{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:61898"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:61898","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Der diagnostische Stellenwert der MIB-1- und PCNA-Immunhistometrie in der Differentialdiagnostik follikulärer Schilddrüsentumoren","abstract":"The diagnostic value of MIB-1 and PCNA immunohistometry in differential diagnosis of follicular tumors of the thyroid gland The cellular prolifarative activity MIB-1 and PCNA immunopositivity of normal tissue (n = 30), follicular adenoma (n = 21) and follicular carcinoma (n = 28) of the thyroid gland was analysed by means of immunohistometry. Immunohistochemical reactions were performed on 3-µm sections from routinely formalin fixed and paraffin embedded surgical specimens using the indirect peroxidase method. The rate of immunostained cells was determined using the CM - 2 TV image analysis system (Hund, Wetzlar, Federal Repuplic of Germany). Mean MIB-1and mean PCNA immunopositivity was higher in follicular carcinoma (MIB-1mean 2,52%, PCNAmean 1,49%) and in follicular adenoma (MIB-1mean 0,69%, PCNAmean 0,42%) than in normal thyroid tissue (MIB-1mean 0,14%, PCNAmean 0,05%).The distribution of single values differed significantly between groups. To test the suitability of MIB-1 and PCNA immunohistometry for differential diagnosis of follicular adenoma and follicular carcinoma, different four-field tables with varying thresholds were calculated. Using a threshold for MIB-1mean of 0,8%, follicular carcinoma could be detected with a sensitivity of 82,1% (23/28) and a specivity of 76,2% (16/21). Using a threshold for MIB-1mean of 1,3% a specivity of 90,5% is reached while the sensitivity decreases to 64,3%. With a PCNAmean threshold of 0,5% a follicular carcinoma could be detected with a sensitivity of 64,3% (18/28) and a specivity of 71,4% (15/21). Using a threshold for PCNAmean of 0,9% a specivity of over 90% is reached (90,5%; 18/21) while the sensitivity decreases to only 50% (14/28). A great overlap of low single values for MIB-1mean and PCNAmean was found in follicular adenoma and follicular carcinoma of the thyroid gland. Only one follicular adenoma showed a higher MIB-1mean immunopositivity than 2% and only one follicular adenoma had a PCNAmean immunopositivity of more than 1%. MIB-1 and PCNA immunohistometry cannot be recommended for differential diagnosis of follicular lesions in routine surgical pathology. In difficult differential diagnosis between follicular adenoma and carcinoma a MIB-1mean value > 2% and a PCNamean value > 1% could be a hint for malignancy and more tumor material should be embedded and / or serial sections should be performed in order not to miss rupture of the tumor capsule and / or infiltration of the blood vessels.","abstract_html":"The diagnostic value of MIB-1 and PCNA immunohistometry in differential diagnosis of follicular tumors of the thyroid gland The cellular prolifarative activity MIB-1 and PCNA immunopositivity of normal tissue (n = 30), follicular adenoma (n = 21) and follicular carcinoma (n = 28) of the thyroid gland was analysed by means of immunohistometry. Immunohistochemical reactions were performed on 3-µm sections from routinely formalin fixed and paraffin embedded surgical specimens using the indirect peroxidase method. The rate of immunostained cells was determined using the CM - 2 TV image analysis system (Hund, Wetzlar, Federal Repuplic of Germany). Mean MIB-1and mean PCNA immunopositivity was higher in follicular carcinoma (MIB-1mean 2,52%, PCNAmean 1,49%) and in follicular adenoma (MIB-1mean 0,69%, PCNAmean 0,42%) than in normal thyroid tissue (MIB-1mean 0,14%, PCNAmean 0,05%).The distribution of single values differed significantly between groups. To test the suitability of MIB-1 and PCNA immunohistometry for differential diagnosis of follicular adenoma and follicular carcinoma, different four-field tables with varying thresholds were calculated. Using a threshold for MIB-1mean of 0,8%, follicular carcinoma could be detected with a sensitivity of 82,1% (23/28) and a specivity of 76,2% (16/21). Using a threshold for MIB-1mean of 1,3% a