{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:61443"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:61443","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Einfluss von Haptoglobin auf Adhäsion/Invasion von CD11b-positiven Leukozyten an/in extrazelluläre Matrices","abstract":"Background: Haptoglobin has been discussed to play a role in the embryo-maternal interface during early pregnancy. Although a function of haptoglobin on macrophage adherence has been proposed (Sharpe-Timms 2002), the direct mechanism has not been investigated. Up to now limited information on the amount of haptoglobin in uterine secretions depending on the cyclic stage are available. Methods: The amount of haptoglobin from ovulation to menstruation in uterine secretions has been determined by quantitative Western blotting. Furthermore the influence of 0, 10, 50, 100 µg/ml haptoglobin1-1 or 2-2 on the adhesion of CD11b positive leukocytes to an extracelluar matrix was investigated. Results: At day 18/19 of the cycle lowest levels of haptoglobin in uterine secretions where detected (12.9 ng/5µg protein; ß-haptoglobin). This was followed by a significant increase at periimplantation time to highest levels measured (26.9 ng/5µg protein; ß-haptoglobin). Both, haptoglobin1-1- and 2-2 significantly reduced the binding of CD11b positive leukocytes to extracelluar matrix. Nevertheless, haptoglobin1-1 was more effective than 2-2. Conclusion: Haptoglobin is reducing the binding of leukocytes to extracellular matrices via CD11b receptor. In uterine secretion during secretion phase about 130 to 220 ng/µl haptoglobin are detectable. This amounts are highly effective to reduce leukocyte binding to ECMs.","abstract_html":"Background: Haptoglobin has been discussed to play a role in the embryo-maternal interface during early pregnancy. Although a function of haptoglobin on macrophage adherence has been proposed (Sharpe-Timms 2002), the direct mechanism has not been investigated. Up to now limited information on the amount of haptoglobin in uterine secretions depending on the cyclic stage are available. Methods: The amount of haptoglobin from ovulation to menstruation in uterine secretions has been determined by quantitative Western blotting. Furthermore the influence of 0, 10, 50, 100 µg/ml haptoglobin1-1 or 2-2 on the adhesion of CD11b positive leukocytes to an extracelluar matrix was investigated. Results: At day 18/19 of the cycle lowest levels of haptoglobin in uterine secretions where detected (12.9 ng/5µg protein; ß-haptoglobin). This was followed by a significant increase at periimplantation time to highest levels measured (26.9 ng/5µg protein; ß-haptoglobin). Both, haptoglobin1-1- and 2-2 significantly reduced the binding of CD11b positive leukocytes to extracelluar matrix. Nevertheless, haptoglobin1-1 was more effective than 2-2. Conclusion: Haptoglobin is reducing the binding of leukocytes to extracellular matrices via CD11b receptor. In uterine secretion during secretion phase about 130 to 220 ng/µl haptoglobin are detectable. This amounts are highly effective to reduce leukocyte binding to ECMs.","abstract_has_math":false,"creators":["Nauta, Ulrike Elisabeth Maria"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Herrler, Andreas"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2006,"date_issued":"2006","date_published":"2006","updated_at":"2026-07-30T19:43:10Z","subjects":["info:eu-repo/classification/ddc/610","Medizin","Leukozyt","Schwangerschaft","Immunmodulation","Haptoglobin","CD11b","uterine Sekretion","extracelluläre Matrices","uterine secretion","leucocytes","pregnancy"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123106%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123106%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123106%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/61443","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Herrler, Andreas"]},{"key":"dc:creator","label":"Author","values":["Nauta, Ulrike Elisabeth Maria"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2006"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-16151"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin","Leukozyt","Schwangerschaft","Immunmodulation","Haptoglobin","CD11b","uterine