Physikochemische und biologische Charakterisierung von texturiertem Silikon als Grundlage für die Entwicklung eines Retina-Patchs
type: CreativeWork
description: Retinal detachment (RD) and proliferative vitreoretinopathy (PVR) are serious eye diseases and lead to visual lost and blindness. They are caused by retinal breaks and foramina. The consequence is that the neurosensoric retina detaches from retinal pigment epithelium. Retina-Patchs in form of open-porous foils of polydimethylsiloxane (PDMS) could be a new possibility for closing the retinal breaks. It is intended that the Retina-Patch will be placed epiretinally at the retinal break and be fixed by proliferation of Müller cells (glial cells) located in the retina in the pores of the Retina-Patch. In this study, it was investigated whether textured silicone (TS) complies with the basic requirements to use it for the development of the ophthalomologic product Retina-Patch. Textured silicone is manufactured by the company Polytech-Silimed (Dieburg, D) in large-scale amount for the production of silicone-textured mamma implants. Samples of this material were provided by the company for this study. The textured silicone is characterized by non-porous and a porous side. The experimental part of this study worked on the physico-chemical and of TS and the growth of glial cells on this material in vitro. In detail, the micro structure of the surface texture, the hydrophilization of TS through surface modification, and the covalent protein immobilization were investigated. Furthermore, the cell growth behavior of glial cells were analyzed with the model of the cell line U373 (humane astrocytes). A further focus of this study was the investigation of the influence of anionic functionalization on the cell growth with the cell lines U373 and L929 (mice fibroblasts). The surface texture of TS is characterized by a big amount of pores. The pore size varies between 37 and 518 µm. The mean of the pore size is 84 µm. Small pores (60-90 µm) are most frequent. The inhomogeneity of the pore size distribution is caused by the manufacturing process. With contact angle measuring, the stabi…
abstract: Retinal detachment (RD) and proliferative vitreoretinopathy (PVR) are serious eye diseases and lead to visual lost and blindness. They are caused by retinal breaks and foramina. The consequence is that the neurosensoric retina detaches from retinal pigment epithelium. Retina-Patchs in form of open-porous foils of polydimethylsiloxane (PDMS) could be a new possibility for closing the retinal breaks. It is intended that the Retina-Patch will be placed epiretinally at the retinal break and be fixed by proliferation of Müller cells (glial cells) located in the retina in the pores of the Retina-Patch. In this study, it was investigated whether textured silicone (TS) complies with the basic requirements to use it for the development of the ophthalomologic product Retina-Patch. Textured silicone is manufactured by the company Polytech-Silimed (Dieburg, D) in large-scale amount for the production of silicone-textured mamma implants. Samples of this material were provided by the company for this study. The textured silicone is characterized by non-porous and a porous side. The experimental part of this study worked on the physico-chemical and of TS and the growth of glial cells on this material in vitro. In detail, the micro structure of the surface texture, the hydrophilization of TS through surface modification, and the covalent protein immobilization were investigated. Furthermore, the cell growth behavior of glial cells were analyzed with the model of the cell line U373 (humane astrocytes). A further focus of this study was the investigation of the influence of anionic functionalization on the cell growth with the cell lines U373 and L929 (mice fibroblasts). The surface texture of TS is characterized by a big amount of pores. The pore size varies between 37 and 518 µm. The mean of the pore size is 84 µm. Small pores (60-90 µm) are most frequent. The inhomogeneity of the pore size distribution is caused by the manufacturing process. With contact angle measuring, the stabi…
author: Schmidbauer, Michael
language: ger
keywords: info:eu-repo/classification/ddc/610, Tissue Engineering, Scaffold attached region, Oberflächenbehandlung, Implantatwerkstoff, Augenkrankheit, Netzhaut, Medizin, Retina-Patch, surface modification, retinal detachment
date published: 2007
date modified: 2026-07-30T19:43:28Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University