a potent anti-cancer effect on breast cancer cells 13 2.CDDO-ME induces paraptosis-like cellular vacuolation prior to morphologies features of apoptosis in breast cancer cells 17 3.Ca2+ influx is crucial for CDDO-ME-induced vacuolation and subsequent apoptotic cell death 40 4.Cross-modulation between Ca2+ influx and ROS generation critically contributes to CDDO-ME-induced vacuolation and subsequent apoptosis 51 5.c-FLIPL downregulation plays a critical role in CDDO-ME-induced apoptotic cell death, but not in vacuolation 58 6.Higher increase in intracellular Ca2+ and ROS levels as well as c-FLIP downregulation may contribute to a more potent anti-cancer effect of CDDO-ME, compared to CDDO 69 IV.DISCUSSION 73 V.REFERENCES 79 -국문요약- 90","abstract_has_math":false,"creators":["정, 수아"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["최, 경숙","대학원 의생명과학과","201324121"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-10-28","date_published":"2015-10-28","updated_at":"2026-07-24T00:51:50Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000019228","000000019228"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000019228","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000019228","code":true},{"text":"000000019228","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/11827","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["최, 경숙","대학원 의생명과학과","201324121","정, 수아"]},{"key":"dc:creator","label":"Author","values":["정, 수아"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-10-28","2015"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/11827","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000019228","000000019228"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["I.INTRODUCTION 1 II.MATERIALS AND METHODS 6 A.Chemicals and antibodies 6 B.Cell culture of various cancer cell lines 7 C.Measurement of cell viability 7 D.Western blotting 7 E.Immunocytochemistry 8 F.Establishment of the stable cell lines in the fluorescence specifically mitochondria or endoplasmic reticulum 9 G.Measurement of ROS and mitochondrial superoxide anion 9 H.Measurement of cytosolic and mitochondrial Ca² levels 9 I.shRNA-mediated knockdown of proteins 10 J.Transmission electron microscopy 10 K.Reverse transcriptionPCR analysis 11 L.Statistical analysis 12 III. RESULTS 13 1.CDDO-ME demonstrates a potent anti-cancer effect on breast cancer cells 13 2.CDDO-ME induces paraptosis-like cellular vacuolation prior to morphologies features of apoptosis in breast cancer cells 17 3.Ca2+ influx is crucial for CDDO-ME-induced vacuolation and subsequent apoptotic cell death 40 4.Cross-modulation between Ca2+ influx and ROS generation critically contributes to CDDO-ME-induced vacuolation and subsequent apoptosis 51 5.c-FLIPL downregulation plays a critical role in CDDO-ME-induced apoptotic cell death, but not in vacuolation 58 6.Higher increase in intracellular Ca2+ and ROS levels as well as c-FLIP downregulation may contribute to a more potent anti-cancer effect of CDDO-ME, compared to CDDO 69 IV.DISCUSSION 73 V.REFERENCES 79 -국문요약- 90","Master"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["CDDO-ME induces apoptosis in breast cancer cells via Ca2+ influx-mediated dilation of endoplasmic reticulum and c-FLIP downregulation","악성 유방암 세포에서 CDDO-ME 처리 시 Ca2+ 유입에 매개된 소포체 팽창과 c-FLIP 감소를 통한 세포자살 유도"]}]}],"canonical_facts":{"dc:contributor":["최, 경숙","대학원 의생명과학과","201324121","정, 수아"],"dc:creator":["정, 수아"],"dc:date":["2015-10-28","2015"],"dc:description":["I.INTRODUCTION 1 II.MATERIALS AND METHODS 6 A.Chemicals and antibodies 6 B.Cell culture of various cancer cell lines 7 C.Measurement of cell viability 7 D.Western blotting 7 E.Immunocytochemistry 8 F.Establishment of the stable cell lines in the fluorescence specifically mitochondria or endoplasmic reticulum 9 G.Measurement of ROS and mitochondrial superoxide anion 9 H.Measurement of cytosolic and mitochondrial Ca² levels 9 I.shRNA-mediated knockdown of proteins 10 J.Transmission electron microscopy 10 K.Reverse transcriptionPCR analysis 11 L.Statistical analysis 12 III. RESULTS 13 1.CDDO-ME demonstrates a potent anti-cancer effect on breast cancer cells 13 2.CDDO-ME induces paraptosis-like cellular vacuolation prior to morphologies features of apoptosis in breast cancer cells 17 3.Ca2+ influx is crucial for CDDO-ME-induced vacuolation and subsequent apoptotic cell death 40 4.Cross-modulation between Ca2+ influx and ROS generation critically contributes to CDDO-ME-induced vacuolation and subsequent apoptosis 51 5.c-FLIPL downregulation plays a critical role in CDDO-ME-induced apoptotic cell death, but not in vacuolation 58 6.Higher increase in intracellular Ca2+ and ROS levels as well as c-FLIP downregulation may contribute to a more potent anti-cancer effect of CDDO-ME, compared to CDDO 69 IV.DISCUSSION 73 V.REFERENCES 79 -국문요약- 90","Master"],"dc:format":["application/pdf"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/11827","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000019228","000000019228"],"dc:language":["en"],"dc:title":["CDDO-ME induces apoptosis in breast cancer cells via Ca2+ influx-mediated dilation of endoplasmic reticulum and c-FLIP downregulation","악성 유방암 세포에서 CDDO-ME 처리 시 Ca2+ 유입에 매개된 소포체 팽창과 c-FLIP 감소를 통한 세포자살 유도"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:50Z"}