Untersuchung der parodontalen Manifestation von Morbus Crohn unter Berücksichtigung des NOD2(CARD15)-Genotyps und der Mikrobiologie
A correlation between crohn´s disease and periodontitis seems possible, because of the similarity concerning their clinical characterization. Previous studies which have dealt with this matter had different results.There are only a few studies that deal with patients who are only diseased with periodontis and crohns disease without other illnesses. Over the last years three genetic variations in the NOD2(Card15) genotype was associated with the crohn´s disease. The mutated gene produces a modified NOD2 protein which causes an unregulated inflammation. It has not been researched yet if there is an effection to the periodontal status. The aim of this study was to find out the effection of the NOD2(Card15) genotype mutation regarding the periodonal status and the microbiology of patients with crohns´s disease. 150 patients with crohn´s disease were recruited. Among other things probing depth and changes in the oral mucosa were detected. Samples from the periodontal pathogenic germs were taken with paper points and detected with the Dot-blotting hybridisation method. To find the NOD2(Card15) genetic mutation, blood samples were taken and were analysed by the TaqMan®-Assays. The connection between the clinical, microbiological and demographic parameters were figured out with a regression analysis. There were no statistically significant findings in the evaluation between the NOD2(Card15) gene mutation and the probing depth or attachment level. Among the patients the frequency of periodontits was high, but the severity was low or moderate.The important result was a high germ number of all periodontal pathagenous germs especially fom campylobactrer rectus. Even a high number of periodontal germs was found in healthy pockets. The quality and quantity of these germs were not statistically significant with the NOD2(Card15) genetic mutation. However the crohns disease seems to offer good living conditions for the germs. It is possible that the examined germs differ from the known species and that the crohns disease helps them to proliferate. The high number of germs could be important for the differential diagnosis between different bowel diseases and to estimate the genetic risk in families. Furthermore it is known that campylobacter rectus has got a s-layer protein on its surface which has a homology to heat shock proteins. These proteins are known for increasing a molecular mimikry. According to this the crohns disease could be an autoimmune disease caused by the heat schock proteins from campylobacter rectus.
info:eu-repo/classification/ddc/610, Morbus Crohn, Parodontitis, Medizin, NOD2, Crohn´s disease, periodontitis
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