Untersuchungen zur Kryokonservierung humaner Adipozyten zur mehrzeitigen autologen Transplantation
type: CreativeWork
description: The autologous transplantation of cryopreserved human adipocytes is a usual technique in Plastic Surgery, up to now. There are no evidences, that these cells have normal vitality and a complete integrity of their membranes, at the moment of the transplantation. Aim of this work was, to make a quantitative statement about the cell-vitality and cell-integrity of mature human adipocytes, by using an enzyme-based test-system. Therefore, the existing MTT-, XTT- and GPDH-tests were modified for the use of human adipocytes. For the protection of human cells, during cryopreservation, cryoprotective agents are a common group of chemical substances, in science. In this work we used hydroxyethylstarch, glycerol and a mixture of glycerol, dextran and polyvinylpyrrolidone. The vitality of the cells was measured before the cryopreservation, at -20°C and -80°C, and after 2, 7 and 14 days as well as after 1, 6 and 12 month. As an intermediate-result we ascertained, that a cryopreservation of human adipocytes for 48 hours, without using a cryoprotective agent, leads to a vitality-loss of 94,9% (MTT-test, -20°C) and after one year of cryostorage to a vitality-loss of 97,4% (MTT-test, -20°C). Comparable results were detected in the XTT- and GPDH-test as well as by using -80°C as cryopreservation temperature. The use of the selected cryoprotectives was able to reduce the loss of cell-vitality and the cell-membrane damage, which was measured by using the GPDH-test. The best quantitative result was achieved by using the cryoprotective mixture. After one year of cryopreservation the cell-vitality was increased up to 30% in the MTT-test and up to 60% in the XTT-test; the GPDH-test could prove a reduction of the loss of cell-integrity up to 50%.1. The inset of cryopreserved human adipocytes, without using a cryoprotective agent, should be no subject of practical use in medicine.2. Through the addition of cryoprotective agents, it is possible to improve the vitality and integrity of human ad…
abstract: The autologous transplantation of cryopreserved human adipocytes is a usual technique in Plastic Surgery, up to now. There are no evidences, that these cells have normal vitality and a complete integrity of their membranes, at the moment of the transplantation. Aim of this work was, to make a quantitative statement about the cell-vitality and cell-integrity of mature human adipocytes, by using an enzyme-based test-system. Therefore, the existing MTT-, XTT- and GPDH-tests were modified for the use of human adipocytes. For the protection of human cells, during cryopreservation, cryoprotective agents are a common group of chemical substances, in science. In this work we used hydroxyethylstarch, glycerol and a mixture of glycerol, dextran and polyvinylpyrrolidone. The vitality of the cells was measured before the cryopreservation, at -20°C and -80°C, and after 2, 7 and 14 days as well as after 1, 6 and 12 month. As an intermediate-result we ascertained, that a cryopreservation of human adipocytes for 48 hours, without using a cryoprotective agent, leads to a vitality-loss of 94,9% (MTT-test, -20°C) and after one year of cryostorage to a vitality-loss of 97,4% (MTT-test, -20°C). Comparable results were detected in the XTT- and GPDH-test as well as by using -80°C as cryopreservation temperature. The use of the selected cryoprotectives was able to reduce the loss of cell-vitality and the cell-membrane damage, which was measured by using the GPDH-test. The best quantitative result was achieved by using the cryoprotective mixture. After one year of cryopreservation the cell-vitality was increased up to 30% in the MTT-test and up to 60% in the XTT-test; the GPDH-test could prove a reduction of the loss of cell-integrity up to 50%.1. The inset of cryopreserved human adipocytes, without using a cryoprotective agent, should be no subject of practical use in medicine.2. Through the addition of cryoprotective agents, it is possible to improve the vitality and integrity of human ad…
author: Stoffels, Ingo
language: ger
keywords: info:eu-repo/classification/ddc/610, Autogene Transplantation, Fettgewebe, Kryokonservierung, adipocyte viability, autologous fat transfer, cryopreservation, cryoprotective agents, enzymatic measurements of adipocyte viability, Adipozyten, Kryoprotektive, Medizin
date published: 2007
date modified: 2026-07-30T19:43:19Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University