Investigation on the Molecular Mechanism of Photorejuvenation in Photodynamic Therapy
type: CreativeWork
description: BACKGROUND: The photoaging of the skin is characterized by wrinkles, mottled pigmentation, telangiectasia and malignancies due to the effects of long-term UV exposure. Photodynamic therapy (PDT) is a therapeutic modality for cutaneous malignant and premalignant condition. Recently, several clinical studies reported that the PDT has photorejuvenation effects on the aged skin. In the previous report, which is examined direct effect of PDT on fibroblast (FB), PDT induced increase of matrix metalloproteinase (MMP)-3 and collagen type Iα through prolonged activation of extracellular signal–regulated kinase (ERK). Increased MMP-3 may induce breakage of old collagen and allow new collagen synthesis. PURPOSE: In this study, I investigated molecular mechanism of photorejuvenation after PDT focused on interaction between keratinocyte (KC), FB, and melanocyte (MC). METHODS: The “low level PDT” condition was used for PDT therapy to the KC, FB, and MC. FBs were stimulated with the KC-conditioned medium taken after PDT and MCs were stimulated with the KC-conditioned medium or FB-conditioned medium taken after PDT. Various kinds of cytokines in the supernatants of KC and FB were evaluated by enzymelinked immunosorbent assay (ELISA). The mRNA level of MMPs, transforming growth factor (TGF)-β and collagen type Iα in the FB were determined by real-time polymerase chain reaction (PCR). The melanin content and dopa oxidase activity of the MC were also evaluated. RESULTS: After stimulating FB with KC-conditioned medium, the mRNA of MMP-1 was decreased and the mRNA of collagen type Iα were increased compare to control. However, MMP-3 and TGF-β were not significantly changed. Among the FB stimulating cytokines, a significant elevation of interleukin (IL)-1α, IL-6, tumor necrosis factor (TNF)-α level in KC supernatants was noted after PDT compared with the untreated controls. TGF-β were not significantly altered after PDT. After PDT, significantly reduced melanin content and dopa oxidase a…
abstract: BACKGROUND: The photoaging of the skin is characterized by wrinkles, mottled pigmentation, telangiectasia and malignancies due to the effects of long-term UV exposure. Photodynamic therapy (PDT) is a therapeutic modality for cutaneous malignant and premalignant condition. Recently, several clinical studies reported that the PDT has photorejuvenation effects on the aged skin. In the previous report, which is examined direct effect of PDT on fibroblast (FB), PDT induced increase of matrix metalloproteinase (MMP)-3 and collagen type Iα through prolonged activation of extracellular signal–regulated kinase (ERK). Increased MMP-3 may induce breakage of old collagen and allow new collagen synthesis. PURPOSE: In this study, I investigated molecular mechanism of photorejuvenation after PDT focused on interaction between keratinocyte (KC), FB, and melanocyte (MC). METHODS: The “low level PDT” condition was used for PDT therapy to the KC, FB, and MC. FBs were stimulated with the KC-conditioned medium taken after PDT and MCs were stimulated with the KC-conditioned medium or FB-conditioned medium taken after PDT. Various kinds of cytokines in the supernatants of KC and FB were evaluated by enzymelinked immunosorbent assay (ELISA). The mRNA level of MMPs, transforming growth factor (TGF)-β and collagen type Iα in the FB were determined by real-time polymerase chain reaction (PCR). The melanin content and dopa oxidase activity of the MC were also evaluated. RESULTS: After stimulating FB with KC-conditioned medium, the mRNA of MMP-1 was decreased and the mRNA of collagen type Iα were increased compare to control. However, MMP-3 and TGF-β were not significantly changed. Among the FB stimulating cytokines, a significant elevation of interleukin (IL)-1α, IL-6, tumor necrosis factor (TNF)-α level in KC supernatants was noted after PDT compared with the untreated controls. TGF-β were not significantly altered after PDT. After PDT, significantly reduced melanin content and dopa oxidase a…
author: 김, 수경
language: en
keywords: Photodynamic therapy, Photorejuvenation, Keratinocyte, Fibroblast, Melanocyte, 광역동 치료, 광회춘, 각질세포, 섬유아세포, 멜라닌세포
date published: 2014-11-13T01:45:14Z
date modified: 2026-07-24T00:51:47Z
provider: Ajou University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ajou University