{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:59603"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:59603","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Klonierung und Charakterisierung des PCC1-Gens ('Pathogen and circadian controlled 1')","abstract":"Despite of a significant progress in understanding the defense signal transduction in plants, especially in Arabidopsis thaliana, there are still many open questions and new components to identify. A tool, to identify so far not characterized components of the signal-transduction-cascade is the usage of \"microarrays\". This was done by Scheideler et al. (2002) who compared the gene expression between mock- and avirulent P. syringae (avr Rpt2)-treated Arabidopsis plants. I used these data to identify so far unknown A. thaliana-genes with a potential role in pathogen defense. We re-tested the differential gene expression of 30 potentially differentially expressed genes and identified an EST, which was induced reliably after pathogen-treatment. In this thesis, I present the detailed characterization of EST 163B24T7. As a result of my studies, I could demonstrate, that the gene corresponding to the EST was not only induced after pathogen treatment. Furthermore, the mRNA-levels were fluctuating even in untreated plants in a daily manner with a maximum of mRNA-levels at the end of the day. In addition, further analysis demonstrated that the rhythmic expression kept fluctuating under constant light conditions, indicating a circadian clock controlled expression profile. Accordingly, we named the gene PCC1 (Pathogen and Clock Controlled1). In a 35S::CCA1-line, in which many circadian processes are disturbed in constant light conditions, the expression profile of PCC1 was also disordered, corroborating our idea of circadian clock control. I was able to show, that pathogen-induction of PCC1 is salicylic acid (SA) and NPR1 (\"nonexpressor of PR1\")-dependent. Only the npr1-mutant and the transgenic NahG-line failed to induce the PCC1-gene after treatment with P. syringae pv. tomato (avrRpt2), whereas in the rps2-101C, ndr1-1, eds1-2, pad4-2 and ein2 plants an induction of PCC1-RNA-levels was detectable. Even the circadian-rhythmic expression of the PCC1 was not detectable in npr1 and NahG-plants, which suggests, that SA and npr1 fulfill a crucial role in the circadian control of PCC1-expression as well as in pathogen-defense. These data suggest, that PCC1 is like PR1 and PR5 positioned downstream of NPR1 and salicylic acid. These findings were supported by an in-silico analysis of the promotor-elements of PR1, PR5 and PCC1. Crucial elements of the PR1-promotor like the as1-element and the W-box were also found in the promoter of PCC1. 35S::PCC1 and PCC1-RNAi-silencing constructs and plants were generated, to see, if PCC1-overexpression or PCC1silencing leads to an observable phenotype.35S::PCC1 plants seemed to show resistance against the Hyaloperonospora parasitica isolate Noco, whereas the corresponding wild-type was susceptible. These results have to be verified with a new generation of 35S::PCC1-plants, since it was realized, that there had been seed contamination in the pool of plants, which were originally transformed with the 35S-PCC1-construct.. Although effective PCC1-RNAi-silencing was confirmed in 3 independent PCC1-silencing lines, there was no differential phenotype according the resistance against the H. parasitica isolates Noco and Wela. The molecular function of PCC1 is still unclear, but PCC1 may act as a PR-protein like PR1, PR5 etc. downstream of NPR1 and salicylic acid.","abstract_html":"Despite of a significant progress in understanding the defense signal transduction in plants, especially in Arabidopsis thaliana, there are still many open questions and new components to identify. A tool, to identify so far not characterized components of the signal-transduction-cascade is the usage of "microarrays". This was done by Scheideler et al. (2002) who compared the gene expression between mock- and avirulent P. syringae (avr Rpt2)-treated Arabidopsis plants. I used these data to identify so far unknown A. thaliana-genes with a potential role in pathogen defense. We re-tested the differential gene expression of 30 potentially differentially expressed genes and identified an EST, which was induced reliably after pathogen-treatment. In this thesis, I present the detailed characterization of EST 163B24T7. As a result of my studies, I could demonstrate, that the gene corresponding to the EST was not only induced after pathogen treatment. Furthermore, the mRNA-levels were fluctuating even in untreated plants in a daily manner with a maximum of mRNA-levels at the end of the day. In addition, further analysis demonstrated that the rhythmic expression kept fluctuating under constant light conditions, indicating a circadian clock controlled expression profile. Accordingly, we named the gene PCC1 (Pathogen and Clock Controlled1). In a 35S::CCA1-line, in which many circadian processes are disturbed in constant light conditions, the expression profile of PCC1 was also disordered, corroborating our idea of circadian clock control. I was able to show, that pathogen-induction of PCC1 is salicylic acid (SA) and NPR1 ("nonexpressor of PR1")-dependent. Only the npr1-mutant and the transgenic NahG-line failed to induce the PCC1-gene after treatment with P. syringae pv. tomato (avrRpt2), whereas in the rps2-101C, ndr1-1, eds1-2, pad4-2 and ein2 plants an induction of PCC1-RNA-levels was detectable. Even the circadian-rhythmic expression of the PCC1 was not detectable in npr1 and NahG-plants, which suggests, that SA and npr1 fulfill a crucial role in the circadian control of PCC1-expression as well as in pathogen-defense. These data suggest, that PCC1 is like PR1 and PR5 positioned downstream of NPR1 and salicylic acid. These findings were supported by an in-silico analysis of the promotor-elements of PR1, PR5 and PCC1. Crucial elements of the PR1-promotor like the as1-element and the W-box were also found in the promoter of PCC1. 