Modifizierung von Wolle durch Enzyme mit dem Ziel der Griffverbesserung und Filzfreiausrüstung
type: CreativeWork
description: This study investigates the effect of different enzymes on wool top. Wool top was incubated with proteases SP 655, SP 656, SP 490 as well as with lipase SP 398. The changes in wool morphology that occurred as a result of the enzyme treatment were shown by means of scanning electron microscopy and transmission electron microscopy. On the basis of the morphological results protease SP 655 was chosen for further experiments to improve the wool properties like handle, dyeing, shrink resistance and whiteness. Wool cortex and cuticle are not significantly changed after treatment with protease SP 655 over a period of 1 h. This was confirmed by gel electrophoresis. By contrast, the densitogram of merino wool treated with protease SP 655 over a period of 6 h, showed no more peaks in the area of the low sulfur proteins with a molecular weight of 45-50 kD, but an increase of peak number in the area of the low molecular weight proteins. The handle of merino wool was improved by enzyme treatment with a protease concentration of 0,2 % over a period of 1 h at 57 °C. Moreover 72 % of the testing persons found that the softness of crossbred wool increased after this enzyme treatment compared to the reference sample. The felting of merino wool top is not reduced by the enzyme treatment described above. The felting of crossbred wool, however, was significantly reduced after enzyme treatment in comparison to the reference fibres and untreated crossbred wool. The shrink resistance of merino wool is significantly improved only after treatment with a protease concentration of 0,6 %. A further decrease of felting was achieved by enzyme treatment in sodium borate/HCl-buffer. The felt density of the wool fibres treated in borate/HCl-buffer is significantly lower in comparison to the felt density of the wool fibres treated in borate/acetate-buffer. The felting results of merino wool after different treatment times showed that the felt density decreases with treatment time. Non-extracted wool …
abstract: This study investigates the effect of different enzymes on wool top. Wool top was incubated with proteases SP 655, SP 656, SP 490 as well as with lipase SP 398. The changes in wool morphology that occurred as a result of the enzyme treatment were shown by means of scanning electron microscopy and transmission electron microscopy. On the basis of the morphological results protease SP 655 was chosen for further experiments to improve the wool properties like handle, dyeing, shrink resistance and whiteness. Wool cortex and cuticle are not significantly changed after treatment with protease SP 655 over a period of 1 h. This was confirmed by gel electrophoresis. By contrast, the densitogram of merino wool treated with protease SP 655 over a period of 6 h, showed no more peaks in the area of the low sulfur proteins with a molecular weight of 45-50 kD, but an increase of peak number in the area of the low molecular weight proteins. The handle of merino wool was improved by enzyme treatment with a protease concentration of 0,2 % over a period of 1 h at 57 °C. Moreover 72 % of the testing persons found that the softness of crossbred wool increased after this enzyme treatment compared to the reference sample. The felting of merino wool top is not reduced by the enzyme treatment described above. The felting of crossbred wool, however, was significantly reduced after enzyme treatment in comparison to the reference fibres and untreated crossbred wool. The shrink resistance of merino wool is significantly improved only after treatment with a protease concentration of 0,6 %. A further decrease of felting was achieved by enzyme treatment in sodium borate/HCl-buffer. The felt density of the wool fibres treated in borate/HCl-buffer is significantly lower in comparison to the felt density of the wool fibres treated in borate/acetate-buffer. The felting results of merino wool after different treatment times showed that the felt density decreases with treatment time. Non-extracted wool …
author: Hollfelder, Barbara
language: ger
keywords: info:eu-repo/classification/ddc/540, Wolle, Modifizierung, Proteasen, Filzfreiausrüstung, Griffigkeit, Stoffeigenschaft, Chemie, Enzyme, Griffverbesserung
date published: 2003
date modified: 2026-07-30T19:43:19Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University