Sekretion","extracelluläre Matrices","uterine secretion","leucocytes","pregnancy"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/61443","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123106%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Background: Haptoglobin has been discussed to play a role in the embryo-maternal interface during early pregnancy. Although a function of haptoglobin on macrophage adherence has been proposed (Sharpe-Timms 2002), the direct mechanism has not been investigated. Up to now limited information on the amount of haptoglobin in uterine secretions depending on the cyclic stage are available. Methods: The amount of haptoglobin from ovulation to menstruation in uterine secretions has been determined by quantitative Western blotting. Furthermore the influence of 0, 10, 50, 100 µg/ml haptoglobin1-1 or 2-2 on the adhesion of CD11b positive leukocytes to an extracelluar matrix was investigated. Results: At day 18/19 of the cycle lowest levels of haptoglobin in uterine secretions where detected (12.9 ng/5µg protein; ß-haptoglobin). This was followed by a significant increase at periimplantation time to highest levels measured (26.9 ng/5µg protein; ß-haptoglobin). Both, haptoglobin1-1- and 2-2 significantly reduced the binding of CD11b positive leukocytes to extracelluar matrix. Nevertheless, haptoglobin1-1 was more effective than 2-2. Conclusion: Haptoglobin is reducing the binding of leukocytes to extracellular matrices via CD11b receptor. In uterine secretion during secretion phase about 130 to 220 ng/µl haptoglobin are detectable. This amounts are highly effective to reduce leukocyte binding to ECMs."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 58 S. : Ill., graph. Darst. (2006). = Aachen, Techn. Hochsch., Diss., 2006"]},{"key":"dc:title","label":"Title","values":["Einfluss von Haptoglobin auf Adhäsion/Invasion von CD11b-positiven Leukozyten an/in extrazelluläre Matrices"]}]}],"canonical_facts":{"dc:contributor":["Herrler, Andreas"],"dc:coverage":["DE"],"dc:creator":["Nauta, Ulrike Elisabeth Maria"],"dc:date":["2006"],"dc:description":["Background: Haptoglobin has been discussed to play a role in the embryo-maternal interface during early pregnancy. Although a function of haptoglobin on macrophage adherence has been proposed (Sharpe-Timms 2002), the direct mechanism has not been investigated. Up to now limited information on the amount of haptoglobin in uterine secretions depending on the cyclic stage are available. Methods: The amount of haptoglobin from ovulation to menstruation in uterine secretions has been determined by quantitative Western blotting. Furthermore the influence of 0, 10, 50, 100 µg/ml haptoglobin1-1 or 2-2 on the adhesion of CD11b positive leukocytes to an extracelluar matrix was investigated. Results: At day 18/19 of the cycle lowest levels of haptoglobin in uterine secretions where detected (12.9 ng/5µg protein; ß-haptoglobin). This was followed by a significant increase at periimplantation time to highest levels measured (26.9 ng/5µg protein; ß-haptoglobin). Both, haptoglobin1-1- and 2-2 significantly reduced the binding of CD11b positive leukocytes to extracelluar matrix. Nevertheless, haptoglobin1-1 was more effective than 2-2. Conclusion: Haptoglobin is reducing the binding of leukocytes to extracellular matrices via CD11b receptor. In uterine secretion during secretion phase about 130 to 220 ng/µl haptoglobin are detectable. This amounts are highly effective to reduce leukocyte binding to ECMs."],"dc:identifier":["https://publications.rwth-aachen.de/record/61443","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-123106%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-16151"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 58 S. : Ill., graph. Darst. (2006). = Aachen, Techn. Hochsch., Diss., 2006"],"dc:subject":["info:eu-repo/classification/ddc/610","Medizin","Leukozyt","Schwangerschaft","Immunmodulation","Haptoglobin","CD11b","uterine Sekretion","extracelluläre Matrices","uterine secretion","leucocytes","pregnancy"],"dc:title":["Einfluss von Haptoglobin auf Adhäsion/Invasion von CD11b-positiven Leukozyten an/in extrazelluläre Matrices"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:43:10Z"}