35S::PCC1 and PCC1-RNAi-silencing constructs and plants were generated, to see, if PCC1-overexpression or PCC1silencing leads to an observable phenotype.35S::PCC1 plants seemed to show resistance against the Hyaloperonospora parasitica isolate Noco, whereas the corresponding wild-type was susceptible. These results have to be verified with a new generation of 35S::PCC1-plants, since it was realized, that there had been seed contamination in the pool of plants, which were originally transformed with the 35S-PCC1-construct.. Although effective PCC1-RNAi-silencing was confirmed in 3 independent PCC1-silencing lines, there was no differential phenotype according the resistance against the H. parasitica isolates Noco and Wela. The molecular function of PCC1 is still unclear, but PCC1 may act as a PR-protein like PR1, PR5 etc. downstream of NPR1 and salicylic acid.","abstract_has_math":false,"creators":["Sauerbrunn, Nicolas"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Slusarenko, Alan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004","date_published":"2004","updated_at":"2026-07-30T19:42:39Z","subjects":["info:eu-repo/classification/ddc/570","Ackerschmalwand","Abwehrreaktion","Resistenzgen","Genanalyse","Biowissenschaften, Biologie"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121377%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121377%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121377%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/59603","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Slusarenko, Alan"]},{"key":"dc:creator","label":"Author","values":["Sauerbrunn, Nicolas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["2004"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-9150"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/570","Ackerschmalwand","Abwehrreaktion","Resistenzgen","Genanalyse","Biowissenschaften, Biologie"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/59603","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121377%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Despite of a significant progress in understanding the defense signal transduction in plants, especially in Arabidopsis thaliana, there are still many open questions and new components to identify. A tool, to identify so far not characterized components of the signal-transduction-cascade is the usage of \"microarrays\". This was done by Scheideler et al. (2002) who compared the gene expression between mock- and avirulent P. syringae (avr Rpt2)-treated Arabidopsis plants. I used these data to identify so far unknown A. thaliana-genes with a potential role in pathogen defense. We re-tested the differential gene expression of 30 potentially differentially expressed genes and identified an EST, which was induced reliably after pathogen-treatment. In this thesis, I present the detailed characterization of EST 163B24T7. As a result of my studies, I could demonstrate, that the gene corresponding to the EST was not only induced after pathogen treatment. Furthermore, the mRNA-levels were fluctuating even in untreated plants in a daily manner with a maximum of mRNA-levels at the end of the day. In addition, further analysis demonstrated that the rhythmic expression kept fluctuating under constant light conditions, indicating a circadian clock controlled expression profile. Accordingly, we named the gene PCC1 (Pathogen and Clock Controlled1). In a 35S::CCA1-line, in which many circadian processes are disturbed in constant light conditions, the expression profile of PCC1 was also disordered, corroborating our idea of circadian clock control. I was able to show, that pathogen-induction of PCC1 is salicylic acid (SA) and NPR1 (\"nonexpressor of PR1\")-dependent. Only the npr1-mutant and the transgenic NahG-line failed to induce the PCC1-gene after treatment with P. syringae pv. tomato (avrRpt2), whereas in the rps2-101C, ndr1-1, eds1-2, pad4-2 and ein2 plants an induction of PCC1-RNA-levels was detectable. Even the circadian-rhythmic expression of the PCC1 was not detectable in npr1 and NahG-plants, which suggests, that SA and npr1 fulfill a crucial role in the circadian control of PCC1-expression as well as in pathogen-defense. These data suggest, that PCC1 is like PR1 and PR5 positioned downstream of NPR1 and salicylic acid. These findings were supported by an in-silico analysis of the promotor-elements of PR1, PR5 and PCC1. Crucial elements of the PR1-promotor like the as1-element and the W-box were also found in the promoter of PCC1. 