Transport of metabolic active bacteria through saturated quartz sand columns with and without substrate addition
type: CreativeWork
description: Nowadays environmental protection not only refers to prevention of chemical pollution but also to biological contamination, which occurs due to manure or sludge on agricultural land. The released bacterial biomass is not negligible. The knowledge and the prediction of the behaviour of the released microorganisms in the upper soil as well as the deposition in lower soil compartments and the possible entry into the groundwater are of great interest, especially in case of pathogenic microorganisms. These transport pathways have been the subject of several investigations in the recent years. Experiments were carried out in different size scales: in columns, lysimeters, both filled with sand or soil, and outdoor tests. In all cases a so-called breakthrough curve (BTC) is calculated via analysis of the eluate. This BTC is characteristic for the chosen setup in means of conditions, e.g. column size, soil type, microorganism strain. At the moment a prediction of the transport behaviour is not possible, but there are competing theoretical models, from which calculations of microorganism distribution over a certain soil in the upper soil regions under the surface can be observed. But stringent boundary conditions (bacteria are treated as inactive particles) neglect the effects of bacterial properties on transport processes. Therefore predictions are only of limited value because they do not take into account effects of substrate, growth or stress. In this work the transport behaviour of metabolic active bacteria of Pseudomonas fluorescens in saturated quartz sand columns were investigated. The effect of the age of the used culture on the distribution of the bacteria between eluate and column was quantified. Furthermore the effect of substrate-addition under the same conditions was evaluated. For real-time measurements the used strain was genetically modified by chromosomal anchorage of the green fluorescent protein (gfp). Thus, in the eluate bacterial concentrations per time …
abstract: Nowadays environmental protection not only refers to prevention of chemical pollution but also to biological contamination, which occurs due to manure or sludge on agricultural land. The released bacterial biomass is not negligible. The knowledge and the prediction of the behaviour of the released microorganisms in the upper soil as well as the deposition in lower soil compartments and the possible entry into the groundwater are of great interest, especially in case of pathogenic microorganisms. These transport pathways have been the subject of several investigations in the recent years. Experiments were carried out in different size scales: in columns, lysimeters, both filled with sand or soil, and outdoor tests. In all cases a so-called breakthrough curve (BTC) is calculated via analysis of the eluate. This BTC is characteristic for the chosen setup in means of conditions, e.g. column size, soil type, microorganism strain. At the moment a prediction of the transport behaviour is not possible, but there are competing theoretical models, from which calculations of microorganism distribution over a certain soil in the upper soil regions under the surface can be observed. But stringent boundary conditions (bacteria are treated as inactive particles) neglect the effects of bacterial properties on transport processes. Therefore predictions are only of limited value because they do not take into account effects of substrate, growth or stress. In this work the transport behaviour of metabolic active bacteria of Pseudomonas fluorescens in saturated quartz sand columns were investigated. The effect of the age of the used culture on the distribution of the bacteria between eluate and column was quantified. Furthermore the effect of substrate-addition under the same conditions was evaluated. For real-time measurements the used strain was genetically modified by chromosomal anchorage of the green fluorescent protein (gfp). Thus, in the eluate bacterial concentrations per time …
author: Ralfs, Carla
language: eng
keywords: info:eu-repo/classification/ddc/540, Bodenbakterien, Boden, Chemie, Transport, Mikroorganismen, microorganisms
date published: 2007
date modified: 2026-07-30T19:43:10Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University