{"id":{"repo_id":"abertay","oai_identifier":"oai:rke.abertay.ac.uk:studenttheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87"},"canonical_url":"https://search.dev.ndltd.org/etd/abertay/oai:rke.abertay.ac.uk:studenttheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87","repository":{"repo_id":"abertay","name":"Abertay University","base_url":"https://rke.abertay.ac.uk/ws/oai"},"display":{"title":"Oxidative stress and seed survival","abstract":"Free radical and aldehydic breakdown product content were determined, by EPR and UV / visible spectroscopy, primarily in intermediate (desiccation tolerant) seeds of Carica papaya L. (Papaya) and recalcitrant (desiccation intolerant) seeds of Aesculus hippocastanum L. (Horse chestnut), but also in other species covering a range of desiccation tolerances, with a view to determining the role of oxidative stress as a diagnostic marker for desiccation tolerance.
Axes of non-senescent highly viable recalcitrant seeds of horse chestnut were metabolically active, contained products of lipid peroxidation, displayed low levels of enzymatic protection against activated oxygen and peroxides, and a two-peak free radical EPR signal. During fully hydrated storage at 16 °C for up to 18 months, seeds exhibited, sequentially, an increase in germination rate, a transient increase in intensities of both the low field and high field EPR peaks, a significant increase in membrane leakage and decrease in seed viability, germination rate, and SOD and peroxidase activities. Drying ‘unstored’ seeds below and embryonic axis moisture content of 40 to 50 % initiated viability loss. At < 25 % moisture content all axes were inviable and displayed a 2- to 4-fold increase in solute leakage, lipid peroxidation products and the low field EPR signal. Seed desiccation sensitivity increased with hydrated storage. The accumulation of lipid peroxidation products and free radicals on drying generally occurred to a greater extent, or at a higher moisture content, than observed with unstored seeds. The results indicate a mediating role for oxidative stress in recalcitrant seed viability loss which is differentially expressed during hydrated, ‘natural’ ageing and desiccation. Similar trends were seen in other recalcitrant species with the increase in lipid peroxidation products occurring around the point of viability loss. However the study of a more orthodox species (papaya) revealed no such trends.","abstract_html":"Free radical and aldehydic breakdown product content were determined, by EPR and UV / visible spectroscopy, primarily in intermediate (desiccation tolerant) seeds of <i>Carica papaya</i> L. (Papaya) and recalcitrant (desiccation intolerant) seeds of <i>Aesculus hippocastanum</i> L. (Horse chestnut), but also in other species covering a range of desiccation tolerances, with a view to determining the role of oxidative stress as a diagnostic marker for desiccation tolerance.<br/>Axes of non-senescent highly viable recalcitrant seeds of horse chestnut were metabolically active, contained products of lipid peroxidation, displayed low levels of enzymatic protection against activated oxygen and peroxides, and a two-peak free radical EPR signal. During fully hydrated storage at 16 °C for up to 18 months, seeds exhibited, sequentially, an increase in germination rate, a transient increase in intensities of both the low field and high field EPR peaks, a significant increase in membrane leakage and decrease in seed viability, germination rate, and SOD and peroxidase activities. Drying ‘unstored’ seeds below and embryonic axis moisture content of 40 to 50 % initiated viability loss. At < 25 % moisture content all axes were inviable and displayed a 2- to 4-fold increase in solute leakage, lipid peroxidation products and the low field EPR signal. Seed desiccation sensitivity increased with hydrated storage. The accumulation of lipid peroxidation products and free radicals on drying generally occurred to a greater extent, or at a higher moisture content, than observed with unstored seeds. The results indicate a mediating role for oxidative stress in recalcitrant seed viability loss which is differentially expressed during hydrated, ‘natural’ ageing and desiccation. Similar trends were seen in other recalcitrant species with the increase in lipid peroxidation products occurring around the point of viability loss. However the study of a more orthodox species (papaya) revealed no such trends.","abstract_has_math":false,"creators":["Wood, Christopher"],"institution":"University of Abertay Dundee","degree_name":"PhD","degree_level":"Doctoral Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1998,"date_issued":"1998-10","date_published":"1998-10","updated_at":"2026-07-24T00:50:16Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:rke.abertay.ac.uk:studenttheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87"],"render_values":[{"text":"oai:rke.abertay.ac.uk:studenttheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87","href":null,"code":true}]}]},"links":{"outbound_url":"https://rke.abertay.ac.uk/en/studentTheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Wood, Christopher"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1998-10"]},{"key":"dc:date.issued","label":"Date","values":["1998-10"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["University of Abertay Dundee"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Abertay Dundee"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://rke.abertay.ac.uk/en/studentTheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral Thesis"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["PhD"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:rke.abertay.ac.uk:studenttheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87","https://rke.abertay.ac.uk/en/studentTheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://rke.abertay.ac.uk/files/15621846/Wood_1998_Oxidative_stress_and_seed_survival_Redacted.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Free radical and aldehydic breakdown product content were determined, by EPR and UV / visible spectroscopy, primarily in intermediate (desiccation tolerant) seeds of Carica papaya L. (Papaya) and recalcitrant (desiccation intolerant) seeds of Aesculus hippocastanum L. (Horse chestnut), but also in other species covering a range of desiccation tolerances, with a view to determining the role of oxidative stress as a diagnostic marker for desiccation tolerance.
