{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:62109"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:62109","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Intramyokardiale Expression von pro- und anti-inflammatorischen Zytokinen und von iNOS bei Säuglingen mit angeborenem Herzfehler","abstract":"Our aim was to investigate whether intramyocardial mRNA-expression of cytokines and of iNOS is stimulated by heart failure in infants with left-to-right shunt or by hypoxia due to congenital cardiac defects. 22 infants (mean age 6,3 months) with heart failure due to a large intraventricular left-to-right shunt because of VSD (n=7) and AVSD (n=8) or without heart failure but with Tetralogy of Fallot (n=7) were prospectively investigated. Myocardial biopsy was taken from right atrium during corrective surgery, before institution of cardiopulmonary bypass. Expression of cytokine mRNA (Interleukin (IL) 1beta, IL6, IL 8, IL 10, TNF alpha) and iNOS mRNA in the myocardium was analysed by rtPCR and PCR. We demonstrated mRNA-expression of cytokines and of iNOS in the myocardium of infants with heart failure and without heart failure but with hypoxia due to congenital cardiac defects. As the semiquantitative analysis showed, mRNA-expression of cytokines and iNOS in the myocardium of infants with AVSD was significantly stronger to those with VSD and Tetralogy of Fallot. But not only mRNA-expression of pro-inflammatory cytokines and iNOS could be demonstrated in the myocardium of infants with congenital cardiac defects but also mRNA-expression of anti-inflammatory IL10. Our data demonstrate that heart failure due to large left-to-right shunt in infants is associated with the intramyocardial expression of pro- and anti-inflammatory cytokines and of iNOS. As these cytokines have strong negative inotropic effects, they could play an important role in the pathophysiology of heart failure in this age group.","abstract_html":"Our aim was to investigate whether intramyocardial mRNA-expression of cytokines and of iNOS is stimulated by heart failure in infants with left-to-right shunt or by hypoxia due to congenital cardiac defects. 22 infants (mean age 6,3 months) with heart failure due to a large intraventricular left-to-right shunt because of VSD (n=7) and AVSD (n=8) or without heart failure but with Tetralogy of Fallot (n=7) were prospectively investigated. Myocardial biopsy was taken from right atrium during corrective surgery, before institution of cardiopulmonary bypass. Expression of cytokine mRNA (Interleukin (IL) 1beta, IL6, IL 8, IL 10, TNF alpha) and iNOS mRNA in the myocardium was analysed by rtPCR and PCR. We demonstrated mRNA-expression of cytokines and of iNOS in the myocardium of infants with heart failure and without heart failure but with hypoxia due to congenital cardiac defects. As the semiquantitative analysis showed, mRNA-expression of cytokines and iNOS in the myocardium of infants with AVSD was significantly stronger to those with VSD and Tetralogy of Fallot. But not only mRNA-expression of pro-inflammatory cytokines and iNOS could be demonstrated in the myocardium of infants with congenital cardiac defects but also mRNA-expression of anti-inflammatory IL10. Our data demonstrate that heart failure due to large left-to-right shunt in infants is associated with the intramyocardial expression of pro- and anti-inflammatory cytokines and of iNOS. As these cytokines have strong negative inotropic effects, they could play an important role in the pathophysiology of heart failure in this age group.","abstract_has_math":false,"creators":["Aranda-Carrero, Monika"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Seghaye, Marie-Christine"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2005,"date_issued":"2005","date_published":"2005","updated_at":"2026-07-30T19:43:19Z","subjects":["info:eu-repo/classification/ddc/610","Medizin","Zytokine","Herzinsuffizienz","angeborene Herzfehler"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123702%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123702%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123702%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/62109","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Seghaye, Marie-Christine"]},{"key":"dc:creator","label":"Author","values":["Aranda-Carrero, Monika"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2005"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-11251"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin","Zytokine","Herzinsuffizienz","angeborene Herzfehler"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/62109","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123702%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Our aim was to investigate whether intramyocardial mRNA-expression of cytokines and of iNOS is stimulated by heart failure in infants with left-to-right shunt or by hypoxia due to congenital cardiac defects. 