{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:59899"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:59899","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Calciumkanaldichte in humanem ischämischen und reperfundierten, rechtsatrialen Myokardgewebe","abstract":"Intracellular calcium overload is one of the major pathophysiological mechanisms contributing to myocardial ischemic injury. Two important calcium handling proteins responsible for the maintenance of an intracellular calcium homeostasis are the sarcolemmal voltage-gated L-type calcium channel (DHPR) and the sarcoplasmic reticulum calcium release channel (CRC). This study evaluated the density of both channels in human myocardium subjected to ischemia and reperfusion during cardia surgery. Biopsies of human right atria were collected during cardiac surgery: 1) before aortic cross-clamping (serving as controls, 2) during ischemia and 3) after reperfusion. DHPR and CRC densities were studied using radioligand binding. A 25% reduction in CRC density was found during ischemia and reperfusion. The calcium sensitivity of ryanodine binding to CRC was unchanged. DHPR density was unaltered during ischemia and reperfusion. A downregulation of CRC may lead to a defective release of Ca2+ from the sarcoplasmic reticulum which may lead to excitation-contraction uncoupling ultimately contributing to postischemic contractile dysfunction.","abstract_html":"Intracellular calcium overload is one of the major pathophysiological mechanisms contributing to myocardial ischemic injury. Two important calcium handling proteins responsible for the maintenance of an intracellular calcium homeostasis are the sarcolemmal voltage-gated L-type calcium channel (DHPR) and the sarcoplasmic reticulum calcium release channel (CRC). This study evaluated the density of both channels in human myocardium subjected to ischemia and reperfusion during cardia surgery. Biopsies of human right atria were collected during cardiac surgery: 1) before aortic cross-clamping (serving as controls, 2) during ischemia and 3) after reperfusion. DHPR and CRC densities were studied using radioligand binding. A 25% reduction in CRC density was found during ischemia and reperfusion. The calcium sensitivity of ryanodine binding to CRC was unchanged. DHPR density was unaltered during ischemia and reperfusion. A downregulation of CRC may lead to a defective release of Ca2+ from the sarcoplasmic reticulum which may lead to excitation-contraction uncoupling ultimately contributing to postischemic contractile dysfunction.","abstract_has_math":false,"creators":["Greiser, Birte Maura"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Hanrath, Peter"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001","date_published":"2001","updated_at":"2026-07-30T19:42:48Z","subjects":["info:eu-repo/classification/ddc/610","Medizin"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121640%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121640%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121640%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/59899","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hanrath, Peter"]},{"key":"dc:creator","label":"Author","values":["Greiser, Birte Maura"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2001"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-1972"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/610","Medizin"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/59899","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121640%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Intracellular calcium overload is one of the major pathophysiological mechanisms contributing to myocardial ischemic injury. Two important calcium handling proteins responsible for the maintenance of an intracellular calcium homeostasis are the sarcolemmal voltage-gated L-type calcium channel (DHPR) and the sarcoplasmic reticulum calcium release channel (CRC). This study evaluated the density of both channels in human myocardium subjected to ischemia and reperfusion during cardia surgery. Biopsies of human right atria were collected during cardiac surgery: 1) before aortic cross-clamping (serving as controls, 2) during ischemia and 3) after reperfusion. DHPR and CRC densities were studied using radioligand binding. A 25% reduction in CRC density was found during ischemia and reperfusion. The calcium sensitivity of ryanodine binding to CRC was unchanged. DHPR density was unaltered during ischemia and reperfusion. A downregulation of CRC may lead to a defective release of Ca2+ from the sarcoplasmic reticulum which may lead to excitation-contraction uncoupling ultimately contributing to postischemic contractile dysfunction."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 71 S. : graph. Darst. (2001). = Aachen, Techn. Hochsch., Diss., 2001"]},{"key":"dc:title","label":"Title","values":["Calciumkanaldichte in humanem ischämischen und reperfundierten, rechtsatrialen Myokardgewebe"]}]}],"canonical_facts":{"dc:contributor":["Hanrath, Peter"],"dc:coverage":["DE"],"dc:creator":["Greiser, Birte Maura"],"dc:date":["2001"],"dc:description":["Intracellular calcium overload is one of the major pathophysiological mechanisms contributing to myocardial ischemic injury. Two important calcium handling proteins responsible for the maintenance of an intracellular calcium homeostasis are the sarcolemmal voltage-gated L-type calcium channel (DHPR) and the sarcoplasmic reticulum calcium release channel (CRC). This study evaluated the density of both channels in human myocardium subjected to ischemia and reperfusion during cardia surgery. Biopsies of human right atria were collected during cardiac surgery: 1) before aortic cross-clamping (serving as controls, 2) during ischemia and 3) after reperfusion. DHPR and CRC densities were studied using radioligand binding. A 25% reduction in CRC density was found during ischemia and reperfusion. The calcium sensitivity of ryanodine binding to CRC was unchanged. DHPR density was unaltered during ischemia and reperfusion. 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