Interactions of human primary immune cells with nanoparticles, two-dimensional micropatterns, hydrogels and three-dimensional nanofibres
type: CreativeWork
description: The response of immune cells to any biomaterial determines its biocompatibility. This study aimed to identify the immunomodulatory properties of biomaterials from four different fields exhibiting various chemical, geometrical and morphological properties: nanoparticles, two-dimensional micropatterned structures, hydrogel with functional endgroups and three-dimensional nanofibers. It was shown in how far chemical, topographical and morphological properties of biomaterials affect the immune response. In the first part of this thesis, the uptake capacities of gold nanoparticles by the most important human primary leukocyte populations have been studied using a nanoparticle library encompassing both spherical and rod shaped gold nanoparticles. These studies were published (Bartneck et al. 2010a). The particles exhibited diameters between 15 and 50 nm as well as a variety of surface chemistries. Cetyl-trimethylammoniumbromide and citrate-stabilized nanoparticles were internalized rapidly within 15 minutes and in huge amounts by macrophages and monocytes. Interestingly, the uptake of CTAB-stabilized nanorods was more efficient than that of nanospheres. Blocking experiments and electron microscopic studies revealed macropinocytosis to be the underlying mechanism of this uptake. In addition to the studies on the intracellular uptake, a novel mechanism that results in the extracellular trapping of nanoparticles has been discovered and published (Bartneck et al. 2010b). It was shown that human neutrophils, monocytes and macrophages release extracellular traps which act as physical barriers for nanoparticles. The amount of particles which is trapped in the cell-gold networks strongly depends on the surface chemistry of the particles. Even PEG-coated nanoparticles are trapped to a significant extent by these extracellular structures. In the second part of this thesis, the impact of two-dimensional microtopographies on the inflammatory response of macrophages has been studied us…
abstract: The response of immune cells to any biomaterial determines its biocompatibility. This study aimed to identify the immunomodulatory properties of biomaterials from four different fields exhibiting various chemical, geometrical and morphological properties: nanoparticles, two-dimensional micropatterned structures, hydrogel with functional endgroups and three-dimensional nanofibers. It was shown in how far chemical, topographical and morphological properties of biomaterials affect the immune response. In the first part of this thesis, the uptake capacities of gold nanoparticles by the most important human primary leukocyte populations have been studied using a nanoparticle library encompassing both spherical and rod shaped gold nanoparticles. These studies were published (Bartneck et al. 2010a). The particles exhibited diameters between 15 and 50 nm as well as a variety of surface chemistries. Cetyl-trimethylammoniumbromide and citrate-stabilized nanoparticles were internalized rapidly within 15 minutes and in huge amounts by macrophages and monocytes. Interestingly, the uptake of CTAB-stabilized nanorods was more efficient than that of nanospheres. Blocking experiments and electron microscopic studies revealed macropinocytosis to be the underlying mechanism of this uptake. In addition to the studies on the intracellular uptake, a novel mechanism that results in the extracellular trapping of nanoparticles has been discovered and published (Bartneck et al. 2010b). It was shown that human neutrophils, monocytes and macrophages release extracellular traps which act as physical barriers for nanoparticles. The amount of particles which is trapped in the cell-gold networks strongly depends on the surface chemistry of the particles. Even PEG-coated nanoparticles are trapped to a significant extent by these extracellular structures. In the second part of this thesis, the impact of two-dimensional microtopographies on the inflammatory response of macrophages has been studied us…
author: Bartneck, Matthias
language: eng
keywords: info:eu-repo/classification/ddc/570, Entzündung, Biomaterial, Nanotechnologie, Differentielle Genexpression, Cytometrie, Biowissenschaften, Biologie, inflammation, nanotechnology, differential gene expression, cytometry
date published: 2010
date modified: 2026-07-30T19:43:35Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University