35S::PCC1 and PCC1-RNAi-silencing constructs and plants were generated, to see, if PCC1-overexpression or PCC1silencing leads to an observable phenotype.35S::PCC1 plants seemed to show resistance against the Hyaloperonospora parasitica isolate Noco, whereas the corresponding wild-type was susceptible. These results have to be verified with a new generation of 35S::PCC1-plants, since it was realized, that there had been seed contamination in the pool of plants, which were originally transformed with the 35S-PCC1-construct.. Although effective PCC1-RNAi-silencing was confirmed in 3 independent PCC1-silencing lines, there was no differential phenotype according the resistance against the H. parasitica isolates Noco and Wela. The molecular function of PCC1 is still unclear, but PCC1 may act as a PR-protein like PR1, PR5 etc. downstream of NPR1 and salicylic acid."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University 149 S. : Ill. graph. Darst. (2004). = Aachen, Techn. Hochsch., Diss., 2004"]},{"key":"dc:title","label":"Title","values":["Klonierung und Charakterisierung des PCC1-Gens ('Pathogen and circadian controlled 1')"]}]}],"canonical_facts":{"dc:contributor":["Slusarenko, Alan"],"dc:coverage":["DE"],"dc:creator":["Sauerbrunn, Nicolas"],"dc:date":["2004"],"dc:description":["Despite of a significant progress in understanding the defense signal transduction in plants, especially in Arabidopsis thaliana, there are still many open questions and new components to identify. A tool, to identify so far not characterized components of the signal-transduction-cascade is the usage of \"microarrays\". This was done by Scheideler et al. (2002) who compared the gene expression between mock- and avirulent P. syringae (avr Rpt2)-treated Arabidopsis plants. I used these data to identify so far unknown A. thaliana-genes with a potential role in pathogen defense. We re-tested the differential gene expression of 30 potentially differentially expressed genes and identified an EST, which was induced reliably after pathogen-treatment. In this thesis, I present the detailed characterization of EST 163B24T7. As a result of my studies, I could demonstrate, that the gene corresponding to the EST was not only induced after pathogen treatment. Furthermore, the mRNA-levels were fluctuating even in untreated plants in a daily manner with a maximum of mRNA-levels at the end of the day. In addition, further analysis demonstrated that the rhythmic expression kept fluctuating under constant light conditions, indicating a circadian clock controlled expression profile. Accordingly, we named the gene PCC1 (Pathogen and Clock Controlled1). In a 35S::CCA1-line, in which many circadian processes are disturbed in constant light conditions, the expression profile of PCC1 was also disordered, corroborating our idea of circadian clock control. I was able to show, that pathogen-induction of PCC1 is salicylic acid (SA) and NPR1 (\"nonexpressor of PR1\")-dependent. Only the npr1-mutant and the transgenic NahG-line failed to induce the PCC1-gene after treatment with P. syringae pv. tomato (avrRpt2), whereas in the rps2-101C, ndr1-1, eds1-2, pad4-2 and ein2 plants an induction of PCC1-RNA-levels was detectable. Even the circadian-rhythmic expression of the PCC1 was not detectable in npr1 and NahG-plants, which suggests, that SA and npr1 fulfill a crucial role in the circadian control of PCC1-expression as well as in pathogen-defense. These data suggest, that PCC1 is like PR1 and PR5 positioned downstream of NPR1 and salicylic acid. These findings were supported by an in-silico analysis of the promotor-elements of PR1, PR5 and PCC1. Crucial elements of the PR1-promotor like the as1-element and the W-box were also found in the promoter of PCC1. 35S::PCC1 and PCC1-RNAi-silencing constructs and plants were generated, to see, if PCC1-overexpression or PCC1silencing leads to an observable phenotype.35S::PCC1 plants seemed to show resistance against the Hyaloperonospora parasitica isolate Noco, whereas the corresponding wild-type was susceptible. These results have to be verified with a new generation of 35S::PCC1-plants, since it was realized, that there had been seed contamination in the pool of plants, which were originally transformed with the 35S-PCC1-construct.. Although effective PCC1-RNAi-silencing was confirmed in 3 independent PCC1-silencing lines, there was no differential phenotype according the resistance against the H. parasitica isolates Noco and Wela. The molecular function of PCC1 is still unclear, but PCC1 may act as a PR-protein like PR1, PR5 etc. downstream of NPR1 and salicylic acid."],"dc:identifier":["https://publications.rwth-aachen.de/record/59603","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-121377%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-9150"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University 149 S. : Ill. graph. Darst. (2004). = Aachen, Techn. Hochsch., Diss., 2004"],"dc:subject":["info:eu-repo/classification/ddc/570","Ackerschmalwand","Abwehrreaktion","Resistenzgen","Genanalyse","Biowissenschaften, Biologie"],"dc:title":["Klonierung und Charakterisierung des PCC1-Gens ('Pathogen and circadian controlled 1')"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:42:39Z"}