specivity of 90,5% is reached while the sensitivity decreases to 64,3%. With a PCNAmean threshold of 0,5% a follicular carcinoma could be detected with a sensitivity of 64,3% (18/28) and a specivity of 71,4% (15/21). Using a threshold for PCNAmean of 0,9% a specivity of over 90% is reached (90,5%; 18/21) while the sensitivity decreases to only 50% (14/28). A great overlap of low single values for MIB-1mean and PCNAmean was found in follicular adenoma and follicular carcinoma of the thyroid gland. Only one follicular adenoma showed a higher MIB-1mean immunopositivity than 2% and only one follicular adenoma had a PCNAmean immunopositivity of more than 1%. MIB-1 and PCNA immunohistometry cannot be recommended for differential diagnosis of follicular lesions in routine surgical pathology. In difficult differential diagnosis between follicular adenoma and carcinoma a MIB-1mean value > 2% and a PCNamean value > 1% could be a hint for malignancy and more tumor material should be embedded and / or serial sections should be performed in order not to miss rupture of the tumor capsule and / or infiltration of the blood vessels.","abstract_has_math":false,"creators":["Eichler, Silke"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Biesterfeld, Stefan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004","date_published":"2004","updated_at":"2026-07-30T19:43:19Z","subjects":["info:eu-repo/classification/ddc/610","Medizin","MIB-1","PCNA","follikuläre Schilddrüsentumore"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123512%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123512%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123512%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/61898","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Biesterfeld, Stefan"]},{"key":"dc:creator","label":"Author","values":["Eichler, Silke"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2004"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-20042648"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin","MIB-1","PCNA","follikuläre Schilddrüsentumore"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/61898","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123512%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The diagnostic value of MIB-1 and PCNA immunohistometry in differential diagnosis of follicular tumors of the thyroid gland The cellular prolifarative activity MIB-1 and PCNA immunopositivity of normal tissue (n = 30), follicular adenoma (n = 21) and follicular carcinoma (n = 28) of the thyroid gland was analysed by means of immunohistometry. Immunohistochemical reactions were performed on 3-µm sections from routinely formalin fixed and paraffin embedded surgical specimens using the indirect peroxidase method. The rate of immunostained cells was determined using the CM - 2 TV image analysis system (Hund, Wetzlar, Federal Repuplic of Germany). Mean MIB-1and mean PCNA immunopositivity was higher in follicular carcinoma (MIB-1mean 2,52%, PCNAmean 1,49%) and in follicular adenoma (MIB-1mean 0,69%, PCNAmean 0,42%) than in normal thyroid tissue (MIB-1mean 0,14%, PCNAmean 0,05%).The distribution of single values differed significantly between groups. To test the suitability of MIB-1 and PCNA immunohistometry for differential diagnosis of follicular adenoma and follicular carcinoma, different four-field tables with varying thresholds were calculated. Using a threshold for MIB-1mean of 0,8%, follicular carcinoma could be detected with a sensitivity of 82,1% (23/28) and a specivity of 76,2% (16/21). Using a threshold for MIB-1mean of 1,3% a specivity of 90,5% is reached while the sensitivity decreases to 64,3%. With a PCNAmean threshold of 0,5% a follicular carcinoma could be detected with a sensitivity of 64,3% (18/28) and a specivity of 71,4% (15/21). Using a threshold for PCNAmean of 0,9% a specivity of over 90% is reached (90,5%; 18/21) while the sensitivity decreases to only 50% (14/28). A great overlap of low single values for MIB-1mean and PCNAmean was found in follicular adenoma and follicular carcinoma of the thyroid gland. Only one follicular adenoma showed a higher MIB-1mean immunopositivity than 2% and only one follicular adenoma had a PCNAmean immunopositivity of more than 1%. MIB-1 and PCNA immunohistometry cannot be recommended for differential diagnosis of follicular lesions in routine surgical pathology. In difficult differential diagnosis between follicular adenoma and carcinoma a MIB-1mean value > 2% and a PCNamean value > 1% could be a hint for malignancy and more tumor material should be embedded and / or serial sections should be performed in order not to miss rupture of the tumor capsule and / or infiltration of the blood vessels."