Die Subplacenta hystricognather Nager als In-vivo-Modell für die Trophoblastinvasion beim Menschen
type: CreativeWork
description: The aim of this pilot study is to test caviomorph placentation for its suitability as in-vivo-model for trophoblast invasion. The placentas of caviomorph rodents are characterized by the presence of a special organ,the subplacenta. The subplacenta is interposed between chorioallantoic placenta and uterine wall. Because of histological reasons, we assumed that the subplacenta is the source of throphoblast invasion and therefore an analogue to the cell columns in the human placenta. To test this assumption, we have applied the in-vivo proliferation marker BrdU (bromo-desoxy-uridine), as a thymidine analogue in guinea pig and degu pregnancy. 1-15 days later the placentas were studied immunohistochemically. We found out that following intraperitoneal injection into the mother we detected plenty BrdU-immunoreactivities in maternal cells in the uterine wall, but only little reactivitiy in trophoblast cells. The subplacenta was completely negative. By contrast strong Mib1 immunoreactivities suggested intense proliferation of cytothrophoblast in the subplacenta. The most logical explanation for this discrepancy was, that BrdU did not sufficiently pass the placental barrier. We therefore changed the procedure and injected Brdu fetally in the amnionic cavity. With this method, the subplacental cytotrophoblast was heavily stained, as expected and maternal cells were only rarely positive. As it is very difficult to aim the amnionic cavity, we finally came to the conclusion, that both ways the intraperitoneal and the intraamnial injection lead to better ways of positive immunreaction. Still the application is not easy and therefore it is not always possible to mark the throphoblast cells in the subplacenta. BrdU is stably incorporated into the nuclear DNA. Because of that, it persists in the cells after they have left the cell cycle. And it remains detectable when the cells invade maternal tissues. Accordingly, the days after application, we found marked trophoblast cells deep i…
abstract: The aim of this pilot study is to test caviomorph placentation for its suitability as in-vivo-model for trophoblast invasion. The placentas of caviomorph rodents are characterized by the presence of a special organ,the subplacenta. The subplacenta is interposed between chorioallantoic placenta and uterine wall. Because of histological reasons, we assumed that the subplacenta is the source of throphoblast invasion and therefore an analogue to the cell columns in the human placenta. To test this assumption, we have applied the in-vivo proliferation marker BrdU (bromo-desoxy-uridine), as a thymidine analogue in guinea pig and degu pregnancy. 1-15 days later the placentas were studied immunohistochemically. We found out that following intraperitoneal injection into the mother we detected plenty BrdU-immunoreactivities in maternal cells in the uterine wall, but only little reactivitiy in trophoblast cells. The subplacenta was completely negative. By contrast strong Mib1 immunoreactivities suggested intense proliferation of cytothrophoblast in the subplacenta. The most logical explanation for this discrepancy was, that BrdU did not sufficiently pass the placental barrier. We therefore changed the procedure and injected Brdu fetally in the amnionic cavity. With this method, the subplacental cytotrophoblast was heavily stained, as expected and maternal cells were only rarely positive. As it is very difficult to aim the amnionic cavity, we finally came to the conclusion, that both ways the intraperitoneal and the intraamnial injection lead to better ways of positive immunreaction. Still the application is not easy and therefore it is not always possible to mark the throphoblast cells in the subplacenta. BrdU is stably incorporated into the nuclear DNA. Because of that, it persists in the cells after they have left the cell cycle. And it remains detectable when the cells invade maternal tissues. Accordingly, the days after application, we found marked trophoblast cells deep i…
author: Zaki, Norma
language: ger
keywords: info:eu-repo/classification/ddc/610, trophoblast, BrdU, Trächtigkeit, Medizin, Trophoblastinvasion, Caviomorphe Nagetiere, in- vivo, Invasionsweg, Geschwindigkeit der Zellmigration, caviomorph rodents, in vivo, invasive pathway, speed of cellmigration
date published: 2007
date modified: 2026-07-30T19:43:28Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University