The relationship of epithelial rests of Malassez to orthodontic resorption lacunae: a three dimensional reconstruction from serial sections

Uncertainty as to the role of the epithelial remnants from cementogenesis has been discussed in the literature since these cells were first described over one hundred years ago. Among the possible functions suggested has been a role in healing of root resorption. This study has been undertaken to establish an economical technique for producing useful three dimensional reconstructions of human periodontal ligament and attempts to determine the relationship of epithelial rests of Malassez with orthodontic root resorption to clarify any potential function. Root resorption was produced on the buccal surfaces of human premolars following rapid maxillary expansion therapy. These teeth were extracted and processed for both electron and light microscopy. The work of Brice (1988) found conclusively that epithelial cells are found in repairing resorption lacunae from his ultrastrutural investigations. This work added credence to the belief that epithelial cells may play a role in protecting the tooth from, or at least limiting the progress of root resorption by initiating cementogenesis. The present study involved reconstructing 1 micron serial sections of human periodontal ligament with 3-D computer programmes. These reconstructions enabled a protocol for the production of serial sections of human periodontal ligament to be established. Digitized sections were processed on two independant IBM based reconstruction programmes to determine the most visually useful image system for interpretation. The electron micrographs and three dimensional images produced confirmed the presence of epithelial cells in the proximity of regions of active and repairing resorption. The continuity of these cells was confirmed, and their relationship to blood vessels and tooth described in regions with different degrees of resorptive activity. The possibility of epithelial cells in limiting the degree of root resorption is discussed. Technical difficulties in producing computer generated three dimensional images were discussed and future directions outlined.

Author dc:creator

• Kittel, Paul

Advisor dc:contributor.advisor

• Sampson, WJ

Language dc:language.iso

en

Handle dc:identifier.uri

http://hdl.handle.net/2440/122345

OAI identifier oai:identifier

oai:digital.library.adelaide.edu.au:2440/122345