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University IV, 90 S. : graph. Darst. (2004). = Aachen, Techn. Hochsch., Diss., 2004"]},{"key":"dc:title","label":"Title","values":["Der diagnostische Stellenwert der MIB-1- und PCNA-Immunhistometrie in der Differentialdiagnostik follikulärer Schilddrüsentumoren"]}]}],"canonical_facts":{"dc:contributor":["Biesterfeld, Stefan"],"dc:coverage":["DE"],"dc:creator":["Eichler, Silke"],"dc:date":["2004"],"dc:description":["The diagnostic value of MIB-1 and PCNA immunohistometry in differential diagnosis of follicular tumors of the thyroid gland The cellular prolifarative activity MIB-1 and PCNA immunopositivity of normal tissue (n = 30), follicular adenoma (n = 21) and follicular carcinoma (n = 28) of the thyroid gland was analysed by means of immunohistometry. Immunohistochemical reactions were performed on 3-µm sections from routinely formalin fixed and paraffin embedded surgical specimens using the indirect peroxidase method. The rate of immunostained cells was determined using the CM - 2 TV image analysis system (Hund, Wetzlar, Federal Repuplic of Germany). Mean MIB-1and mean PCNA immunopositivity was higher in follicular carcinoma (MIB-1mean 2,52%, PCNAmean 1,49%) and in follicular adenoma (MIB-1mean 0,69%, PCNAmean 0,42%) than in normal thyroid tissue (MIB-1mean 0,14%, PCNAmean 0,05%).The distribution of single values differed significantly between groups. To test the suitability of MIB-1 and PCNA immunohistometry for differential diagnosis of follicular adenoma and follicular carcinoma, different four-field tables with varying thresholds were calculated. Using a threshold for MIB-1mean of 0,8%, follicular carcinoma could be detected with a sensitivity of 82,1% (23/28) and a specivity of 76,2% (16/21). Using a threshold for MIB-1mean of 1,3% a specivity of 90,5% is reached while the sensitivity decreases to 64,3%. With a PCNAmean threshold of 0,5% a follicular carcinoma could be detected with a sensitivity of 64,3% (18/28) and a specivity of 71,4% (15/21). Using a threshold for PCNAmean of 0,9% a specivity of over 90% is reached (90,5%; 18/21) while the sensitivity decreases to only 50% (14/28). A great overlap of low single values for MIB-1mean and PCNAmean was found in follicular adenoma and follicular carcinoma of the thyroid gland. Only one follicular adenoma showed a higher MIB-1mean immunopositivity than 2% and only one follicular adenoma had a PCNAmean immunopositivity of more than 1%. MIB-1 and PCNA immunohistometry cannot be recommended for differential diagnosis of follicular lesions in routine surgical pathology. In difficult differential diagnosis between follicular adenoma and carcinoma a MIB-1mean value > 2% and a PCNamean value > 1% could be a hint for malignancy and more tumor material should be embedded and / or serial sections should be performed in order not to miss rupture of the tumor capsule and / or infiltration of the blood vessels."],"dc:identifier":["https://publications.rwth-aachen.de/record/61898","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123512%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-20042648"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University IV, 90 S. : graph. Darst. (2004). = Aachen, Techn. Hochsch., Diss., 2004"],"dc:subject":["info:eu-repo/classification/ddc/610","Medizin","MIB-1","PCNA","follikuläre Schilddrüsentumore"],"dc:title":["Der diagnostische Stellenwert der MIB-1- und PCNA-Immunhistometrie in der Differentialdiagnostik follikulärer Schilddrüsentumoren"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:43:19Z"}