22 infants (mean age 6,3 months) with heart failure due to a large intraventricular left-to-right shunt because of VSD (n=7) and AVSD (n=8) or without heart failure but with Tetralogy of Fallot (n=7) were prospectively investigated. Myocardial biopsy was taken from right atrium during corrective surgery, before institution of cardiopulmonary bypass. Expression of cytokine mRNA (Interleukin (IL) 1beta, IL6, IL 8, IL 10, TNF alpha) and iNOS mRNA in the myocardium was analysed by rtPCR and PCR. We demonstrated mRNA-expression of cytokines and of iNOS in the myocardium of infants with heart failure and without heart failure but with hypoxia due to congenital cardiac defects. As the semiquantitative analysis showed, mRNA-expression of cytokines and iNOS in the myocardium of infants with AVSD was significantly stronger to those with VSD and Tetralogy of Fallot. But not only mRNA-expression of pro-inflammatory cytokines and iNOS could be demonstrated in the myocardium of infants with congenital cardiac defects but also mRNA-expression of anti-inflammatory IL10. Our data demonstrate that heart failure due to large left-to-right shunt in infants is associated with the intramyocardial expression of pro- and anti-inflammatory cytokines and of iNOS. As these cytokines have strong negative inotropic effects, they could play an important role in the pathophysiology of heart failure in this age group."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University VI, 56 S. : Ill., graph. Darst. (2005). = Aachen, Techn. Hochsch., Diss., 2005"]},{"key":"dc:title","label":"Title","values":["Intramyokardiale Expression von pro- und anti-inflammatorischen Zytokinen und von iNOS bei Säuglingen mit angeborenem Herzfehler"]}]}],"canonical_facts":{"dc:contributor":["Seghaye, Marie-Christine"],"dc:coverage":["DE"],"dc:creator":["Aranda-Carrero, Monika"],"dc:date":["2005"],"dc:description":["Our aim was to investigate whether intramyocardial mRNA-expression of cytokines and of iNOS is stimulated by heart failure in infants with left-to-right shunt or by hypoxia due to congenital cardiac defects. 22 infants (mean age 6,3 months) with heart failure due to a large intraventricular left-to-right shunt because of VSD (n=7) and AVSD (n=8) or without heart failure but with Tetralogy of Fallot (n=7) were prospectively investigated. Myocardial biopsy was taken from right atrium during corrective surgery, before institution of cardiopulmonary bypass. Expression of cytokine mRNA (Interleukin (IL) 1beta, IL6, IL 8, IL 10, TNF alpha) and iNOS mRNA in the myocardium was analysed by rtPCR and PCR. We demonstrated mRNA-expression of cytokines and of iNOS in the myocardium of infants with heart failure and without heart failure but with hypoxia due to congenital cardiac defects. As the semiquantitative analysis showed, mRNA-expression of cytokines and iNOS in the myocardium of infants with AVSD was significantly stronger to those with VSD and Tetralogy of Fallot. But not only mRNA-expression of pro-inflammatory cytokines and iNOS could be demonstrated in the myocardium of infants with congenital cardiac defects but also mRNA-expression of anti-inflammatory IL10. Our data demonstrate that heart failure due to large left-to-right shunt in infants is associated with the intramyocardial expression of pro- and anti-inflammatory cytokines and of iNOS. As these cytokines have strong negative inotropic effects, they could play an important role in the pathophysiology of heart failure in this age group."],"dc:identifier":["https://publications.rwth-aachen.de/record/62109","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123702%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-11251"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University VI, 56 S. : Ill., graph. Darst. (2005). = Aachen, Techn. Hochsch., Diss., 2005"],"dc:subject":["info:eu-repo/classification/ddc/610","Medizin","Zytokine","Herzinsuffizienz","angeborene Herzfehler"],"dc:title":["Intramyokardiale Expression von pro- und anti-inflammatorischen Zytokinen und von iNOS bei Säuglingen mit angeborenem Herzfehler"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:43:19Z"}