Microbiology and cell biology of the interaction between Listeria monocytogenes and Acanthamoeba spp.
type: CreativeWork
description: L. monocytogenes is ubiquitous in environment and can grow and survive in a wide range of environmental conditions. It contaminates foods via raw materials or food processing environments. However, the current knowledge of its ecology and in particular, the mode of environmental survival and transmission of L. monocytogenes remains limited. One important aspect of environmental survival of L. monocytogenes may be contact with other microorganisms, including amoebae, which naturally feed on bacteria as a source of nutrients. In this context, research has shown that several intra-cellular pathogens are able to survive or replicate within free-living amoebae. In view of the potential for amoebae to act as environmental reservoirs for bacteria, the interaction of L. monocytogenes with freeliving Acanthamoeba spp. and the impact of plasmid-associated genes on their interaction were investigated. Several strains of environmental and clinical isolates of L. monocytogenes were used for co-culture with amoebae. Axenic amoebae were isolated from environmental sources (water, soil) by cultivation in PYG supplemented with antibiotics. L. monocytogenes strains were co-cultured with amoebae on plates, in trays (as monolayers of amoeba cells) and in flasks to provide qualitative and quantitative assessments of the survival of bacteria and amoebae. Bacteriological methods and microscopy (fluorescence, TEM and phase contrast) were used to track the fate of internalized bacteria. A vector that allowed GFP expression under control of the prfA promoter was used to assess the expression of Listeria virulence genes within amoeba cells. The role plasmid encoded genes in interactions of L. monocytogenes with amoebae was assessed using plasmid-cured versus wild type in the co-cultures. Finally the mechanisms of bacterial uptake and killing by A. polyphaga were assessed using chemical inhibitors that affected actin polymerization (Cytochalasin D, Wortmannin), phagosome-lysosome fusion (Suram…
abstract: L. monocytogenes is ubiquitous in environment and can grow and survive in a wide range of environmental conditions. It contaminates foods via raw materials or food processing environments. However, the current knowledge of its ecology and in particular, the mode of environmental survival and transmission of L. monocytogenes remains limited. One important aspect of environmental survival of L. monocytogenes may be contact with other microorganisms, including amoebae, which naturally feed on bacteria as a source of nutrients. In this context, research has shown that several intra-cellular pathogens are able to survive or replicate within free-living amoebae. In view of the potential for amoebae to act as environmental reservoirs for bacteria, the interaction of L. monocytogenes with freeliving Acanthamoeba spp. and the impact of plasmid-associated genes on their interaction were investigated. Several strains of environmental and clinical isolates of L. monocytogenes were used for co-culture with amoebae. Axenic amoebae were isolated from environmental sources (water, soil) by cultivation in PYG supplemented with antibiotics. L. monocytogenes strains were co-cultured with amoebae on plates, in trays (as monolayers of amoeba cells) and in flasks to provide qualitative and quantitative assessments of the survival of bacteria and amoebae. Bacteriological methods and microscopy (fluorescence, TEM and phase contrast) were used to track the fate of internalized bacteria. A vector that allowed GFP expression under control of the prfA promoter was used to assess the expression of Listeria virulence genes within amoeba cells. The role plasmid encoded genes in interactions of L. monocytogenes with amoebae was assessed using plasmid-cured versus wild type in the co-cultures. Finally the mechanisms of bacterial uptake and killing by A. polyphaga were assessed using chemical inhibitors that affected actin polymerization (Cytochalasin D, Wortmannin), phagosome-lysosome fusion (Suram…
author: Akya, Alisha
keywords: microbiology; cell biology; Listeria monocytogenes; Acanthamoeba; co-culture
date published: 2007
date modified: 2026-07-24T00:50:50Z
provider: University of Adelaide
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Adelaide

PropertyValue
property ID: Handle
value: http://hdl.handle.net/2440/47536