Axes of non-senescent highly viable recalcitrant seeds of horse chestnut were metabolically active, contained products of lipid peroxidation, displayed low levels of enzymatic protection against activated oxygen and peroxides, and a two-peak free radical EPR signal. During fully hydrated storage at 16 °C for up to 18 months, seeds exhibited, sequentially, an increase in germination rate, a transient increase in intensities of both the low field and high field EPR peaks, a significant increase in membrane leakage and decrease in seed viability, germination rate, and SOD and peroxidase activities. Drying ‘unstored’ seeds below and embryonic axis moisture content of 40 to 50 % initiated viability loss. At < 25 % moisture content all axes were inviable and displayed a 2- to 4-fold increase in solute leakage, lipid peroxidation products and the low field EPR signal. Seed desiccation sensitivity increased with hydrated storage. The accumulation of lipid peroxidation products and free radicals on drying generally occurred to a greater extent, or at a higher moisture content, than observed with unstored seeds. The results indicate a mediating role for oxidative stress in recalcitrant seed viability loss which is differentially expressed during hydrated, ‘natural’ ageing and desiccation. Similar trends were seen in other recalcitrant species with the increase in lipid peroxidation products occurring around the point of viability loss. However the study of a more orthodox species (papaya) revealed no such trends."]},{"key":"dc:title","label":"Title","values":["Oxidative stress and seed survival"]}]}],"canonical_facts":{"dc:creator":["Wood, Christopher"],"dc:date":["1998-10"],"dc:date.issued":["1998-10"],"dc:description.abstract":["Free radical and aldehydic breakdown product content were determined, by EPR and UV / visible spectroscopy, primarily in intermediate (desiccation tolerant) seeds of Carica papaya L. (Papaya) and recalcitrant (desiccation intolerant) seeds of Aesculus hippocastanum L. (Horse chestnut), but also in other species covering a range of desiccation tolerances, with a view to determining the role of oxidative stress as a diagnostic marker for desiccation tolerance.
Axes of non-senescent highly viable recalcitrant seeds of horse chestnut were metabolically active, contained products of lipid peroxidation, displayed low levels of enzymatic protection against activated oxygen and peroxides, and a two-peak free radical EPR signal. During fully hydrated storage at 16 °C for up to 18 months, seeds exhibited, sequentially, an increase in germination rate, a transient increase in intensities of both the low field and high field EPR peaks, a significant increase in membrane leakage and decrease in seed viability, germination rate, and SOD and peroxidase activities. Drying ‘unstored’ seeds below and embryonic axis moisture content of 40 to 50 % initiated viability loss. At < 25 % moisture content all axes were inviable and displayed a 2- to 4-fold increase in solute leakage, lipid peroxidation products and the low field EPR signal. Seed desiccation sensitivity increased with hydrated storage. The accumulation of lipid peroxidation products and free radicals on drying generally occurred to a greater extent, or at a higher moisture content, than observed with unstored seeds. The results indicate a mediating role for oxidative stress in recalcitrant seed viability loss which is differentially expressed during hydrated, ‘natural’ ageing and desiccation. Similar trends were seen in other recalcitrant species with the increase in lipid peroxidation products occurring around the point of viability loss. However the study of a more orthodox species (papaya) revealed no such trends."],"dc:identifier":["oai:rke.abertay.ac.uk:studenttheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87","https://rke.abertay.ac.uk/en/studentTheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87"],"dc:identifier.uri":["https://rke.abertay.ac.uk/files/15621846/Wood_1998_Oxidative_stress_and_seed_survival_Redacted.pdf"],"dc:language":["eng"],"dc:publisher.department":["University of Abertay Dundee"],"dc:publisher.institution":["University of Abertay Dundee"],"dc:relation.isreferencedby":["https://rke.abertay.ac.uk/en/studentTheses/79d28b74-9210-4ebd-a3b8-66a610bd8c87"],"dc:title":["Oxidative stress and seed survival"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral Thesis"],"dc:type.qualificationname":["PhD"]},"updated_at":"2026-07-24T00:50:16Z"}