{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:60847"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:60847","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Expression, Reinigung und therapeutischer Einsatz eines löslichen TGF-[beta]-Typ-II-Rezeptors in der experimentell induzierten Leberfibrose","abstract":"The aim of this work was the production and testing of a soluble tgf-beta type II receptor as a pure protein in the therapy of the experimental induced liverfibrosis. Tgf-beta is as a mastercytocine responsible for the transdifferentiation of hepatic stellate cells to myofiborblasts, the key-event of liverfibrosis. The protein may intercept tgf-beta before binding the hepatic stellate cells. For amplification of the protein COS-7 cells were infected with the adenoviral construct Ad5-CMV-sTbetaRII. The supernatant of the cells was purified with the HiTrap Protein G HP 1 ml column. By using 4x10exp11 pfu virus an amount of 1 g pure protein was harvested. The biological effect was proved by a PAI-1/luciferase-assay. In vitro testing happened by transdifferentiation assay with activated hepatic stellate cells. In vivo testing was performed by bile-duct-ligation induced liverfibrosis in male sprague dawley rats (mean bodyweight 250 g). Over a time-period of 14 days 0.1 mg/kg of the pure protein were administered by one intravenous and four intraperitoneal injections. For collagen I was a significant suppression here in northern-blot shown in animals treated with the protein in contrast to the control-group. 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In vivo testing was performed by bile-duct-ligation induced liverfibrosis in male sprague dawley rats (mean bodyweight 250 g). Over a time-period of 14 days 0.1 mg/kg of the pure protein were administered by one intravenous and four intraperitoneal injections. For collagen I was a significant suppression here in northern-blot shown in animals treated with the protein in contrast to the control-group. 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