Vergleich der Differenzierungspotentiale humaner mesenchymaler Stammzellen aus dem Knochenmark und aus der Nabelschnur auf kollagenen Matrices
type: CreativeWork
description: The present dissertation compares the differentiation and migration potential as well as the functional behaviour of human bone marrow- and umbilical cord mesenchymal stem cells on three dimensional collagen scaffolds during osteogenic induction. We could draw conclusions from the in vitro characteristics of the stem cell-biomaterial-hybrids as to the in vivo integration of both stem cell types in human tissue. Both the perinatal and adult bone marrow stem cells exhibited all characteristics of mature osteoblasts, as it could be demonstrated by histology, immunhistochemistry, electron microscopy and molecular biology via RT-PCR. However, the BM-hMSC showed a stronger osteogenic transformation under two-dimensional culture conditions, whereas the UC-hMSC with contact to the collagenous matrix were superior to extracellular mineralisation and synthesis of extracellular matrix. In addition the UC-hMSC just like the BM-hMSC are able to boost fracture healing comparable with in vivo osteoblasts by the MMP-mediated migration and the associated colonization of the collagen scaffolds and by their great contractive potential. Insofar the mesenchymal stem cells from the umbilical cord will play a decisive role in the field of bone reconstruction in future. In order to realize their commitment not only in the field of mesodermal but also ectodermal tissues in long term, we tried to induce a differentiation of the UC-hMSC beyond the blastodermic layer. Therefore we cultivated the mesenchymal stem cells with air contact on dermal equivalents composed of collagen I/III with fibroblasts under epidermal differentiation stimuli. After isolation nearly 10% of the stem cells expressed the epithelial marker pan-cytoceratin, while this proportion increased to more than 30% after 21 days of three-dimensional culture conditions of skin, but after osteogenic stimulation there was no expression detectable. This underlinded the influence of the culture conditions. But except for the concomit…
abstract: The present dissertation compares the differentiation and migration potential as well as the functional behaviour of human bone marrow- and umbilical cord mesenchymal stem cells on three dimensional collagen scaffolds during osteogenic induction. We could draw conclusions from the in vitro characteristics of the stem cell-biomaterial-hybrids as to the in vivo integration of both stem cell types in human tissue. Both the perinatal and adult bone marrow stem cells exhibited all characteristics of mature osteoblasts, as it could be demonstrated by histology, immunhistochemistry, electron microscopy and molecular biology via RT-PCR. However, the BM-hMSC showed a stronger osteogenic transformation under two-dimensional culture conditions, whereas the UC-hMSC with contact to the collagenous matrix were superior to extracellular mineralisation and synthesis of extracellular matrix. In addition the UC-hMSC just like the BM-hMSC are able to boost fracture healing comparable with in vivo osteoblasts by the MMP-mediated migration and the associated colonization of the collagen scaffolds and by their great contractive potential. Insofar the mesenchymal stem cells from the umbilical cord will play a decisive role in the field of bone reconstruction in future. In order to realize their commitment not only in the field of mesodermal but also ectodermal tissues in long term, we tried to induce a differentiation of the UC-hMSC beyond the blastodermic layer. Therefore we cultivated the mesenchymal stem cells with air contact on dermal equivalents composed of collagen I/III with fibroblasts under epidermal differentiation stimuli. After isolation nearly 10% of the stem cells expressed the epithelial marker pan-cytoceratin, while this proportion increased to more than 30% after 21 days of three-dimensional culture conditions of skin, but after osteogenic stimulation there was no expression detectable. This underlinded the influence of the culture conditions. But except for the concomit…
author: Püllen, Andrea Susanne Kristina
language: ger
keywords: info:eu-repo/classification/ddc/610, Zelldifferenzierung, Adulte Stammzelle, Medizin, Differenzierungspotential, Migrationspotential, Transdifferenzierung, Nabelschnurstammzellen, Knochenmarkstammzellen, stem cells, differentiation potential, transdifferentiation
date published: 2011
date modified: 2026-07-30T19:43:35Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University