{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:60092"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:60092","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Analyse der Annexin-1-Expression und Identifikation neuer Glukokortikoid-stimulierbarer Gene durch Differential-Display-RT-PCR-Methodik in humanen Makrophagenkulturen","abstract":"The molecular mode of action of the antiphlogistic properties of glucocorticoids has been subject of intense investigation for many years. Certain processes lead to changes in the transcription of different genes, inter alia of those genes which encode for proteins and which are to transmit a part of the antiphlogistic effects of the glucocorticoids, as is the case with Annexin-1. In the first part of the present study we examined the influence of the glucocorticoid prednylidene on the Annexin-1 expression in human monocytes and their cultures (macrophagocytes). The expression of metallothionin-2 served as reference for the effects of the glucocorticoids on the mRNA-level whereas we used the RM 3/1- antigen which is specific of the macrophage as reference on the protein level. In the second part of the study we searched for new genes which are induced or stimulated by glucocorticoids in monocytes/macrophages, using the differential-display-RT-PCR-method. The results show that neither on the gene level nor on the protein level the production of Annexin 1 in human monocytes and monocytic cultures is regulated by glucocorticoids. These results call into question its assumed property as second messenger molecule for the antiphlogistic effects of glucocorticoids, at least in the case of macrophages. These findings rather suggest that Annexin-1 constitutes a constitutive cytosol protein in these cells. The findings also show that a considerable number of genes in macrophages is triggered or highly regulated by glucocorticoids. With the help of the differential-display-RT-PCR-method we could not only discover already known glucocorticoid-regulated genes as metallothionin-2 or the RM 3/1-CD163 scavenger receptor but also new genes such as the catalase or other hitherto unknown genes.","abstract_html":"The molecular mode of action of the antiphlogistic properties of glucocorticoids has been subject of intense investigation for many years. Certain processes lead to changes in the transcription of different genes, inter alia of those genes which encode for proteins and which are to transmit a part of the antiphlogistic effects of the glucocorticoids, as is the case with Annexin-1. In the first part of the present study we examined the influence of the glucocorticoid prednylidene on the Annexin-1 expression in human monocytes and their cultures (macrophagocytes). The expression of metallothionin-2 served as reference for the effects of the glucocorticoids on the mRNA-level whereas we used the RM 3/1- antigen which is specific of the macrophage as reference on the protein level. In the second part of the study we searched for new genes which are induced or stimulated by glucocorticoids in monocytes/macrophages, using the differential-display-RT-PCR-method. The results show that neither on the gene level nor on the protein level the production of Annexin 1 in human monocytes and monocytic cultures is regulated by glucocorticoids. These results call into question its assumed property as second messenger molecule for the antiphlogistic effects of glucocorticoids, at least in the case of macrophages. These findings rather suggest that Annexin-1 constitutes a constitutive cytosol protein in these cells. The findings also show that a considerable number of genes in macrophages is triggered or highly regulated by glucocorticoids. With the help of the differential-display-RT-PCR-method we could not only discover already known glucocorticoid-regulated genes as metallothionin-2 or the RM 3/1-CD163 scavenger receptor but also new genes such as the catalase or other hitherto unknown genes.","abstract_has_math":false,"creators":["Zsolnai, Andreas"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Zwadlo-Klarwasser, Gabriele-Claudia"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001","date_published":"2001","updated_at":"2026-07-30T19:42:48Z","subjects":["info:eu-repo/classification/ddc/610","Medizin"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121820%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121820%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121820%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/60092","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Zwadlo-Klarwasser, Gabriele-Claudia"]},{"key":"dc:creator","label":"Author","values":["Zsolnai, Andreas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2001"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-1415"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/60092","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121820%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The molecular mode of action of the antiphlogistic properties of glucocorticoids has been subject of intense investigation for many years. Certain processes lead to changes in the transcription of different genes, inter alia of those genes which encode for proteins and which are to transmit a part of the antiphlogistic effects of the glucocorticoids, as is the case with Annexin-1. In the first part of the present study we examined the influence of the glucocorticoid prednylidene on the Annexin-1 expression in human monocytes and their cultures (macrophagocytes). The expression of metallothionin-2 served as reference for the effects of the glucocorticoids on the mRNA-level whereas we used the RM 3/1- antigen which is specific of the macrophage as reference on the protein level. In the second part of the study we searched for new genes which are induced or stimulated by glucocorticoids in monocytes/macrophages, using the differential-display-RT-PCR-method. The results show that neither on the gene level nor on the protein level the production of Annexin 1 in human monocytes and monocytic cultures is regulated by glucocorticoids. These results call into question its assumed property as second messenger molecule for the antiphlogistic effects of glucocorticoids, at least in the case of macrophages. These findings rather suggest that Annexin-1 constitutes a constitutive cytosol protein in these cells. The findings also show that a considerable number of genes in macrophages is triggered or highly regulated by glucocorticoids. With the help of the differential-display-RT-PCR-method we could not only discover already known glucocorticoid-regulated genes as metallothionin-2 or the RM 3/1-CD163 scavenger receptor but also new genes such as the catalase or other hitherto unknown genes."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 95 S. : Ill. (2001). = Aachen, Techn. Hochsch., Diss., 2001"]},{"key":"dc:title","label":"Title","values":["Analyse der Annexin-1-Expression und Identifikation neuer Glukokortikoid-stimulierbarer Gene durch Differential-Display-RT-PCR-Methodik in humanen Makrophagenkulturen"]}]}],"canonical_facts":{"dc:contributor":["Zwadlo-Klarwasser, Gabriele-Claudia"],"dc:coverage":["DE"],"dc:creator":["Zsolnai, Andreas"],"dc:date":["2001"],"dc:description":["The molecular mode of action of the antiphlogistic properties of glucocorticoids has been subject of intense investigation for many years. Certain processes lead to changes in the transcription of different genes, inter alia of those genes which encode for proteins and which are to transmit a part of the antiphlogistic effects of the glucocorticoids, as is the case with Annexin-1. In the first part of the present study we examined the influence of the glucocorticoid prednylidene on the Annexin-1 expression in human monocytes and their cultures (macrophagocytes). The expression of metallothionin-2 served as reference for the effects of the glucocorticoids on the mRNA-level whereas we used the RM 3/1- antigen which is specific of the macrophage as reference on the protein level. In the second part of the study we searched for new genes which are induced or stimulated by glucocorticoids in monocytes/macrophages, using the differential-display-RT-PCR-method. The results show that neither on the gene level nor on the protein level the production of Annexin 1 in human monocytes and monocytic cultures is regulated by glucocorticoids. These results call into question its assumed property as second messenger molecule for the antiphlogistic effects of glucocorticoids, at least in the case of macrophages. These findings rather suggest that Annexin-1 constitutes a constitutive cytosol protein in these cells. The findings also show that a considerable number of genes in macrophages is triggered or highly regulated by glucocorticoids. With the help of the differential-display-RT-PCR-method we could not only discover already known glucocorticoid-regulated genes as metallothionin-2 or the RM 3/1-CD163 scavenger receptor but also new genes such as the catalase or other hitherto unknown genes."],"dc:identifier":["https://publications.rwth-aachen.de/record/60092","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121820%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-1415"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 95 S. : Ill. (2001). = Aachen, Techn. 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