Struktur und Funktion des GR1-Gens aus Arabidopsis thaliana (L.) Heynh
type: CreativeWork
description: Arabidopsis thaliana shows race-specific interactions with the obligate biotrophic oomycete Hyaloperonospora parasitica. Thus, accession Wassilewskija (Ws-0) is resistant to the isolates WELA and NOCO. The gr1 mutant was isolated from a T-DNA population of Ws-0 plants showing susceptibility towards the WELA isolate, however, retaining resistance towards the NOCO isolate. Since it seemed that the T-DNA insertion into the GR1 gene was the cause for the loss of resistance against the WELA isolate, it was the aim of this thesis project to characterize the GR1 gene and the gr1 mutant and to study the function of the gene and protein. Towards characterization of the GR1 gene, sites of transcription initiation and termination, typical elements of the GR1 promoter, and the translation initiation site should be identified. Using 5'RACE it was shown that the first of two ATGs at the 5'-end serves as the translation initiation site. Moreover, the likely transcription initiation site was localized 118bp upstream of the first ATG. Another 35bp upstream of the transcription initiation site there is an AT-rich region which could serve as the TATA-box. A potential CAAT-box, as a second regulatory element, was found 90bp upstream. Using 3'RACE, it was shown that there is no strictly defined end of transcription but that the transcripts end in AT-rich regions. Towards characterization of the gr1 mutant the precise position of the T-DNA insertion should be localized. Accordingly, the left-border of the T-DNA was found to be integrated 1843bp downstream of the ATG and additional 50bp non-GR1 sequence was found and showed to be present in frame. The right-border was localized in the third exon 1921bp downstream of the ATG. Since the left- and right-border have integrated at different sites in the gr1 mutant a deletion of 78bp in the third exon became apparent. To test, whether the mutated gene is responsible for the susceptibility-phenotype of the gr1 mutant to Hyaloperonospora parasiti…
abstract: Arabidopsis thaliana shows race-specific interactions with the obligate biotrophic oomycete Hyaloperonospora parasitica. Thus, accession Wassilewskija (Ws-0) is resistant to the isolates WELA and NOCO. The gr1 mutant was isolated from a T-DNA population of Ws-0 plants showing susceptibility towards the WELA isolate, however, retaining resistance towards the NOCO isolate. Since it seemed that the T-DNA insertion into the GR1 gene was the cause for the loss of resistance against the WELA isolate, it was the aim of this thesis project to characterize the GR1 gene and the gr1 mutant and to study the function of the gene and protein. Towards characterization of the GR1 gene, sites of transcription initiation and termination, typical elements of the GR1 promoter, and the translation initiation site should be identified. Using 5'RACE it was shown that the first of two ATGs at the 5'-end serves as the translation initiation site. Moreover, the likely transcription initiation site was localized 118bp upstream of the first ATG. Another 35bp upstream of the transcription initiation site there is an AT-rich region which could serve as the TATA-box. A potential CAAT-box, as a second regulatory element, was found 90bp upstream. Using 3'RACE, it was shown that there is no strictly defined end of transcription but that the transcripts end in AT-rich regions. Towards characterization of the gr1 mutant the precise position of the T-DNA insertion should be localized. Accordingly, the left-border of the T-DNA was found to be integrated 1843bp downstream of the ATG and additional 50bp non-GR1 sequence was found and showed to be present in frame. The right-border was localized in the third exon 1921bp downstream of the ATG. Since the left- and right-border have integrated at different sites in the gr1 mutant a deletion of 78bp in the third exon became apparent. To test, whether the mutated gene is responsible for the susceptibility-phenotype of the gr1 mutant to Hyaloperonospora parasiti…
author: Schmitz, Gudrun
language: ger
keywords: info:eu-repo/classification/ddc/570, Ackerschmalwand, Resistenzgen, Pathogene Pilze, Biowissenschaften, Biologie, Arabidopsis thaliana, Rassenspezifische Resistenz, 14-3-3 Proteine
date published: 2005
date modified: 2026-07-30T19:42:39Z
provider: RWTH Aachen University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Publikationsserver der RWTH Aachen University